Complexity of poly(A+) and poly(A-) polysomal RNA in mouse liver and cultured mouse fibroblasts (original) (raw)

Abstract

RNA excess hybridization experiments were used to measure the complexity of nuclear RNA, poly(A+) mRNA, poly(A-) mRNA, and EDTA-released polysomal RNA sedimenting at less than 80 S in mouse liver and in cultured mouse cells. With both cell types, poly(A-) RNA was found to contain 30-40% of the sequence diversity of total mRNA. In the case of liver this represents 5,700 poly(A-) molecules and 8,600 poly(A+) molecules for a total of approximately 14,300 different mRNAs. Comparison of the complexity of mRNA with that of nuclear RNA revealed that in liver and in cultured cells, mRNA has only 10-20% of the sequence diversity present in nuclear RNA. This latter observation is consistent with existing data on mammalian cells from this and other laboratories.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adesnik M., Salditt M., Thomas W., Darnell J. E. Evidence that all messenger RNA molecules (except histone messenger RNA) contain Poly (A) sequences and that the Poly(A) has a nuclear function. J Mol Biol. 1972 Oct 28;71(1):21–30. doi: 10.1016/0022-2836(72)90397-x. [DOI] [PubMed] [Google Scholar]
  2. Bantle J. A., Hahn W. E. Complexity and characterization of polyadenylated RNA in the mouse brain. Cell. 1976 May;8(1):139–150. doi: 10.1016/0092-8674(76)90195-1. [DOI] [PubMed] [Google Scholar]
  3. Beatriz L. W., McCarthy B. J. Messenger RNA complexity in Drosophila melanogaster. Biochemistry. 1975 Jun 3;14(11):2440–2446. doi: 10.1021/bi00682a026. [DOI] [PubMed] [Google Scholar]
  4. Bishop J. O., Morton J. G., Rosbash M., Richardson M. Three abundance classes in HeLa cell messenger RNA. Nature. 1974 Jul 19;250(463):199–204. doi: 10.1038/250199a0. [DOI] [PubMed] [Google Scholar]
  5. Brown I. R., Church R. B. RNA transcription from nonrepetitive DNA in the mouse. Biochem Biophys Res Commun. 1971 Mar 5;42(5):850–856. doi: 10.1016/0006-291x(71)90508-0. [DOI] [PubMed] [Google Scholar]
  6. Fromson D., Verma D. P. Translation of nonpolyadenylylated messenger RNA of sea urchin embryos. Proc Natl Acad Sci U S A. 1976 Jan;73(1):148–151. doi: 10.1073/pnas.73.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Galau G. A., Britten R. J., Davidson E. H. A measurement of the sequence complexity of polysomal messenger RNA in sea urchin embryos. Cell. 1974 May;2(1):9–20. doi: 10.1016/0092-8674(74)90003-8. [DOI] [PubMed] [Google Scholar]
  8. Garrett C. T., Wilkinson D. S., Pitot H. C. Preparation of ribonuclease-free DNase I and -amylase. Anal Biochem. 1973 Apr;52(2):342–348. doi: 10.1016/0003-2697(73)90035-3. [DOI] [PubMed] [Google Scholar]
  9. Getz M. J., Birnie G. D., Young B. D., MacPhail E., Paul J. A kinetic estimation of base sequence complexity of nuclear poly(A)-containing RNA in mouse Friend cells. Cell. 1975 Feb;4(2):121–129. doi: 10.1016/0092-8674(75)90118-x. [DOI] [PubMed] [Google Scholar]
  10. Getz M. J., Elder P. K., Benz E. W., Jr, Stephens R. E., Moses H. L. Effect of cell proliferation on levels and diversity of poly(A)-containing mRNA. Cell. 1976 Feb;7(2):255–65. doi: 10.1016/0092-8674(76)90025-8. [DOI] [PubMed] [Google Scholar]
  11. Grady L. J., Campbell W. P. Non-repetitive DNA transcription in mouse cells grown in tissue culture. Nat New Biol. 1973 Jun 13;243(128):195–198. doi: 10.1038/newbio243195a0. [DOI] [PubMed] [Google Scholar]
  12. Grady L. J., Campbell W. P. Non-repetitive DNA transcripts in nuclei and polysomes of polyomatransformed and non-transformed mouse cells. Nature. 1975 Mar 27;254(5498):356–358. doi: 10.1038/254356a0. [DOI] [PubMed] [Google Scholar]
  13. Grady L. J., Campbell W. P. Transcription of the repetitive DNA sequences in polyoma-transformed and nontransformed mouse cells in culture. Cancer Res. 1975 Jun;35(6):1559–1562. [PubMed] [Google Scholar]
  14. Grady L. J., North A. B., Campbell W. P. Polyoma genome transcription in transformed mouse cells growing in culture and as tumors in syngeneic mice. Int J Cancer. 1977 Feb 15;19(2):236–239. doi: 10.1002/ijc.2910190213. [DOI] [PubMed] [Google Scholar]
  15. Greenberg J. R. Isolation of L-cell messenger RNA which lacks poly(adenylate). Biochemistry. 1976 Aug 10;15(16):3516–3522. doi: 10.1021/bi00661a019. [DOI] [PubMed] [Google Scholar]
  16. Grouse L., Chilton M. D., McCarthy B. J. Hybridization of ribonucleic acid with unique sequences of mouse deoxyribonucleic acid. Biochemistry. 1972 Feb 29;11(5):798–805. doi: 10.1021/bi00755a019. [DOI] [PubMed] [Google Scholar]
  17. Hahn W. E., Laird C. D. Transcription of nonrepeated DNA in mouse brain. Science. 1971 Jul 9;173(3992):158–161. doi: 10.1126/science.173.3992.158. [DOI] [PubMed] [Google Scholar]
  18. Hastie N. D., Bishop J. O. The expression of three abundance classes of messenger RNA in mouse tissues. Cell. 1976 Dec;9(4 Pt 2):761–774. doi: 10.1016/0092-8674(76)90139-2. [DOI] [PubMed] [Google Scholar]
  19. Hough B. R., Smith M. J., Britten R. J., Davidson E. H. Sequence complexity of heterogeneous nuclear RNA in sea urchin embryos. Cell. 1975 Jul;5(3):291–299. doi: 10.1016/0092-8674(75)90104-x. [DOI] [PubMed] [Google Scholar]
  20. Lewin B. Units of transcription and translation: the relationship between heterogeneous nuclear RNA and messenger RNA. Cell. 1975 Jan;4(1):11–20. doi: 10.1016/0092-8674(75)90128-2. [DOI] [PubMed] [Google Scholar]
  21. MCCARTHY B. J., HOYER B. H. IDENTITY OF DNA AND DIVERSITY OF MESSENGER RNA MOLECULES IN NORMAL MOUSE TISSUES. Proc Natl Acad Sci U S A. 1964 Oct;52:915–922. doi: 10.1073/pnas.52.4.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Milcarek C., Price R., Penman S. The metabolism of a poly(A) minus mRNA fraction in HeLa cells. Cell. 1974 Sep;3(1):1–10. doi: 10.1016/0092-8674(74)90030-0. [DOI] [PubMed] [Google Scholar]
  23. Nemer M., Graham M., Dubroff L. M. Co-existence of non-histone messenger RNA species lacking and containing polyadenylic acid in sea urchin embryos. J Mol Biol. 1974 Nov 5;89(3):435–454. doi: 10.1016/0022-2836(74)90474-4. [DOI] [PubMed] [Google Scholar]
  24. Perry R. P., La Torre J., Kelley D. E., Greenberg J. R. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim Biophys Acta. 1972 Mar 14;262(2):220–226. doi: 10.1016/0005-2787(72)90236-5. [DOI] [PubMed] [Google Scholar]
  25. Purdom I., Williamson R., Birnstiel M. Kinetic analysis of the base sequence heterogeneity of RNA molecules by RNA-DNA hybridization. Results Probl Cell Differ. 1972;3:25–36. doi: 10.1007/978-3-540-37149-6_3. [DOI] [PubMed] [Google Scholar]
  26. Ryffel G. U., McCarthy B. J. Complexity of cytoplasmic RNA in different mouse tissues measured by hybridization of polyadenylated RNA to complementary DNA. Biochemistry. 1975 Apr 8;14(7):1379–1385. doi: 10.1021/bi00678a006. [DOI] [PubMed] [Google Scholar]
  27. Sippel A. E., Hynes N., Groner B., Schütz G. Frequency distribution of messenger sequences within polysomal mRNA and nuclear RNA from rat liver. Eur J Biochem. 1977 Jul 1;77(1):141–151. doi: 10.1111/j.1432-1033.1977.tb11652.x. [DOI] [PubMed] [Google Scholar]
  28. Williams J. G., Penman S. The messenger RNA sequences in growing and resting mouse fibroblasts. Cell. 1975 Oct;6(2):197–206. doi: 10.1016/0092-8674(75)90010-0. [DOI] [PubMed] [Google Scholar]
  29. Young B. D., Birnie G. D. Complexity and specificity of polysomal poly(A+) RNA in mouse tissues. Biochemistry. 1976 Jun 29;15(13):2823–2829. doi: 10.1021/bi00658a019. [DOI] [PubMed] [Google Scholar]