7-Methylguanine specific tRNA-methyltransferase from Escherichia coli (original) (raw)

Abstract

A 7-methylguanine (m7G) specific tRNA methyltransferase from E. coli MRE 600 was purified about 1000 fold by affinity chromatography on Sepharose bound with normal E. coli tRNA. The purified enzyme catalyzes exclusively the formation of m7G in submethylated bulk tRNA of E. coli K12 met- rel-. The purified enzyme transfers the methyl group from S-adenosyl-methionine to initiator tRNA of B. subtilis and 0.8 moles m7G residues are formed per mole tRNA. It is suggested that the enzyme specifically recognizes the extra arm unpaired guanylate residue.

3109

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem J. 1965 Sep;96(3):595–606. doi: 10.1042/bj0960595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnold H. H., Kersten H. Inhibition of the tetrahydrofolate-dependent biosynthesis of ribothymidine in tRNAs of B. subtilis and M. lysodeikticus by trimethoprim. FEBS Lett. 1975 May 1;53(2):258–261. doi: 10.1016/0014-5793(75)80032-9. [DOI] [PubMed] [Google Scholar]
  3. Arnold H., Kersten H. The occurrence of ribothymidine, 1-methyladenosine, methylated guanosines and the corresponding methyltransferases in E. coli and Bacillus subtilis. FEBS Lett. 1973 Oct 1;36(1):34–38. doi: 10.1016/0014-5793(73)80331-x. [DOI] [PubMed] [Google Scholar]
  4. Baguley B. C., Wehrli W., Staehelin M. In vitro methylation of yeast serine transfer ribonucleic acid. Biochemistry. 1970 Mar 31;9(7):1645–1649. doi: 10.1021/bi00809a026. [DOI] [PubMed] [Google Scholar]
  5. Dube S. K., Marcker K. A. The nucleotide sequence of N-formyl-methionyl-transfer RNA. Partial digestion with pancreatic and T-1 ribonuclease and derivation of the total primary structure. Eur J Biochem. 1969 Mar;8(2):256–262. doi: 10.1111/j.1432-1033.1969.tb00522.x. [DOI] [PubMed] [Google Scholar]
  6. FLEISSNER E., BOREK E. A new enzyme of RNA synthesis: RNA methylase. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1199–1203. doi: 10.1073/pnas.48.7.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HURWITZ J., GOLD M., ANDERS M. THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. 3. PURIFICATION OF SOLUBLE RIBONUCLEIC ACID-METHYLATING ENZYMES. J Biol Chem. 1964 Oct;239:3462–3473. [PubMed] [Google Scholar]
  8. Kerr S. J., Borek E. The tRNA methyltransferases. Adv Enzymol Relat Areas Mol Biol. 1972;36:1–27. doi: 10.1002/9780470122815.ch1. [DOI] [PubMed] [Google Scholar]
  9. Kersten H., Sandig L., Arnold H. H. Tetrahydrofolate-dependent 5-methyluracil-tRNA transferase activity in B. subtilis. FEBS Lett. 1975 Jul 15;55(1):57–60. doi: 10.1016/0014-5793(75)80956-2. [DOI] [PubMed] [Google Scholar]
  10. Kraus J., Staehelin M. N2-guanine specific transfer RNA methyltransferase I from rat liver and leukemic rat spleen. Nucleic Acids Res. 1974 Nov;1(11):1455–1478. doi: 10.1093/nar/1.11.1455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuchino Y., Nishimura S. Nucleotide sequence specificities of guanylate residue-specific tRNA methylases from rat liver. Biochem Biophys Res Commun. 1970 Jul 27;40(2):306–313. doi: 10.1016/0006-291x(70)91010-7. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Marinus M. G., Morris N. R., Söll D., Kwong T. C. Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases. J Bacteriol. 1975 Apr;122(1):257–265. doi: 10.1128/jb.122.1.257-265.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  15. Raettig R., Schmidt W., Mahal G., Kersten H., Arnold H. H. Purification and characterization of tRNAMet-f, tRNAPhe and tRNATyr2 from Baccillus subtilis. Biochim Biophys Acta. 1976 Jun 18;435(2):109–118. doi: 10.1016/0005-2787(76)90241-0. [DOI] [PubMed] [Google Scholar]
  16. Remy P., Birmelé C., Ebel J. P. Purification of yeast phenylalanyl-tRNA synthetase by affinity chromatography, on a tRNA(Phe)-sepharose column. FEBS Lett. 1972 Oct 15;27(1):134–138. doi: 10.1016/0014-5793(72)80426-5. [DOI] [PubMed] [Google Scholar]
  17. Romeo J. M., Delk A. S., Rabinowitz J. C. The occurrence of a transmethylation reaction not involving S-adenosylmethionine in the formation of ribothymidine in Bacillus subtilis transfer-RNA. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1256–1261. doi: 10.1016/s0006-291x(74)80419-5. [DOI] [PubMed] [Google Scholar]
  18. Schäfer K. P., Söll D. New aspects in tRNA biosynthesis. Biochimie. 1974;56(6-7):795–804. doi: 10.1016/s0300-9084(74)80500-6. [DOI] [PubMed] [Google Scholar]
  19. Shershneva L. P., Venkstern T. V., Bayev A. A. A study of tRNA methylase action. FEBS Lett. 1973 Jan 15;29(2):132–134. doi: 10.1016/0014-5793(73)80543-5. [DOI] [PubMed] [Google Scholar]
  20. Taya Y., Nishimura S. Biosynthesis of 5-methylaminomethyl-2-thiouridylate. I. Isolation of a new tRNA-methylase specific for 5-methylaminomethyl-2-thiouridylate. Biochem Biophys Res Commun. 1973 Apr 16;51(4):1062–1068. doi: 10.1016/0006-291x(73)90035-1. [DOI] [PubMed] [Google Scholar]
  21. Yamada Y., Ishikura H. Nucleotide sequence of initiator tRNA from Bacillus subtilis. FEBS Lett. 1975 Jun 15;54(2):155–158. doi: 10.1016/0014-5793(75)80064-0. [DOI] [PubMed] [Google Scholar]
  22. Yang W. K., Novelli G. D. Isoaccepting +RNA's in mouse plasma cell tumors that synthesize different myeloma protein. Biochem Biophys Res Commun. 1968 May 23;31(4):534–539. doi: 10.1016/0006-291x(68)90510-x. [DOI] [PubMed] [Google Scholar]