Molecular cloning of a human gene that is a member of the nerve growth factor family. (original) (raw)

• Journal List
• Proc Natl Acad Sci U S A
• v.87(20); 1990 Oct
• PMC54892

Proc Natl Acad Sci U S A. 1990 Oct; 87(20): 8060–8064.

Howard Hughes Medical Institute, University of California-San Francisco 94143.

Abstract

Cell death within the developing vertebrate nervous system is regulated in part by interactions between neurons and their innervation targets that are mediated by neurotrophic factors. These factors also appear to have a role in the maintenance of the adult nervous system. Two neurotrophic factors, nerve growth factor and brain-derived neurotrophic factor, share substantial amino acid sequence identity. We have used a screen that combines polymerase chain reaction amplification of genomic DNA and low-stringency hybridization with degenerate oligonucleotides to isolate human BDNF and a human gene, neurotrophin-3, that is closely related to both nerve growth factor and brain-derived neurotrophic factor. mRNA products of the brain-derived neurotrophic factor and neurotrophin-3 genes were detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. Neurotrophin-3 is also expected to function in embryonic neural development.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

• Barde YA. Trophic factors and neuronal survival. Neuron. 1989 Jun;2(6):1525–1534. [PubMed] [Google Scholar]
• Hamburger V, Yip JW. Reduction of experimentally induced neuronal death in spinal ganglia of the chick embryo by nerve growth factor. J Neurosci. 1984 Mar;4(3):767–774. [PMC free article] [PubMed] [Google Scholar]
• Hofer MM, Barde YA. Brain-derived neurotrophic factor prevents neuronal death in vivo. Nature. 1988 Jan 21;331(6153):261–262. [PubMed] [Google Scholar]
• Lindsay RM, Lockett C, Sternberg J, Winter J. Neuropeptide expression in cultures of adult sensory neurons: modulation of substance P and calcitonin gene-related peptide levels by nerve growth factor. Neuroscience. 1989;33(1):53–65. [PubMed] [Google Scholar]
• Purves D, Snider WD, Voyvodic JT. Trophic regulation of nerve cell morphology and innervation in the autonomic nervous system. Nature. 1988 Nov 10;336(6195):123–128. [PubMed] [Google Scholar]
• Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [PubMed] [Google Scholar]
• Nathans J, Hogness DS. Isolation and nucleotide sequence of the gene encoding human rhodopsin. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4851–4855. [PMC free article] [PubMed] [Google Scholar]
• Lewis SA, Gilmartin ME, Hall JL, Cowan NJ. Three expressed sequences within the human beta-tubulin multigene family each define a distinct isotype. J Mol Biol. 1985 Mar 5;182(1):11–20. [PubMed] [Google Scholar]
• Ullrich A, Gray A, Berman C, Dull TJ. Human beta-nerve growth factor gene sequence highly homologous to that of mouse. Nature. 1983 Jun 30;303(5920):821–825. [PubMed] [Google Scholar]
• von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690. [PMC free article] [PubMed] [Google Scholar]
• Padgett RA, Grabowski PJ, Konarska MM, Seiler S, Sharp PA. Splicing of messenger RNA precursors. Annu Rev Biochem. 1986;55:1119–1150. [PubMed] [Google Scholar]
• Leibrock J, Lottspeich F, Hohn A, Hofer M, Hengerer B, Masiakowski P, Thoenen H, Barde YA. Molecular cloning and expression of brain-derived neurotrophic factor. Nature. 1989 Sep 14;341(6238):149–152. [PubMed] [Google Scholar]
• Ebendal T, Larhammar D, Persson H. Structure and expression of the chicken beta nerve growth factor gene. EMBO J. 1986 Jul;5(7):1483–1487. [PMC free article] [PubMed] [Google Scholar]
• Edwards RH, Selby MJ, Garcia PD, Rutter WJ. Processing of the native nerve growth factor precursor to form biologically active nerve growth factor. J Biol Chem. 1988 May 15;263(14):6810–6815. [PubMed] [Google Scholar]
• Selby MJ, Edwards R, Sharp F, Rutter WJ. Mouse nerve growth factor gene: structure and expression. Mol Cell Biol. 1987 Sep;7(9):3057–3064. [PMC free article] [PubMed] [Google Scholar]
• Gray AM, Mason AJ. Requirement for activin A and transforming growth factor--beta 1 pro-regions in homodimer assembly. Science. 1990 Mar 16;247(4948):1328–1330. [PubMed] [Google Scholar]
• Hohn A, Leibrock J, Bailey K, Barde YA. Identification and characterization of a novel member of the nerve growth factor/brain-derived neurotrophic factor family. Nature. 1990 Mar 22;344(6264):339–341. [PubMed] [Google Scholar]
• Maisonpierre PC, Belluscio L, Squinto S, Ip NY, Furth ME, Lindsay RM, Yancopoulos GD. Neurotrophin-3: a neurotrophic factor related to NGF and BDNF. Science. 1990 Mar 23;247(4949 Pt 1):1446–1451. [PubMed] [Google Scholar]
• Rodriguez-Tébar A, Dechant G, Barde YA. Binding of brain-derived neurotrophic factor to the nerve growth factor receptor. Neuron. 1990 Apr;4(4):487–492. [PubMed] [Google Scholar]
• Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M. Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860–873. [PMC free article] [PubMed] [Google Scholar]
• Yan Q, Snider WD, Pinzone JJ, Johnson EM., Jr Retrograde transport of nerve growth factor (NGF) in motoneurons of developing rats: assessment of potential neurotrophic effects. Neuron. 1988 Jun;1(4):335–343. [PubMed] [Google Scholar]
• Lindsay RM, Thoenen H, Barde YA. Placode and neural crest-derived sensory neurons are responsive at early developmental stages to brain-derived neurotrophic factor. Dev Biol. 1985 Dec;112(2):319–328. [PubMed] [Google Scholar]
• Davies AM, Thoenen H, Barde YA. The response of chick sensory neurons to brain-derived neurotrophic factor. J Neurosci. 1986 Jul;6(7):1897–1904. [PMC free article] [PubMed] [Google Scholar]
• Davies AM, Thoenen H, Barde YA. Different factors from the central nervous system and periphery regulate the survival of sensory neurones. Nature. 1986 Feb 6;319(6053):497–499. [PubMed] [Google Scholar]
• Goedert M, Fine A, Hunt SP, Ullrich A. Nerve growth factor mRNA in peripheral and central rat tissues and in the human central nervous system: lesion effects in the rat brain and levels in Alzheimer's disease. Brain Res. 1986 Jul;387(1):85–92. [PubMed] [Google Scholar]
• Johnson JE, Barde YA, Schwab M, Thoenen H. Brain-derived neurotrophic factor supports the survival of cultured rat retinal ganglion cells. J Neurosci. 1986 Oct;6(10):3031–3038. [PMC free article] [PubMed] [Google Scholar]
• Edwards RH, Selby MJ, Rutter WJ. Differential RNA splicing predicts two distinct nerve growth factor precursors. Nature. 319(6056):784–787. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences