Adenosine triphosphate: nicotinamide mononucleotide adenylyltransferase of pig liver. Purification and properties - PubMed (original) (raw)
Adenosine triphosphate: nicotinamide mononucleotide adenylyltransferase of pig liver. Purification and properties
A M Ferro et al. Biochim Biophys Acta. 1975.
Abstract
Adenosine triphosphate : nicotinamide mononucleotide adenylyltransferase (EC 2.7.7.1) has been purifiec approximately 3500-fold from an extract of pig liver nuclei to a specific activity of 40 mumol of NAD+ per min per mg protein. The enzyme was found to have a molecular weight of 203 000, a frictional ratio of 1.6 and an isoelectric point of approximately 5. Michaelis constants for ATP and NMN were 0.11 mM and 0.12 mM, respectively.
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