PKC activation by melatonin modulates vimentin intermediate filament organization in N1E-115 cells - PubMed (original) (raw)

PKC activation by melatonin modulates vimentin intermediate filament organization in N1E-115 cells

G Benítez-King. J Pineal Res. 2000 Aug.

Abstract

Melatonin enters cells and causes cytoskeletal rearrangements in unicellular organisms, plants and vertebrates. This pineal secretory product causes microtubule enlargement and neurite outgrowth by a calmodulin antagonism in N1E-115 cells. Recently, direct in vitro activation of protein kinase C by melatonin was described. Vimentin intermediate filaments are attached to microtubules and their organization depends on both microtubule distribution and phosphorylation of specific proteins. Protein kinase C is a serine threonine kinase which phosphorylates vimentin and through this mechanism causes intermediate filament disassembly. In this work the effects of melatonin on protein kinase C activation, content, and subcellular distribution were studied in N1E-115 cells. Also, melatonin effects on vimentin phosphorylation and subcellular distribution were explored. The results show that melatonin both activates and increases protein kinase C content in the membrane cytoskeletal fraction. Melatonin protein kinase C activation was followed by an increase in both vimentin phosphorylation and by vimentin subcellular redistribution. Moreover, staurosporine, a serine threonine kinase inhibitor, prevented increased vimentin phosphorylation elicited by melatonin. Similar effects to those caused by melatonin were obtained with the protein kinase C activator phorbol 12-myristate 13-acetate. Data support the idea that melatonin modulates vimentin organization through protein kinase C activation.

PubMed Disclaimer

Similar articles

• Melatonin activates PKC-alpha but not PKC-epsilon in N1E-115 cells.
  Benítez-King G, Hernández ME, Tovar R, Ramírez G. Benítez-King G, et al. Neurochem Int. 2001 Aug;39(2):95-102. doi: 10.1016/s0197-0186(01)00021-3. Neurochem Int. 2001. PMID: 11408087
• Melatonin stimulates calmodulin phosphorylation by protein kinase C.
  Soto-Vega E, Meza I, Ramírez-Rodríguez G, Benitez-King G. Soto-Vega E, et al. J Pineal Res. 2004 Sep;37(2):98-106. doi: 10.1111/j.1600-079X.2004.00141.x. J Pineal Res. 2004. PMID: 15298668
• Melatonin induces neuritogenesis at early stages in N1E-115 cells through actin rearrangements via activation of protein kinase C and Rho-associated kinase.
  Bellon A, Ortíz-López L, Ramírez-Rodríguez G, Antón-Tay F, Benítez-King G. Bellon A, et al. J Pineal Res. 2007 Apr;42(3):214-21. doi: 10.1111/j.1600-079X.2006.00408.x. J Pineal Res. 2007. PMID: 17349018
• Melatonin as a cytoskeletal modulator: implications for cell physiology and disease.
  Benítez-King G. Benítez-King G. J Pineal Res. 2006 Jan;40(1):1-9. doi: 10.1111/j.1600-079X.2005.00282.x. J Pineal Res. 2006. PMID: 16313492 Review.
• Roles of microfilaments and intermediate filaments in adrenal steroidogenesis.
  Hall PF, Almahbobi G. Hall PF, et al. Microsc Res Tech. 1997 Mar 15;36(6):463-79. doi: 10.1002/(SICI)1097-0029(19970315)36:6<463::AID-JEMT4>3.0.CO;2-J. Microsc Res Tech. 1997. PMID: 9142693 Review.

Cited by

• Cleavage of p53-vimentin complex enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis of rheumatoid arthritis synovial fibroblasts.
  Yang X, Wang J, Liu C, Grizzle WE, Yu S, Zhang S, Barnes S, Koopman WJ, Mountz JD, Kimberly RP, Zhang HG. Yang X, et al. Am J Pathol. 2005 Sep;167(3):705-19. doi: 10.1016/S0002-9440(10)62045-7. Am J Pathol. 2005. PMID: 16127151 Free PMC article.
• Melatonin: neuritogenesis and neuroprotective effects in crustacean x-organ cells.
  Cary GA, Cuttler AS, Duda KA, Kusema ET, Myers JA, Tilden AR. Cary GA, et al. Comp Biochem Physiol A Mol Integr Physiol. 2012 Apr;161(4):355-60. doi: 10.1016/j.cbpa.2011.12.005. Epub 2011 Dec 16. Comp Biochem Physiol A Mol Integr Physiol. 2012. PMID: 22200560 Free PMC article.
• Cellular and biochemical actions of melatonin which protect against free radicals: role in neurodegenerative disorders.
  Ortiz GG, Benítez-King GA, Rosales-Corral SA, Pacheco-Moisés FP, Velázquez-Brizuela IE. Ortiz GG, et al. Curr Neuropharmacol. 2008 Sep;6(3):203-14. doi: 10.2174/157015908785777201. Curr Neuropharmacol. 2008. PMID: 19506721 Free PMC article.
• Melatonin decreases breast cancer metastasis by modulating Rho-associated kinase protein-1 expression.
  Borin TF, Arbab AS, Gelaleti GB, Ferreira LC, Moschetta MG, Jardim-Perassi BV, Iskander AS, Varma NR, Shankar A, Coimbra VB, Fabri VA, de Oliveira JG, Zuccari DA. Borin TF, et al. J Pineal Res. 2016 Jan;60(1):3-15. doi: 10.1111/jpi.12270. Epub 2015 Oct 20. J Pineal Res. 2016. PMID: 26292662 Free PMC article.
• Melatonin and Hippo Pathway: Is There Existing Cross-Talk?
  Lo Sardo F, Muti P, Blandino G, Strano S. Lo Sardo F, et al. Int J Mol Sci. 2017 Sep 6;18(9):1913. doi: 10.3390/ijms18091913. Int J Mol Sci. 2017. PMID: 28878191 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley