MHC class I ubiquitination by a viral PHD/LAP finger protein - PubMed (original) (raw)
MHC class I ubiquitination by a viral PHD/LAP finger protein
J M Boname et al. Immunity. 2001 Oct.
Free article
Abstract
The murine gamma-herpesvirus-68 K3 (MK3) is a PHD/LAP finger protein that downregulates major histocompatibility complex (MHC) class I expression. In transfected cell lines, MK3 was expressed in the endoplasmic reticulum (ER) membrane, where it bound the cytoplasmic tail of newly synthesized H-2D(b) glycoproteins and targeted them for degradation. Proteasome inhibitors blocked the degradation and led to an accumulation of ubiquitinated H-2D(b). Because this retained its native conformation, ubiquitination preceded any denaturation or dislocation to the cytosol. The PHD/LAP finger of MK3 was not required for H-2D(b) binding but was essential for its ubiquitination and degradation. Thus, gamma-herpesviruses have adapted the cellular PHD/LAP motif to immune evasion, apparently for the catalysis of MHC class I ubiquitination.
Similar articles
- Immune evasion by a novel family of viral PHD/LAP-finger proteins of gamma-2 herpesviruses and poxviruses.
Früh K, Bartee E, Gouveia K, Mansouri M. Früh K, et al. Virus Res. 2002 Sep;88(1-2):55-69. doi: 10.1016/s0168-1702(02)00120-x. Virus Res. 2002. PMID: 12297327 Review. - Ubiquitination is essential for human cytomegalovirus US11-mediated dislocation of MHC class I molecules from the endoplasmic reticulum to the cytosol.
Kikkert M, Hassink G, Barel M, Hirsch C, van der Wal FJ, Wiertz E. Kikkert M, et al. Biochem J. 2001 Sep 1;358(Pt 2):369-77. doi: 10.1042/0264-6021:3580369. Biochem J. 2001. PMID: 11513735 Free PMC article. - Requirements for the selective degradation of endoplasmic reticulum-resident major histocompatibility complex class I proteins by the viral immune evasion molecule mK3.
Wang X, Connors R, Harris MR, Hansen TH, Lybarger L. Wang X, et al. J Virol. 2005 Apr;79(7):4099-108. doi: 10.1128/JVI.79.7.4099-4108.2005. J Virol. 2005. PMID: 15767411 Free PMC article. - Endoplasmic reticulum-associated protein degradation.
Lord JM, Davey J, Frigerio L, Roberts LM. Lord JM, et al. Semin Cell Dev Biol. 2000 Jun;11(3):159-64. doi: 10.1006/scdb.2000.0160. Semin Cell Dev Biol. 2000. PMID: 10906272 Review.
Cited by
- MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells.
Villalón-Letelier F, Brooks AG, Londrigan SL, Reading PC. Villalón-Letelier F, et al. mBio. 2021 Oct 26;12(5):e0148421. doi: 10.1128/mBio.01484-21. Epub 2021 Sep 14. mBio. 2021. PMID: 34517760 Free PMC article. - Hepatitis C virus modulates signal peptide peptidase to alter host protein processing.
Hirano J, Yoshio S, Sakai Y, Songling L, Suzuki T, Itoh Y, Zhang H, Chen DV, Haga S, Oomori H, Kodama T, Maeda Y, Ono Y, Takahashi Y, Standley DM, Yamamoto M, Moriishi K, Moriya K, Kanto T, Takehara T, Koike K, Matsuura Y, Okamoto T. Hirano J, et al. Proc Natl Acad Sci U S A. 2021 Jun 1;118(22):e2026184118. doi: 10.1073/pnas.2026184118. Proc Natl Acad Sci U S A. 2021. PMID: 34035171 Free PMC article. - EBV miRNAs are potent effectors of tumor cell transcriptome remodeling in promoting immune escape.
Ungerleider N, Bullard W, Kara M, Wang X, Roberts C, Renne R, Tibbetts S, Flemington EK. Ungerleider N, et al. PLoS Pathog. 2021 May 6;17(5):e1009217. doi: 10.1371/journal.ppat.1009217. eCollection 2021 May. PLoS Pathog. 2021. PMID: 33956915 Free PMC article. - The endoplasmic reticulum unfolded protein response - homeostasis, cell death and evolution in virus infections.
Prasad V, Greber UF. Prasad V, et al. FEMS Microbiol Rev. 2021 Sep 8;45(5):fuab016. doi: 10.1093/femsre/fuab016. FEMS Microbiol Rev. 2021. PMID: 33765123 Free PMC article. Review. - Mechanism of Viral Glycoprotein Targeting by Membrane-Associated RING-CH Proteins.
Lun CM, Waheed AA, Majadly A, Powell N, Freed EO. Lun CM, et al. mBio. 2021 Mar 16;12(2):e00219-21. doi: 10.1128/mBio.00219-21. mBio. 2021. PMID: 33727347 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials