Presence of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells - PubMed (original) (raw)

Presence of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells

Xing-Wang Wang et al. World J Gastroenterol. 1998 Dec.

Abstract

AIM:To study the expression of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha-fetoprotein(AFP) and Fas/Bcl-2 proteins.METHODS:BEL-7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new-born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl-2 protein expression by the avidin-biotin complex (ABC) immunocytochemical assay.RESULTS: Immunocytochemical study showed that essentially all the BEL-7404 human hepatoma cells could express Fas and Bcl-2 proteins, although in various amount. No positive staining for Fas and Bcl-2 proteins was observed when cells were incubated with non-relevant sera, to establish the specificity.CONCLUSION:Fas apoptosis signals and Bcl-2 rescue/survival signals from apoptosis are expressed in BEL-7404 human hepatoma cells. The finding strongly implys that AFP-mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl-2 proteins present in those cells.

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Figures

Figure 1

Detection of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells. (1) Fas antigen was stained by the avidin- biotin-peroxidase complex. Reaction products were visible in almost all the cells. (2) Same as in 1, except that rabbit anti-human polyclonal antibody to Fas was replaced by normal rabbit serum. (3) BEL-7404 cells were incubated with mouse anti-human monoclonal antibody to Bcl-2. Positive grains detected by alkaline-phosphatase-conjugated avidin-biotin complex were also visible in almost all the cells. (4) Same as in 3, except that the primary antibody to Bcl-2 was replaced by normal mouse serum. Original magnification × 200.

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