Suramin inhibits the in vitro expression of encephalitis B virus proteins NS3 and E - PubMed (original) (raw)
Suramin inhibits the in vitro expression of encephalitis B virus proteins NS3 and E
Keshu Xu et al. J Huazhong Univ Sci Technolog Med Sci. 2003.
Abstract
In this study, the mechanism by which Suramin inhibits the replication of epidemic encephalitis B virus was explored to provide a theoretical basis for its further application in clinical practice. After viral infection of HepG2 and IMR-32 cells, different concentrations of Suramin were added to the culture media, and then the cultural supernatants and infected cells were collected 48 h later. For the evaluation of the curative effect, cytopathic effect (CPE), virus titers, the expression of viral protein and viral RNA were determined by Western blot, RT-PCR and in vitro RNA synthesis, respectively. At the concentration of 50 microg/ml of Suramin, HepG2 and IMR-32 infected with epidemic encephalitis B virus decreased by 51.8% and 0.03% respectively, as compared with controls. It was suggested that expression of encephalitis B virus proteins NS3 and E was notably reduced by Suramin. This is especially true of E protein. At RNA level, however, no difference in RNA virus was found between Suramin-treated virus and non-treated cells. Our results suggest that Suramin can inhibit viral replication by blocking the production of viral proteins.
Similar articles
- Inhibitory effect of furanonaphthoquinone derivatives on the replication of Japanese encephalitis virus.
Takegami T, Simamura E, Hirai K, Koyama J. Takegami T, et al. Antiviral Res. 1998 Jan;37(1):37-45. doi: 10.1016/s0166-3542(97)00058-2. Antiviral Res. 1998. PMID: 9497071 - The NS3 and NS4A genes as the targets of RNA interference inhibit replication of Japanese encephalitis virus in vitro and in vivo.
Yuan L, Wu R, Liu H, Wen X, Huang X, Wen Y, Ma X, Yan Q, Huang Y, Zhao Q, Cao S. Yuan L, et al. Gene. 2016 Dec 15;594(2):183-189. doi: 10.1016/j.gene.2016.08.055. Epub 2016 Sep 1. Gene. 2016. PMID: 27593564 - Degradation of MicroRNA miR-466d-3p by Japanese Encephalitis Virus NS3 Facilitates Viral Replication and Interleukin-1β Expression.
Jiang H, Bai L, Ji L, Bai Z, Su J, Qin T, Wang G, Balasubramaniam V, Wang X, Cui M, Ye J, Cao S, Li G, Yang Y. Jiang H, et al. J Virol. 2020 Jul 16;94(15):e00294-20. doi: 10.1128/JVI.00294-20. Print 2020 Jul 16. J Virol. 2020. PMID: 32461319 Free PMC article. - West Nile Virus NS2B/NS3 protease as an antiviral target.
Chappell KJ, Stoermer MJ, Fairlie DP, Young PR. Chappell KJ, et al. Curr Med Chem. 2008;15(27):2771-84. doi: 10.2174/092986708786242804. Curr Med Chem. 2008. PMID: 18991636 Review.
Cited by
- Screening and Evaluation of Novel Compounds against Hepatitis B Virus Polymerase Using Highly Purified Reverse Transcriptase Domain.
Ohsaki E, Ueda K. Ohsaki E, et al. Viruses. 2020 Jul 31;12(8):840. doi: 10.3390/v12080840. Viruses. 2020. PMID: 32752057 Free PMC article. - Suramin Inhibits Chikungunya Virus Entry and Transmission.
Ho YJ, Wang YM, Lu JW, Wu TY, Lin LI, Kuo SC, Lin CC. Ho YJ, et al. PLoS One. 2015 Jul 24;10(7):e0133511. doi: 10.1371/journal.pone.0133511. eCollection 2015. PLoS One. 2015. PMID: 26208101 Free PMC article. - Putative roles of purinergic signaling in human immunodeficiency virus-1 infection.
Pacheco PA, Faria RX, Ferreira LG, Paixão IC. Pacheco PA, et al. Biol Direct. 2014 Oct 29;9:21. doi: 10.1186/1745-6150-9-21. Biol Direct. 2014. PMID: 25351961 Free PMC article.
References
- Eur J Pharmacol. 1999 Mar 12;369(1):107-12 - PubMed
- Virus Res. 1989 Aug;13(4):337-50 - PubMed
- Biochemistry. 1992 Sep 22;31(37):9016-24 - PubMed
- Cancer Res. 1993 May 15;53(10 Suppl):2239-48 - PubMed
- Ann Pharm Fr. 2001 Feb;59(1):33-9 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials
Miscellaneous