A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytes - PubMed (original) (raw)
Comparative Study
. 2005 Mar;135(3):519-24.
doi: 10.1093/jn/135.3.519.
Affiliations
- PMID: 15735087
- DOI: 10.1093/jn/135.3.519
Free article
Comparative Study
A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytes
Kusum K Kharbanda et al. J Nutr. 2005 Mar.
Free article
Abstract
Previous studies showed that chronic ethanol administration alters methionine metabolism in the liver, resulting in increased intracellular S-adenosylhomocysteine (SAH) levels and increased homocysteine release into the plasma. We showed further that these changes appear to be reversed by betaine administration. This study compared the effects of betaine and S-adenosylmethionine (SAM), another methylating agent, on ethanol-induced changes of methionine metabolism and hepatic steatosis. Wistar rats were fed ethanol or control Lieber-Decarli liquid diet for 4 wk and metabolites of the methionine cycle were measured in isolated hepatocytes. Hepatocytes from ethanol-fed rats had a 50% lower intracellular SAM:SAH ratio and almost 2-fold greater homocysteine release into the media compared with controls. Supplementation of betaine or SAM in the incubation media increased this ratio in hepatocytes from both control and ethanol-fed rats and attenuated the ethanol-induced increased hepatocellular triglyceride levels by approximately 20%. On the other hand, only betaine prevented the increase in generation of homocysteine in the incubation media under basal and methionine-loaded conditions. SAM can correct only the ratio and the methylation defects and may in fact be detrimental after prolonged use because of its propensity to increase homocysteine release. Both SAM and betaine are effective in increasing the SAM:SAH ratio in hepatocytes and in attenuating hepatic steatosis; however, only betaine can effectively methylate homocysteine and prevent increased homocysteine release by the liver.
Similar articles
- Betaine lowers elevated s-adenosylhomocysteine levels in hepatocytes from ethanol-fed rats.
Barak AJ, Beckenhauer HC, Mailliard ME, Kharbanda KK, Tuma DJ. Barak AJ, et al. J Nutr. 2003 Sep;133(9):2845-8. doi: 10.1093/jn/133.9.2845. J Nutr. 2003. PMID: 12949375 - Betaine effects on hepatic methionine metabolism elicited by short-term ethanol feeding.
Barak AJ, Beckenhauer HC, Tuma DJ. Barak AJ, et al. Alcohol. 1996 Sep-Oct;13(5):483-6. doi: 10.1016/0741-8329(96)00040-7. Alcohol. 1996. PMID: 8888945 - Betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway.
Kharbanda KK, Mailliard ME, Baldwin CR, Beckenhauer HC, Sorrell MF, Tuma DJ. Kharbanda KK, et al. J Hepatol. 2007 Feb;46(2):314-21. doi: 10.1016/j.jhep.2006.08.024. Epub 2006 Oct 26. J Hepatol. 2007. PMID: 17156888 - Alcoholic liver disease and methionine metabolism.
Kharbanda KK. Kharbanda KK. Semin Liver Dis. 2009 May;29(2):155-65. doi: 10.1055/s-0029-1214371. Epub 2009 Apr 22. Semin Liver Dis. 2009. PMID: 19387915 Review. - Betaine, ethanol, and the liver: a review.
Barak AJ, Beckenhauer HC, Tuma DJ. Barak AJ, et al. Alcohol. 1996 Jul-Aug;13(4):395-8. doi: 10.1016/0741-8329(96)00030-4. Alcohol. 1996. PMID: 8836329 Review.
Cited by
- Role of Nutrition in Alcoholic Liver Disease: Summary of the Symposium at the ESBRA 2017 Congress.
Kharbanda KK, Ronis MJJ, Shearn CT, Petersen DR, Zakhari S, Warner DR, Feldstein AE, McClain CJ, Kirpich IA. Kharbanda KK, et al. Biomolecules. 2018 Mar 26;8(2):16. doi: 10.3390/biom8020016. Biomolecules. 2018. PMID: 29587455 Free PMC article. - Acute alcohol exposure during mouse gastrulation alters lipid metabolism in placental and heart development: Folate prevention.
Linask KK, Han M. Linask KK, et al. Birth Defects Res A Clin Mol Teratol. 2016 Sep;106(9):749-60. doi: 10.1002/bdra.23526. Epub 2016 Jun 14. Birth Defects Res A Clin Mol Teratol. 2016. PMID: 27296863 Free PMC article. - Effects of S-adenosylmethionine on liver methionine metabolism and steatosis with ethanol-induced liver injury in rats.
Gong Z, Yan S, Zhang P, Huang Y, Wang L. Gong Z, et al. Hepatol Int. 2008 Sep;2(3):346-52. doi: 10.1007/s12072-008-9082-1. Epub 2008 Jul 25. Hepatol Int. 2008. PMID: 19669264 Free PMC article. - Hepatoprotective effects of S-adenosyl-L-methionine against alcohol- and cytochrome P450 2E1-induced liver injury.
Cederbaum AI. Cederbaum AI. World J Gastroenterol. 2010 Mar 21;16(11):1366-76. doi: 10.3748/wjg.v16.i11.1366. World J Gastroenterol. 2010. PMID: 20238404 Free PMC article. Review. - Assessment of the effect of betaine on p16 and c-myc DNA methylation and mRNA expression in a chemical induced rat liver cancer model.
Du YP, Peng JS, Sun A, Tang ZH, Ling WH, Zhu HL. Du YP, et al. BMC Cancer. 2009 Jul 30;9:261. doi: 10.1186/1471-2407-9-261. BMC Cancer. 2009. PMID: 19642983 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources