Proteome profiling of Populus euphratica Oliv. upon heat stress - PubMed (original) (raw)

Proteome profiling of Populus euphratica Oliv. upon heat stress

Sílvia Ferreira et al. Ann Bot. 2006 Aug.

Abstract

Background and aims: Populus euphratica is a light-demanding species ecologically characterized as a pioneer. It grows in shelter belts along riversides, being part of the natural desert forest ecosystems in China and Middle Eastern countries. It is able to survive extreme temperatures, drought and salt stress, marking itself out as an important plant species to study the mechanisms responsible for survival of woody plants under heat stress.

Methods: Heat effects were evaluated through electrolyte leakage on leaf discs, and LT(50) was determined to occur above 50 degrees C. Protein accumulation profiles of leaves from young plants submitted to 42/37 degrees C for 3 d in a phytotron were determined through 2D-PAGE, and a total of 45 % of up- and downregulated proteins were detected. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)/TOF analysis, combined with searches in different databases, enabled the identification of 82 % of the selected spots.

Key results: Short-term upregulated proteins are related to membrane destabilization and cytoskeleton restructuring, sulfur assimilation, thiamine and hydrophobic amino acid biosynthesis, and protein stability. Long-term upregulated proteins are involved in redox homeostasis and photosynthesis. Late downregulated proteins are involved mainly in carbon metabolism.

Conclusions: Moderate heat response involves proteins related to lipid biogenesis, cytoskeleton structure, sulfate assimilation, thiamine and hydrophobic amino acid biosynthesis, and nuclear transport. Photostasis is achieved through carbon metabolism adjustment, a decrease of photosystem II (PSII) abundance and an increase of PSI contribution to photosynthetic linear electron flow. Thioredoxin h may have a special role in this process in P. euphratica upon moderate heat exposure.

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Figures

F<sc>ig</sc>. 1.

Fig. 1.

Percentage of membrane damage of leaf cells from plants of P. euphratica submitted to temperatures up to 55 °C. Columns represent the average percentage membrane damage, calculated from the results of three independent experiments. The _y_-axis represents the total percentage of electrolyte leakage and the _x_-axis represents the temperature, in °C, to which leaf discs were exposed.

F<sc>ig</sc>. 2

Fig. 2

Position of identified spots from 2D 12·5 % polyacrylamide gels. From left to right, pI 4–7 linear range. Arrowheads indicate the spots identified by mass spectrometry, together with the respective spot number presented in Table 2.

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