Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi - PubMed (original) (raw)

Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi

Lisa A Fitzgerald et al. Virology. 2007.

Abstract

Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of the viral gene products resemble entries in public databases, including some that are the first of their kind to be detected in a virus. For example, these unique gene products include an aquaglyceroporin in MT325, a potassium ion transporter protein and an alkyl sulfatase in FR483, and a dTDP-glucose pyrophosphorylase in both viruses. Comparison of MT325 and FR483 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that approximately 82% of the genes are present in all three viruses.

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Figures

Fig. 1

Fig. 1

Comparison of three sequenced chlorella viruses, PBCV-1, MT325, and FR483, major ORFs with blastp dot plots. The dots represent ORF homology between two viruses with an E-value of less than 0.001.

Fig. 2

Fig. 2

General characteristics of MT325 and FR483 major ORFs. Direction of the MT325 (A) and FR483 (B) ORFs. Size of the major MT325 (C) and FR483 (D) ORFs. Predicted isoelectric points of the major MT325 (E) and FR483 (F) ORFs. Intergenic space between major MT325 (G) and FR483 (H) ORFs.

Fig. 3

Fig. 3

Map of the MT325 and FR483 genomes. The predicted open reading frames are shown in both orientations and colored according to their functional category. The inner green circle represents the percent A+T across the genome using a 25 bp window.

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