Construction and characterization of single-chain antibodies against human insulin-like growth factor-I receptor from hybridomas producing 1H7 or 3B7 monoclonal antibody - PubMed (original) (raw)
doi: 10.1093/jb/mvm192. Epub 2007 Oct 23.
Affiliations
- PMID: 17956902
- DOI: 10.1093/jb/mvm192
Construction and characterization of single-chain antibodies against human insulin-like growth factor-I receptor from hybridomas producing 1H7 or 3B7 monoclonal antibody
Yu Kusada et al. J Biochem. 2008 Jan.
Abstract
Recombinant antibody consisting of the single-chain variable fragment (scFv) of 1H7 monoclonal antibody against insulin-like growth factor-I receptor (IGF-IR) and human IgG(1) Fc domain, scFv-Fc, has been found to exhibit inhibitory effects on breast cancer growth in vitro and in vivo [Li et al. (2000) Cancer Immunol. Immunother. 49, 243; Sachdev et al. (2003) Cancer Res. 63, 627]. Various types of scFvs from hybridomas producing 1H7 or 3B7 mAb were constructed using conventional phage display technology to further characterize the specificity and affinity of anti-IGF-IR mAbs. Binding studies performed using either phage antibodies or soluble scFv proteins to IGF-IR or insulin receptor (IR) and IGF-IR pre-incubated with mAbs suggested that (i) 1H7 and 3B7 bind to IGF-IR but do not bind to its structurally related IR, (ii) either the VL-VH or VH-VL sequence order does not apparently affect specificity for IGF-IR and (iii) 1H7 and 3B7 bind the independent epitopes, located in or near the N-terminal (440-514) and C-terminal (62-184) domains of the alpha subunit, respectively. This study not only revealed new information on binding regions for two anti-IGF-IR mAbs, but also provided the scFv genes as tools for further manipulation of the affinity or development of new IGF-IR-targeted cancer therapeutics.
Similar articles
- Single-chain antibodies against human insulin-like growth factor I receptor: expression, purification, and effect on tumor growth.
Li SL, Liang SJ, Guo N, Wu AM, Fujita-Yamaguchi Y. Li SL, et al. Cancer Immunol Immunother. 2000 Jul;49(4-5):243-52. doi: 10.1007/s002620000115. Cancer Immunol Immunother. 2000. PMID: 10941907 Free PMC article. - Combined effects of tamoxifen and a chimeric humanized single chain antibody against the type I IGF receptor on breast tumor growth in vivo.
Ye JJ, Liang SJ, Guo N, Li SL, Wu AM, Giannini S, Sachdev D, Yee D, Brünner N, Ikle D, Fujita-Yamaguchi Y. Ye JJ, et al. Horm Metab Res. 2003 Nov-Dec;35(11-12):836-42. doi: 10.1055/s-2004-814145. Horm Metab Res. 2003. PMID: 14710366 - Mechanisms of antibody-mediated insulin-like growth factor I receptor (IGF-IR) down-regulation in MCF-7 breast cancer cells.
Ohtani M, Numazaki M, Yajima Y, Fujita-Yamaguchi Y. Ohtani M, et al. Biosci Trends. 2009 Aug;3(4):131-8. Biosci Trends. 2009. PMID: 20103837 - IMC-A12, a human IgG1 monoclonal antibody to the insulin-like growth factor I receptor.
Rowinsky EK, Youssoufian H, Tonra JR, Solomon P, Burtrum D, Ludwig DL. Rowinsky EK, et al. Clin Cancer Res. 2007 Sep 15;13(18 Pt 2):5549s-5555s. doi: 10.1158/1078-0432.CCR-07-1109. Clin Cancer Res. 2007. PMID: 17875788 Review. - Insulin receptor structure and its implications for the IGF-1 receptor.
Lawrence MC, McKern NM, Ward CW. Lawrence MC, et al. Curr Opin Struct Biol. 2007 Dec;17(6):699-705. doi: 10.1016/j.sbi.2007.07.007. Epub 2007 Sep 11. Curr Opin Struct Biol. 2007. PMID: 17851071 Review.
Cited by
- IGF1R- and ROR1-Specific CAR T Cells as a Potential Therapy for High Risk Sarcomas.
Huang X, Park H, Greene J, Pao J, Mulvey E, Zhou SX, Albert CM, Moy F, Sachdev D, Yee D, Rader C, Hamby CV, Loeb DM, Cairo MS, Zhou X. Huang X, et al. PLoS One. 2015 Jul 14;10(7):e0133152. doi: 10.1371/journal.pone.0133152. eCollection 2015. PLoS One. 2015. PMID: 26173023 Free PMC article. - A stimulatory thyrotropin receptor antibody enhances hyaluronic acid synthesis in graves' orbital fibroblasts: inhibition by an IGF-I receptor blocking antibody.
Kumar S, Iyer S, Bauer H, Coenen M, Bahn RS. Kumar S, et al. J Clin Endocrinol Metab. 2012 May;97(5):1681-7. doi: 10.1210/jc.2011-2890. Epub 2012 Mar 7. J Clin Endocrinol Metab. 2012. PMID: 22399503 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources