Isomerization and/or racemization at Asp23 of Abeta42 do not increase its aggregative ability, neurotoxicity, and radical productivity in vitro - PubMed (original) (raw)

. 2008 Feb 15;366(3):745-51.

doi: 10.1016/j.bbrc.2007.12.009. Epub 2007 Dec 17.

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Isomerization and/or racemization at Asp23 of Abeta42 do not increase its aggregative ability, neurotoxicity, and radical productivity in vitro

Kazuma Murakami et al. Biochem Biophys Res Commun. 2008.

Abstract

Aggregation of the 42-mer amyloid beta peptide (Abeta42) plays a pivotal role in the pathogenesis of Alzheimer's disease. Recent investigations suggested the isomerization and/or racemization of Asp at position 1, 7, or 23 to be associated with the pathological role of Abeta42. Our previous study indicated that the turn at positions 22 and 23 of Abeta42 is closely related to its neurotoxicity through the formation of radicals. To clarify the contribution of these modifications at Asp23 to the pathology, three isomerized and/or racemized Abeta42 mutants were prepared. l-isoAsp23- and d-Asp23-Abeta42 showed moderate aggregative ability similar to the wild type. However, d-Asp23-Abeta42 was less neurotoxic than the wild type, while l-isoAsp23-Abeta42 was as toxic as the wild type. In contrast, d-isoAsp23-Abeta42 showed weak aggregative ability without neurotoxicity. These results suggest the isomerization and/or racemization of Asp23 not to be related to the pathogenesis, but to be a consequence of chemical reactions during the long-term deposition of fibrils.

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