Removal of free extracellular DNA from environmental samples by ethidium monoazide and propidium monoazide - PubMed (original) (raw)

Removal of free extracellular DNA from environmental samples by ethidium monoazide and propidium monoazide

Andreas O Wagner et al. Appl Environ Microbiol. 2008 Apr.

Abstract

Recently, new DNA extraction techniques (using ethidium monoazide and propidium monoazide) have been developed to discriminate between alive and dead bacterial cells. Nevertheless, for complex environmental samples, no data are available yet. In the present study, these new methods were applied to anaerobic-fermentor sludge and the results were compared to a conventional microbiological approach.

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Figures

FIG. 1.

FIG. 1.

Amounts of total extracted DNA (PicoGreen measurement) as a result of different extraction procedures. Significant differences (P < 0.05) are symbolized by different letters. For an explanation, refer to the text.

FIG. 2.

FIG. 2.

Survival of heat-treated cells of S. enterica in DFS. The results of a comparison of different DNA extraction procedures (conventional, EMA, 5 μM PMA, and 50 μM PMA) with subsequent qPCR and a classical microbiological approach (CFU) are shown.

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