Expression of cripto, a novel gene of the epidermal growth factor gene family, leads to in vitro transformation of a normal mouse mammary epithelial cell line - PubMed (original) (raw)
. 1991 Feb 1;51(3):1051-4.
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- PMID: 1846316
Expression of cripto, a novel gene of the epidermal growth factor gene family, leads to in vitro transformation of a normal mouse mammary epithelial cell line
F Ciardiello et al. Cancer Res. 1991.
Abstract
cripto is a gene encoding an epidermal growth factor-related protein that is expressed in undifferentiated embryonal carcinoma cells. To ascertain if cripto is capable of functioning as a transforming gene, a full-length human cripto complementary DNA under the transcriptional control of the Rous sarcoma virus long terminal repeat has been cotransfected with the selectable pSV2neo marker plasmid into immortalized mouse NOG-8 mammary epithelial cells. Several neomycin-resistant clones were isolated that express high levels of a specific cripto 4.5-kilobase mRNA transcript and possess multiple copies of cripto plasmid DNA. NOG-8 cells that express cripto are able to clone in soft agar and exhibit an approximately 3-fold increase in their anchorage-dependent growth in serum-free medium as compared to the neo-transfected NOG-8 cells. However, none of the cripto-expressing NOG-8 clones are able to form tumors in nude mice.
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