The secondary metabolite euplotin C induces apoptosis-like death in the marine ciliated protist Euplotes vannus - PubMed (original) (raw)
The secondary metabolite euplotin C induces apoptosis-like death in the marine ciliated protist Euplotes vannus
Davide Cervia et al. J Eukaryot Microbiol. 2009 May-Jun.
Free article
Abstract
The sesquiterpenoid euplotin C is a secondary metabolite produced by the ciliated protist Euplotes crassus and provides a mechanism for damping populations of potential competitors. Indeed, E. crassus is virtually resistant to its own product while different non-producer species representing an unbiased sample of the marine, interstitial, ciliate diversity are sensitive. For instance, euplotin C exerts a marked disruption of different homeostatic mechanisms in Euplotes vannus. We demonstrate by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay that euplotin C quickly decreases viability and mitochondrial function of E. vannus with a very high efficacy and at micromolar potency. In addition, euplotin C induces apoptosis in E. vannus as 4,6-diamino-2-phenylindole and terminal transferase dUTP nick end labeling staining show the rapid condensation and fragmentation of nuclear material in cells treated with euplotin C. These effects occur without detectable permeabilisation or rupture of cell membranes and with no major changes in the overall morphology, although some traits, such as vacuolisation and disorganized microtubules, can be observed by transmission electron microscopy. In particular, E. vannus show profound changes of the mitochondrial ultrastructure. Finally, we also show that caspase activity in E. vannus is increased by euplotin C. These data elucidate the pro-apoptotic role of euplotin C and suggest a mechanism for its impact on natural selection.
Similar articles
- Anticancer Activity of Euplotin C, Isolated from the Marine Ciliate Euplotes crassus, Against Human Melanoma Cells.
Carpi S, Polini B, Poli G, Alcantara Barata G, Fogli S, Romanini A, Tuccinardi T, Guella G, Frontini FP, Nieri P, Di Giuseppe G. Carpi S, et al. Mar Drugs. 2018 May 16;16(5):166. doi: 10.3390/md16050166. Mar Drugs. 2018. PMID: 29772645 Free PMC article. - Action mechanisms of the secondary metabolite euplotin C: signaling and functional role in Euplotes.
Trielli F, Cervia D, Di Giuseppe G, Ristori C, Kruppel T, Burlando B, Guella G, Viarengo A, Bagnoli P, Delmonte Corrado MU, Dini F. Trielli F, et al. J Eukaryot Microbiol. 2008 Sep-Oct;55(5):365-73. doi: 10.1111/j.1550-7408.2008.00335.x. J Eukaryot Microbiol. 2008. PMID: 19017056 - Cytotoxic effects and apoptotic signalling mechanisms of the sesquiterpenoid euplotin C, a secondary metabolite of the marine ciliate Euplotes crassus, in tumour cells.
Cervia D, Martini D, Garcia-Gil M, Di Giuseppe G, Guella G, Dini F, Bagnoli P. Cervia D, et al. Apoptosis. 2006 May;11(5):829-43. doi: 10.1007/s10495-006-5700-3. Apoptosis. 2006. PMID: 16534550 - Antimicrobial activity of euplotin C, the sesquiterpene taxonomic marker from the marine ciliate Euplotes crassus.
Savoia D, Avanzini C, Allice T, Callone E, Guella G, Dini F. Savoia D, et al. Antimicrob Agents Chemother. 2004 Oct;48(10):3828-33. doi: 10.1128/AAC.48.10.3828-3833.2004. Antimicrob Agents Chemother. 2004. PMID: 15388442 Free PMC article. - Evaluation of the sensitivity to zinc of ciliates Euplotes vannus and Euplotes crassus and their naturally associated bacteria isolated from a polluted tropical bay.
Bitencourt JA, Pereira DC, da Silva Neto ID, Crapez MA. Bitencourt JA, et al. Environ Sci Pollut Res Int. 2015 Apr;22(8):6236-45. doi: 10.1007/s11356-014-3828-1. Epub 2014 Nov 20. Environ Sci Pollut Res Int. 2015. PMID: 25408072
Cited by
- Complexing the Marine Sesquiterpene Euplotin C by Means of Cyclodextrin-Based Nanosponges: A Preliminary Investigation.
Bertoli A, LoBue A, Quattrini L, Sartini S, Polini B, Carpi S, Frontini FP, Di Giuseppe G, Guella G, Nieri P, La Motta C. Bertoli A, et al. Mar Drugs. 2022 Oct 29;20(11):682. doi: 10.3390/md20110682. Mar Drugs. 2022. PMID: 36355005 Free PMC article. - Divergences in the response to ultraviolet radiation between polar and non-polar ciliated protozoa: UV radiation effects in Euplotes.
Di Giuseppe G, Cervia D, Vallesi A. Di Giuseppe G, et al. Microb Ecol. 2012 Feb;63(2):334-8. doi: 10.1007/s00248-011-9934-4. Epub 2011 Sep 9. Microb Ecol. 2012. PMID: 21904954 - Bioactivity and Structural Properties of Novel Synthetic Analogues of the Protozoan Toxin Climacostol.
Buonanno F, Catalani E, Cervia D, Proietti Serafini F, Picchietti S, Fausto AM, Giorgi S, Lupidi G, Rossi FV, Marcantoni E, Petrelli D, Ortenzi C. Buonanno F, et al. Toxins (Basel). 2019 Jan 15;11(1):42. doi: 10.3390/toxins11010042. Toxins (Basel). 2019. PMID: 30650514 Free PMC article. - Anticancer Activity of Euplotin C, Isolated from the Marine Ciliate Euplotes crassus, Against Human Melanoma Cells.
Carpi S, Polini B, Poli G, Alcantara Barata G, Fogli S, Romanini A, Tuccinardi T, Guella G, Frontini FP, Nieri P, Di Giuseppe G. Carpi S, et al. Mar Drugs. 2018 May 16;16(5):166. doi: 10.3390/md16050166. Mar Drugs. 2018. PMID: 29772645 Free PMC article.