MicroRNA-193b regulates proliferation, migration and invasion in human hepatocellular carcinoma cells - PubMed (original) (raw)

. 2010 Oct;46(15):2828-36.

doi: 10.1016/j.ejca.2010.06.127. Epub 2010 Jul 23.

Affiliations

• PMID: 20655737
• DOI: 10.1016/j.ejca.2010.06.127

MicroRNA-193b regulates proliferation, migration and invasion in human hepatocellular carcinoma cells

Chengwang Xu et al. Eur J Cancer. 2010 Oct.

Abstract

Background and aims: Recently, some miRNAs have been reported to be connected closely with the development of human hepatocellular carcinoma. However, the functions of these miRNAs in HCC remain largely undefined.

Methods: The expression profiles of miR-193b were compared between HCC tissues and adjacent normal liver tissues using qRT-PCR method. This method was also be used to screen the potential target genes of miR-193b. A luciferase reporter assay was conducted to confirm target association. Finally, the functional effect of miR-193b in hepatoma cells was examined further.

Results: miR-193b was significantly down-regulated in most of the HCC tissues compared to the matching non-tumoural liver tissues. Furthermore, ectopic expression of miR-193b dramatically suppressed the ability of hepatoma cells to form colonies in vitro and to develop tumours in nude mice. CCND1 and ETS1 were revealed to be regulated by miR-193b directly. By regulating the expressions of these oncogenes, miR-193b induced cell cycle arrest and inhibited the invasion and migration of hepatoma cells.

Conclusions: miR-193b may function as a tumour suppressor in the development of HCC by acting on multiple tumourigenic pathways.

Copyright © 2010 Elsevier Ltd. All rights reserved.

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