Biodiesel production from pomace oil by using lipase immobilized onto olive pomace - PubMed (original) (raw)
Biodiesel production from pomace oil by using lipase immobilized onto olive pomace
Yasin Yücel. Bioresour Technol. 2011 Feb.
Abstract
In the present work, microbial lipase from Thermomyces lanuginosus was immobilized by covalent binding onto olive pomace. Immobilized support material used to produce biodiesel with pomace oil and methanol. The properties of the support and immobilized derivative were evaluated by scanning electron microscopy (SEM). The maximum immobilization of T. lanuginosus was obtained as 18.67 mg/g support and the highest specific activity was 10.31 U/mg protein. The properties of immobilized lipase were studied. The effects of protein concentration, pH and buffer concentration on the immobilization and lipase activity were investigated. Biodiesel production using the immobilized lipase was realized by a three-step addition of methanol to avoid strong substrate inhibition. Under the optimized conditions, the maximum biodiesel yield was 93% at 25°C in 24h reaction. The immobilized enzyme retained its activity during the 10 repeated batch reactions.
Copyright © 2010 Elsevier Ltd. All rights reserved.
Similar articles
- Covalent attachment of microbial lipase onto microporous styrene-divinylbenzene copolymer by means of polyglutaraldehyde.
Dizge N, Keskinler B, Tanriseven A. Dizge N, et al. Colloids Surf B Biointerfaces. 2008 Oct 1;66(1):34-8. doi: 10.1016/j.colsurfb.2008.05.003. Epub 2008 May 15. Colloids Surf B Biointerfaces. 2008. PMID: 18571389 - Biodiesel fuel production by the transesterification reaction of soybean oil using immobilized lipase.
Bernardes OL, Bevilaqua JV, Leal MC, Freire DM, Langone MA. Bernardes OL, et al. Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):105-14. doi: 10.1007/s12010-007-9043-5. Appl Biochem Biotechnol. 2007. PMID: 18478380 - Biodiesel production from sunflower, soybean, and waste cooking oils by transesterification using lipase immobilized onto a novel microporous polymer.
Dizge N, Aydiner C, Imer DY, Bayramoglu M, Tanriseven A, Keskinler B. Dizge N, et al. Bioresour Technol. 2009 Mar;100(6):1983-91. doi: 10.1016/j.biortech.2008.10.008. Epub 2008 Nov 22. Bioresour Technol. 2009. PMID: 19028094 - [Progress on biodiesel production with enzymatic catalysis in China].
Tan T, Lu J, Nie K, Zhang H, Deng L, Wang F. Tan T, et al. Sheng Wu Gong Cheng Xue Bao. 2010 Jul;26(7):903-6. Sheng Wu Gong Cheng Xue Bao. 2010. PMID: 20954390 Review. Chinese. - Lipases as biocatalyst for biodiesel production.
Fan X, Niehus X, Sandoval G. Fan X, et al. Methods Mol Biol. 2012;861:471-83. doi: 10.1007/978-1-61779-600-5_27. Methods Mol Biol. 2012. PMID: 22426735 Review.
Cited by
- In silico design of multipoint mutants for enhanced performance of Thermomyces lanuginosus lipase for efficient biodiesel production.
Huang J, Xie X, Zheng W, Xu L, Yan J, Wu Y, Yang M, Yan Y. Huang J, et al. Biotechnol Biofuels Bioprod. 2024 Feb 24;17(1):33. doi: 10.1186/s13068-024-02478-5. Biotechnol Biofuels Bioprod. 2024. PMID: 38402206 Free PMC article. - Biodiesel Production by Single and Mixed Immobilized Lipases Using Waste Cooking Oil.
Ben Bacha A, Alonazi M, Alharbi MG, Horchani H, Ben Abdelmalek I. Ben Bacha A, et al. Molecules. 2022 Dec 9;27(24):8736. doi: 10.3390/molecules27248736. Molecules. 2022. PMID: 36557867 Free PMC article. - Waste Management in the Agri-Food Industry: The Conversion of Eggshells, Spent Coffee Grounds, and Brown Onion Skins into Carriers for Lipase Immobilization.
Budžaki S, Velić N, Ostojčić M, Stjepanović M, Rajs BB, Šereš Z, Maravić N, Stanojev J, Hessel V, Strelec I. Budžaki S, et al. Foods. 2022 Jan 30;11(3):409. doi: 10.3390/foods11030409. Foods. 2022. PMID: 35159559 Free PMC article. Review. - An encapsulated report on enzyme-assisted transesterification with an allusion to lipase.
Sundaramahalingam MA, Amrutha C, Sivashanmugam P, Rajeshbanu J. Sundaramahalingam MA, et al. 3 Biotech. 2021 Nov;11(11):481. doi: 10.1007/s13205-021-03003-3. Epub 2021 Oct 30. 3 Biotech. 2021. PMID: 34790505 Free PMC article. Review. - Production of biodiesel by enzymatic transesterification of waste sardine oil and evaluation of its engine performance.
Arumugam A, Ponnusami V. Arumugam A, et al. Heliyon. 2017 Dec 28;3(12):e00486. doi: 10.1016/j.heliyon.2017.e00486. eCollection 2017 Dec. Heliyon. 2017. PMID: 29322108 Free PMC article.