The proto-oncogene int-1 encodes a secreted protein associated with the extracellular matrix - PubMed (original) (raw)
The proto-oncogene int-1 encodes a secreted protein associated with the extracellular matrix
R S Bradley et al. EMBO J. 1990 May.
Abstract
The proto-oncogene int-1 plays an important role in mammary tumorigenesis when activated by proviral insertions of the mouse mammary tumor virus. In normal mouse tissues the gene is expressed in the embryonic neural tube, suggesting a developmental function, while in Drosophila the homolog of int-1 is the segment polarity gene wingless. In order to study the protein products of int-1 we have derived fibroblast cell lines infected with multiple copies of a retroviral vector expressing int-1 cDNA. By Western blot analysis and immunoprecipitation we have identified a 44 kd form of int-1 protein which is secreted from these cells. The 44 kd species is distinct from the major intracellular forms of int-1 protein as judged by its slower mobility in SDS-polyacrylamide gels and by its longer half-life in pulse-chase experiments. Under normal growth conditions, little or none of the 44 kd protein is detectable in the cell culture medium but instead the majority is found associated with the extracellular matrix (ECM). The protein appears to bind heparin in vitro, suggesting that it might bind glycosaminoglycans in the ECM. These data support the view that int-1 protein may play a role in cell-cell communication over short distances.
Similar articles
- Inducible overexpression and secretion of int-1 protein.
Papkoff J. Papkoff J. Mol Cell Biol. 1989 Aug;9(8):3377-84. doi: 10.1128/mcb.9.8.3377-3384.1989. Mol Cell Biol. 1989. PMID: 2677670 Free PMC article. - int-1--a proto-oncogene involved in cell signalling.
McMahon AP, Moon RT. McMahon AP, et al. Development. 1989;107 Suppl:161-7. doi: 10.1242/dev.107.Supplement.161. Development. 1989. PMID: 2534596 Review. - Wnt-1 and int-2 mammary oncogene effects on the beta-catenin pathway in immortalized mouse mammary epithelial cells are not sufficient for tumorigenesis.
Hollmann CA, Kittrell FS, Medina D, Butel JS. Hollmann CA, et al. Oncogene. 2001 Nov 15;20(52):7645-57. doi: 10.1038/sj.onc.1204980. Oncogene. 2001. PMID: 11753642 - The int genes in mouse mammary tumorigenesis and in normal development.
Nusse R. Nusse R. Ciba Found Symp. 1990;150:212-22; discussion 222-6. doi: 10.1002/9780470513927.ch13. Ciba Found Symp. 1990. PMID: 2164908 Review. - Secreted int-1 protein is associated with the cell surface.
Papkoff J, Schryver B. Papkoff J, et al. Mol Cell Biol. 1990 Jun;10(6):2723-30. doi: 10.1128/mcb.10.6.2723-2730.1990. Mol Cell Biol. 1990. PMID: 2140430 Free PMC article.
Cited by
- Regulation of Wnt distribution and function by Drosophila glypicans.
Waghmare I, Page-McCaw A. Waghmare I, et al. J Cell Sci. 2022 Feb 1;135(3):jcs259405. doi: 10.1242/jcs.259405. Epub 2022 Feb 3. J Cell Sci. 2022. PMID: 35112708 Free PMC article. Review. - Mesenchymal Cell-Derived Juxtacrine Wnt1 Signaling Regulates Osteoblast Activity and Osteoclast Differentiation.
Wang F, Tarkkonen K, Nieminen-Pihala V, Nagano K, Majidi RA, Puolakkainen T, Rummukainen P, Lehto J, Roivainen A, Zhang FP, Mäkitie O, Baron R, Kiviranta R. Wang F, et al. J Bone Miner Res. 2019 Jun;34(6):1129-1142. doi: 10.1002/jbmr.3680. Epub 2019 Mar 7. J Bone Miner Res. 2019. PMID: 30690791 Free PMC article. - Mechanisms of Wnt signaling and control.
Grainger S, Willert K. Grainger S, et al. Wiley Interdiscip Rev Syst Biol Med. 2018 Sep;10(5):e1422. doi: 10.1002/wsbm.1422. Epub 2018 Mar 30. Wiley Interdiscip Rev Syst Biol Med. 2018. PMID: 29600540 Free PMC article. Review. - WNT signaling - lung cancer is no exception.
Rapp J, Jaromi L, Kvell K, Miskei G, Pongracz JE. Rapp J, et al. Respir Res. 2017 Sep 5;18(1):167. doi: 10.1186/s12931-017-0650-6. Respir Res. 2017. PMID: 28870231 Free PMC article. Review. - Wnt Signaling in Cell Motility and Invasion: Drawing Parallels between Development and Cancer.
Sedgwick AE, D'Souza-Schorey C. Sedgwick AE, et al. Cancers (Basel). 2016 Aug 29;8(9):80. doi: 10.3390/cancers8090080. Cancers (Basel). 2016. PMID: 27589803 Free PMC article. Review.
References
- Nature. 1987 Apr 30-May 6;326(6116):833 - PubMed
- Cell. 1987 Aug 14;50(4):649-57 - PubMed
- Cell. 1982 Nov;31(1):99-109 - PubMed
- Mol Cell Biol. 1985 Dec;5(12):3337-44 - PubMed
- Mol Cell Biol. 1989 Nov;9(11):4896-902 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources