Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma - PubMed (original) (raw)

doi: 10.1002/hed.22959. Epub 2012 Feb 24.

Affiliations

PMID: 22367676
DOI: 10.1002/hed.22959

Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma

Yin-Hua Shih et al. Head Neck. 2013 Feb.

Abstract

Background: Lysyl oxidase (LOX) is a copper-dependent enzyme that cross-links collagen and elastin in the extracellular matrix. LOX overexpressed in various tumors. The manner in which LOX affects tumor growth remains controversial.

Methods: Chemical treatment and gene transfection were used to induce LOX overexpression or inhibition in cell lines SAS and SVEC4-10. LOX mRNA, protein, and activity were confirmed before tube formation assay and tumorigenesis. The microvessels in the tumor section were detected by immunostaining CD31-positive endothelial cells.

Results: LOX overexpression and copper induction of LOX activity increased SVEC4-10 tube formation. LOX silencing and β-aminopropionitrile inhibition of LOX activity had opposite effects. LOX overexpression increased proliferation and proliferating cell nuclear antigen expression. High LOX expression clones increased tumor size in a tumorigenesis model. The microvascular numbers were higher in LOX overexpression tumors than in control tumors.

Conclusion: LOX can induce cell proliferation and angiogenesis in oral squamous cell carcinoma.

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Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma - PubMed (original) (raw)