Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein - PubMed (original) (raw)

Fig 2

Structural homology between CCHFV and LASV N proteins and phylogenetic analysis of segmented negative-strand RNA viruses by N and L protein comparison. (A) Structural alignment of CCHFV and LASV N protein structures. The CCHFV protein is shown in aquamarine. Most of the LASV N structure is shown in gray, but highlighted in yellow are the LASV N elements that move upon RNA binding (α5, loop, and α6). Note that there is no structural equivalent of the α6 RNA gate in CCHFV N. (B) The amino acid sequences of 14 viral N proteins, including those from six bunyaviruses (black text) and four arenaviruses (red text), were aligned using the ClustalW online server. Virus species abbreviations (GenBank accession numbers) are as follows: DUGV, Dugbe virus (P15190.1); CCHFV, Crimean-Congo hemorrhagic fever virus (AAB48503.1); LCMV, lymphocytic choriomeningitis virus (AAX49342.1); LASV, Lassa virus (ADY11071.1); PICV, Pichinde virus (AAC32282.1); TCRV, Tacaribe virus (AAA47903.1); BUNV, Bunyamwera virus (P16495.1); LACV, La Crosse virus (AAM94389.1); RVFV, Rift Valley fever virus (ABP88854.1); HTNV, Hantaan virus (AFA36178.1); RStV; rice stripe virus (P68559.2); VSV, vesicular stomatitis virus (AAA48470.1); IAV, influenza A virus (VHIVX1); and THOV, Thogoto virus (YP_145809.1). (C) The amino acid sequences of 14 viral polymerases, including those from six bunyaviruses (black text) and four arenaviruses (red text), were aligned using the ClustalW online server. GenBank accession numbers for the L protein sequence are as follows: DUGV, Q66431; CCHFV, AAQ98866; LCMV, AB196829; LASV, CCA30313; PICV, AAL16099.1; TCRV, AAA47901.1; BUNV, P20470; LACV, Q8JPR2; RVFV, P27316; HTNV, AAG10042; RStV, ADE60705; VSV, AAA48371.1; IAV, AAA43639; and THOV, O41353. The unrooted alignments were bootstrapped using Clustal_X, and the resultant phylograms were generated using TreeView. Bootstrap confidences are labeled at each node.