The effect of forced growth of cells into 3D spheres using low attachment surfaces on the acquisition of stemness properties - PubMed (original) (raw)
The effect of forced growth of cells into 3D spheres using low attachment surfaces on the acquisition of stemness properties
Guannan Su et al. Biomaterials. 2013 Apr.
Abstract
Embryonic stem cells (ESCs) and neural progenitor cells form three-dimensional (3D) colonies or spheres in vitro, and 3D sphere is reported to help maintaining the stemness of stem cells, but the effect of 3D sphere formation on cell reprogramming remains unknown. Here we examined whether 3D sphere culture have any impact on the differentiated cells. We cultured bladder cancer cell RT4 and non-cancerous cell HEK293 on the low attachment dishes coated with soft agarose. When grown on this low attachment dish, cells spontaneously aggregated to form 3D spheres. Data showed that 3D sphere formation promoted the expression of reprogramming factors. Sphere formation of RT4 cells induced cancer stem cell characteristics including higher SP cell percentage, higher metastasis ability and higher tumorigenicity. HEK293 spheres showed upregulation of kidney progenitor cell markers and partially acquired characteristics of ESCs including upregulation of alkaline phosphatase activity, ES cell markers, three germ layer markers and tumorigenicity. The findings suggested that forced growth into 3D spheres by the low attachment surface could induce cells to acquire stemness properties.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Similar articles
- Direct conversion of fibroblasts into neural progenitor-like cells by forced growth into 3D spheres on low attachment surfaces.
Su G, Zhao Y, Wei J, Xiao Z, Chen B, Han J, Chen L, Guan J, Wang R, Dong Q, Dai J. Su G, et al. Biomaterials. 2013 Aug;34(24):5897-906. doi: 10.1016/j.biomaterials.2013.04.040. Epub 2013 May 13. Biomaterials. 2013. PMID: 23680365 - Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells.
Gao W, Wu D, Wang Y, Wang Z, Zou C, Dai Y, Ng CF, Teoh JY, Chan FL. Gao W, et al. Stem Cell Res Ther. 2018 Sep 26;9(1):243. doi: 10.1186/s13287-018-0987-x. Stem Cell Res Ther. 2018. PMID: 30257704 Free PMC article. - Anchorage-independent growth of Ewing sarcoma cells under serum-free conditions is not associated with stem-cell like phenotype and function.
Leuchte K, Altvater B, Hoffschlag S, Potratz J, Meltzer J, Clemens D, Luecke A, Hardes J, Dirksen U, Juergens H, Kailayangiri S, Rossig C. Leuchte K, et al. Oncol Rep. 2014 Aug;32(2):845-52. doi: 10.3892/or.2014.3269. Epub 2014 Jun 13. Oncol Rep. 2014. PMID: 24927333 - The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs.
Chen L, Xiao Z, Meng Y, Zhao Y, Han J, Su G, Chen B, Dai J. Chen L, et al. Biomaterials. 2012 Feb;33(5):1437-44. doi: 10.1016/j.biomaterials.2011.10.056. Epub 2011 Nov 10. Biomaterials. 2012. PMID: 22078807 - Sphere formation of ocular epithelial cells in the ciliary body is a reprogramming system for neural differentiation.
Kohno R, Ikeda Y, Yonemitsu Y, Hisatomi T, Yamaguchi M, Miyazaki M, Takeshita H, Ishibashi T, Sueishi K. Kohno R, et al. Brain Res. 2006 Jun 6;1093(1):54-70. doi: 10.1016/j.brainres.2006.03.093. Epub 2006 May 11. Brain Res. 2006. PMID: 16697356
Cited by
- Dewetting based fabrication of fibrous micro-scaffolds as potential injectable cell carriers.
Song H, Yin L, Chilian WM, Zhang Newby BM. Song H, et al. Mater Sci Eng C Mater Biol Appl. 2015 Mar;48:663-72. doi: 10.1016/j.msec.2014.12.062. Epub 2014 Dec 19. Mater Sci Eng C Mater Biol Appl. 2015. PMID: 25579969 Free PMC article. - Notch and Wnt/β-catenin signaling pathway play important roles in activating liver cancer stem cells.
Wang R, Sun Q, Wang P, Liu M, Xiong S, Luo J, Huang H, Du Q, Geller DA, Cheng B. Wang R, et al. Oncotarget. 2016 Feb 2;7(5):5754-68. doi: 10.18632/oncotarget.6805. Oncotarget. 2016. PMID: 26735577 Free PMC article. - Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering.
Rojek KO, Ćwiklińska M, Kuczak J, Guzowski J. Rojek KO, et al. Chem Rev. 2022 Nov 23;122(22):16839-16909. doi: 10.1021/acs.chemrev.1c00798. Epub 2022 Sep 15. Chem Rev. 2022. PMID: 36108106 Free PMC article. Review. - Reprogramming mediated radio-resistance of 3D-grown cancer cells.
Xue G, Ren Z, Grabham PW, Chen Y, Zhu J, Du Y, Pan D, Li X, Hu B. Xue G, et al. J Radiat Res. 2015 Jul;56(4):656-62. doi: 10.1093/jrr/rrv018. Epub 2015 Apr 16. J Radiat Res. 2015. PMID: 25883172 Free PMC article. - Enzymatically Forming Intranuclear Peptide Assemblies for Selectively Killing Human Induced Pluripotent Stem Cells.
Liu S, Zhang Q, Shy AN, Yi M, He H, Lu S, Xu B. Liu S, et al. J Am Chem Soc. 2021 Sep 29;143(38):15852-15862. doi: 10.1021/jacs.1c07923. Epub 2021 Sep 16. J Am Chem Soc. 2021. PMID: 34528792 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources