Agaricus blazei Murill as an efficient hepatoprotective and antioxidant agent against CCl4-induced liver injury in rats - PubMed (original) (raw)

Agaricus blazei Murill as an efficient hepatoprotective and antioxidant agent against CCl4-induced liver injury in rats

Abeer M Al-Dbass et al. Saudi J Biol Sci. 2012 Jul.

Abstract

Agaricus blazei Murill is one of the very popular edible medicinal mushrooms. The present study investigated the protective effect of this biologically active mushroom on the tissue peroxidative damage and abnormal antioxidant levels in carbon tetrachloride induced hepatotoxicity in male albino rats. Male albino rats of Sprague-Dawley strain weighting (120-150 g) were categorized into five groups. The first group served as the normal control, the second and the third groups were treated with Agaricus blazei Mushroom extract and carbon tetrachloride dose, respectively. Fourth group (protective group) was first treated with Agaricus blazei Mushroom extract followed by carbon tetrachloride treatment and fifth (therapeutic group) with carbon tetrachloride first followed by Agaricus blazei Mushroom treatment. The wet fruiting bodies of mushroom Agaricus blazei Murill, crushed and suspended in distilled water was administered orally to the treated groups of male albino rats. The activities of various enzymes (aspartate and alanine transaminase, lactate dehydrogenase, glutathione reductase), levels of non-enzymatic antioxidants (glutathione, vitamin C, vitamin E) and level of lipid peroxidation (malondialdehyde) were determined in the serum of all the experimental animals. Decrease in all the enzymes and non-enzymatic antioxidant, along with an increase in the lipid peroxidative index (malondialdehyde) was found in all the carbon tetrachloride treated rats as compared with normal controls. Also increase level of non-enzymatic antioxidant along with the decrease level in malondialdehyde was found in all experimental animals which were treated with Agaricus blazei Mushroom extract as compared with normal controls. The findings indicate that the extract of Agaricus blazei Murill can protect the liver against carbon tetrachloride induced oxidative damage in rats and is an efficient hepatoprotective and antioxidant agent against carbon tetrachloride induced liver injury.

Keywords: ALT, alanine transaminase; AST, aspartate transaminase; AbM, Agaricus blazei Murill; Agaricus blazei Murill; Antioxidant; CCl4, carbon tetrachloride; Carbon tetrachloride; GR, glutathione reductase; GSH, glutathione; Hepatoprotective; LDH, lactate dehydrogenase; Liver; MDA, malondialdehyde; vit. C, vitamin C; vit. E, vitamin E.

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Figures

Figure 1

Figure 1

Percentage change of LDH, ALT, AST and GR in CCl4-treated, CCl4-treated + then Mushroom dose, Protected by Mushroom + then CCl4-treated and Protected by Mushroom groups compared to control.

Figure 2

Figure 2

Percentage change of Vit. C, Vit. E, MDA and GSH in CCl4-treated, CCl4-treated + then Mushroom dose, Protected by Mushroom + then CCl4-treated and Protected by Mushroom groups compared to control.

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