Neuroinflammation and α-synuclein accumulation in response to glucocerebrosidase deficiency are accompanied by synaptic dysfunction - PubMed (original) (raw)

. 2014 Feb;111(2):152-62.

doi: 10.1016/j.ymgme.2013.12.003. Epub 2013 Dec 11.

Sally K-K Mak 2, Novie Ko 2, Juliane Karlgren 3, Schahram Akbarian 4, Vivian P Chou 2, Yin Guo 4, Arlene Lim 5, Steven Samuelsson 6, Mary L LaMarca 7, Jacqueline Vazquez-DeRose 6, Amy B Manning-Boğ 8

Affiliations

Neuroinflammation and α-synuclein accumulation in response to glucocerebrosidase deficiency are accompanied by synaptic dysfunction

Edward I Ginns et al. Mol Genet Metab. 2014 Feb.

Abstract

Clinical, epidemiological and experimental studies confirm a connection between the common degenerative movement disorder Parkinson's disease (PD) that affects over 1 million individuals, and Gaucher disease, the most prevalent lysosomal storage disorder. Recently, human imaging studies have implicated impaired striatal dopaminergic neurotransmission in early PD pathogenesis in the context of Gaucher disease mutations, but the underlying mechanisms have yet to be characterized. In this report we describe and characterize two novel long-lived transgenic mouse models of Gba deficiency, along with a subchronic conduritol-ß-epoxide (CBE) exposure paradigm. All three murine models revealed striking glial activation within nigrostriatal pathways, accompanied by abnormal α-synuclein accumulation. Importantly, the CBE-induced, pharmacological Gaucher mouse model replicated this change in dopamine neurotransmission, revealing a markedly reduced evoked striatal dopamine release (approximately 2-fold) that indicates synaptic dysfunction. Other changes in synaptic plasticity markers, including microRNA profile and a 24.9% reduction in post-synaptic density size, were concomitant with diminished evoked dopamine release following CBE exposure. These studies afford new insights into the mechanisms underlying the Parkinson's-Gaucher disease connection, and into the physiological impact of related abnormal α-synuclein accumulation and neuroinflammation on nigrostriatal dopaminergic neurotransmission.

Keywords: Gaucher disease; Glucocerebrosidase; Neuroinflammation; Parkinson's disease; Synaptic dysfunction; α-Synuclein.

Copyright © 2013 Elsevier Inc. All rights reserved.

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