Proteomic analysis of embryogenesis and the acquisition of seed dormancy in Norway maple (Acer platanoides L.) - PubMed (original) (raw)
Proteomic analysis of embryogenesis and the acquisition of seed dormancy in Norway maple (Acer platanoides L.)
Aleksandra Maria Staszak et al. Int J Mol Sci. 2014.
Abstract
The proteome of zygotic embryos of Acer platanoides L. was analyzed via high-resolution 2D-SDS-PAGE and MS/MS in order to: (1) identify significant physiological processes associated with embryo development; and (2) identify changes in the proteome of the embryo associated with the acquisition of seed dormancy. Seventeen spots were identified as associated with morphogenesis at 10 to 13 weeks after flowering (WAF). Thirty-three spots were associated with maturation of the embryo at 14 to 22 WAF. The greatest changes in protein abundance occurred at 22 WAF, when seeds become fully mature. Overall, the stage of morphogenesis was characterized by changes in the abundance of proteins (tubulins and actin) associated with the growth and development of the embryo. Enzymes related to energy supply were especially elevated, most likely due to the energy demand associated with rapid growth and cell division. The stage of maturation is crucial to the establishment of seed dormancy and is associated with a higher abundance of proteins involved in genetic information processing, energy and carbon metabolism and cellular and antioxidant processes. Results indicated that a glycine-rich RNA-binding protein and proteasome proteins may be directly involved in dormancy acquisition control, and future studies are warranted to verify this association.
Figures
Figure 1
Embryogenesis of A. platanoides L. from 10 through 14 weeks after flowering (WAF). Bar = 5 mm.
Figure 2
Protein content (columns) and fresh weight (line) of embryos of A. platanoides L. during morphogenesis: (A) 10 through 13 weeks after flowering (WAF) and maturation; (B) 14–22 WAF. Error bars represent SD (n = 3).
Figure 3
Representative images showing spot identification and localization of proteins extracted from embryos of A. platanoides during morphogenesis (A) and maturation (B). Proteins that varied in abundance during the period of embryo morphogenesis (10–13 weeks after flowering, WAF) and embryo maturation (14–22 WAF) are indicated by the yellow boxes and blue indicators. Complete information on these proteins is provided in Table 1, Table 2 and Table S1.
Similar articles
- Proteome analysis of Norway maple (Acer platanoides L.) seeds dormancy breaking and germination: influence of abscisic and gibberellic acids.
Pawłowski TA. Pawłowski TA. BMC Plant Biol. 2009 May 4;9:48. doi: 10.1186/1471-2229-9-48. BMC Plant Biol. 2009. PMID: 19413897 Free PMC article. - DNA synthesis pattern, proteome, and ABA and GA signalling in developing seeds of Norway maple (Acer platanoides).
Staszak AM, Rewers M, Sliwinska E, Klupczynska EA, Pawlowski TA. Staszak AM, et al. Funct Plant Biol. 2019 Jan;46(2):152-164. doi: 10.1071/FP18074. Funct Plant Biol. 2019. PMID: 32172757 - Proteomic approach to analyze dormancy breaking of tree seeds.
Pawłowski TA. Pawłowski TA. Plant Mol Biol. 2010 May;73(1-2):15-25. doi: 10.1007/s11103-010-9623-6. Epub 2010 Mar 21. Plant Mol Biol. 2010. PMID: 20306286 - Proteomics of seed development, desiccation tolerance, germination and vigor.
Wang WQ, Liu SJ, Song SQ, Møller IM. Wang WQ, et al. Plant Physiol Biochem. 2015 Jan;86:1-15. doi: 10.1016/j.plaphy.2014.11.003. Epub 2014 Nov 4. Plant Physiol Biochem. 2015. PMID: 25461695 Review. - Emerging Roles of RNA-Binding Proteins in Seed Development and Performance.
Lou L, Ding L, Wang T, Xiang Y. Lou L, et al. Int J Mol Sci. 2020 Sep 17;21(18):6822. doi: 10.3390/ijms21186822. Int J Mol Sci. 2020. PMID: 32957608 Free PMC article. Review.
Cited by
- Current Proteomic and Metabolomic Knowledge of Zygotic and Somatic Embryogenesis in Plants.
Juarez-Escobar J, Bojórquez-Velázquez E, Elizalde-Contreras JM, Guerrero-Analco JA, Loyola-Vargas VM, Mata-Rosas M, Ruiz-May E. Juarez-Escobar J, et al. Int J Mol Sci. 2021 Oct 30;22(21):11807. doi: 10.3390/ijms222111807. Int J Mol Sci. 2021. PMID: 34769239 Free PMC article. Review. - Roles of Plant Glycine-Rich RNA-Binding Proteins in Development and Stress Responses.
Ma L, Cheng K, Li J, Deng Z, Zhang C, Zhu H. Ma L, et al. Int J Mol Sci. 2021 May 29;22(11):5849. doi: 10.3390/ijms22115849. Int J Mol Sci. 2021. PMID: 34072567 Free PMC article. Review. - Nicotinamide adenine dinucleotides are associated with distinct redox control of germination in Acer seeds with contrasting physiology.
Alipour S, Bilska K, Stolarska E, Wojciechowska N, Kalemba EM. Alipour S, et al. PLoS One. 2021 Jan 27;16(1):e0245635. doi: 10.1371/journal.pone.0245635. eCollection 2021. PLoS One. 2021. PMID: 33503034 Free PMC article. - Integration of MsrB1 and MsrB2 in the Redox Network during the Development of Orthodox and Recalcitrant Acer Seeds.
Stolarska E, Bilska K, Wojciechowska N, Bagniewska-Zadworna A, Rey P, Kalemba EM. Stolarska E, et al. Antioxidants (Basel). 2020 Dec 9;9(12):1250. doi: 10.3390/antiox9121250. Antioxidants (Basel). 2020. PMID: 33316974 Free PMC article. - Involvement of the MetO/Msr System in Two Acer Species That Display Contrasting Characteristics during Germination.
Wojciechowska N, Alipour S, Stolarska E, Bilska K, Rey P, Kalemba EM. Wojciechowska N, et al. Int J Mol Sci. 2020 Dec 2;21(23):9197. doi: 10.3390/ijms21239197. Int J Mol Sci. 2020. PMID: 33276642 Free PMC article.
References
- Bewley J.D., Bradford K., Hilhorst H., Nonogaki H. Seeds—Physiology of Development, Germination and Dormancy. 3rd ed. Springer; Berlin, Germany: 2013.
- Baskin J., Baskin C. A classification system for seed dormancy. Seed Sci. Res. 2004;14:1–16.
- Hilhorst H.W.M. A critical update on seed dormancy. I. Primary dormancy. Seed Sci. Res. 1995;5:61–73.
- Black M., Bewley J.D., Halmer P. The Encyclopedia of Seeds. Science, Technology and Uses. CABI; Wallingford, UK: 2008.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources