Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus - PubMed (original) (raw)

Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus

Byung Soon Hwang et al. Mycobiology. 2014 Jun.

Abstract

During a search for neuraminidase inhibitors derived from medicinal fungi, we found that the fermentation broth of Phellinus linteus exhibited potent neuraminidase inhibitory activity. Through bioassay-guided fractionation, two active compounds were purified from the ethyl acetate-soluble portion of the fermentation broth of P. linteus. These structures were identified as inotilone (1) and 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2) by spectroscopic methods. Compounds 1 and 2 inhibited H1N1 neuraminidase activity with IC50 values of 29.1 and 125.6 µM, respectively, in a dose-dependent manner. They also exhibited an antiviral effect in a viral cytopathic effect reduction assay using MDCK cells. These results suggest that compounds 1 and 2 from the culture broth of P. linteus would be good candidates for the prevention and therapeutic strategies towards viral infections.

Keywords: 4-(3,4-Dihydroxyphenyl)-3-buten-2-one; Anti-influenza agent; Inotilone; Neuraminidase inhibitor; Phellinus linteus.

PubMed Disclaimer

Figures

Fig. 1

Structures of compounds 1 (inotilone) and 2 (4-(3,4-dihydroxyphenyl)-3-buten-2-one).

Fig. 2

Effects of compounds 1 and 2 on influenza A/WS/33 virus-induced cytopathic effect.

Cited by

Discovery and characterization of a novel peptide inhibitor against influenza neuraminidase.
Chen J, Feng S, Xu Y, Huang X, Zhang J, Chen J, An X, Zhang Y, Ning X. Chen J, et al. RSC Med Chem. 2020 Jan 22;11(1):148-154. doi: 10.1039/c9md00473d. eCollection 2020 Jan 1. RSC Med Chem. 2020. PMID: 33479615 Free PMC article.
Chemical Constituents of the Culture Broth of Phellinus linteus and Their Antioxidant Activity.
Lee MS, Hwang BS, Lee IK, Seo GS, Yun BS. Lee MS, et al. Mycobiology. 2015 Mar;43(1):43-8. doi: 10.5941/MYCO.2015.43.1.43. Epub 2015 Mar 31. Mycobiology. 2015. PMID: 25892914 Free PMC article.
A concise review of mushrooms antiviral and immunomodulatory properties that may combat against COVID-19.
Arunachalam K, Sasidharan SP, Yang X. Arunachalam K, et al. Food Chem Adv. 2022 Oct;1:100023. doi: 10.1016/j.focha.2022.100023. Epub 2022 Mar 2. Food Chem Adv. 2022. PMID: 36686330 Free PMC article. Review.
A Review: The Bioactivities and Pharmacological Applications of Phellinus linteus.
Chen W, Tan H, Liu Q, Zheng X, Zhang H, Liu Y, Xu L. Chen W, et al. Molecules. 2019 May 16;24(10):1888. doi: 10.3390/molecules24101888. Molecules. 2019. PMID: 31100959 Free PMC article. Review.
Protective Effect of Osmundacetone against Neurological Cell Death Caused by Oxidative Glutamate Toxicity.
Trinh TA, Seo YH, Choi S, Lee J, Kang KS. Trinh TA, et al. Biomolecules. 2021 Feb 22;11(2):328. doi: 10.3390/biom11020328. Biomolecules. 2021. PMID: 33671577 Free PMC article.

References

1. Palese P, Shaw ML. Orthomyxoviridae: the viruses and their replication. In: Knipe DM, Howley PM, editors. Fields virology. 5th ed. Philadelphia: Lippincott Williams & Wilkins; 2007. pp. 1647–1689.
1. Zhang J, Yu K, Zhu W, Jiang H. Neuraminidase pharmacophore model derived from diverse classes of inhibitors. Bioorg Med Chem Lett. 2006;16:3009–3014. - PubMed
1. Moscona A. Neuraminidase inhibitors for influenza. N Engl J Med. 2005;353:1363–1373. - PubMed
1. Nicholson KG, Wood JM, Zambon M. Influenza. Lancet. 2003;362:1733–1745. - PMC - PubMed
1. Deng K, Zhang Y, Xie L, Peng W, Gan B, Ren Z. Simultaneous determination of five fatty acids in Phellinus sp. by high-performance liquid chromatography with photodiode-array detection. J Med Plant Res. 2011;5:2816–2821.

LinkOut - more resources

Full Text Sources
Other Literature Sources
- The Lens - Patent Citations Database
- scite Smart Citations

Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus - PubMed (original) (raw)