Thioacetamide-induced liver injury: protective role of genistein - PubMed (original) (raw)
. 2014 Nov;92(11):965-73.
doi: 10.1139/cjpp-2014-0192. Epub 2014 Sep 9.
Affiliations
- PMID: 25358106
- DOI: 10.1139/cjpp-2014-0192
Thioacetamide-induced liver injury: protective role of genistein
Dalia O Saleh et al. Can J Physiol Pharmacol. 2014 Nov.
Abstract
This study aimed to investigate the possible protective effects of genistein (GEN), a phytoestrogen, on the liver injury induced in rats by thioacetamide (TTA; 200.0 mg·(kg body mass)(-1); administered 3 times a week by intraperitoneal injection). GEN (0.5, 1.0, or 2.0 mg·(kg body mass)(-1); by subcutaneous injection) was concurrently administered on a daily basis for 8 weeks, and its effects were evaluated 24 h after the administration of the last dose. The results from this study revealed that TTA-induced liver injury was associated with massive changes in the serum levels of liver biomarkers, oxidative stress markers, and liver inflammatory cytokines. Treatment of TAA-induced liver injury in rats with GEN decreased the elevated serum levels of aspartate aminotransferase, alanine aminotransferase, and total and direct bilirubin, and increased the serum level of albumin. GEN also restored the liver levels of malondialdehyde and reduced glutathione, as well as tumor necrosis factor-alpha, interleukin-6, and their modulator nuclear factor kappa-light-chain-enhancer of activated B cells. From our results, it can be concluded that GEN attenuates the liver injury-induced in rats with TAA, and this hepatoprotective role is attributed to its anti-inflammatory and antioxidant properties.
Keywords: anti-oxydant; antioxidant; biomarqueur hépatique; cytokines inflammatoires; hepatoprotective; hépato-protecteur; inflammatory cytokines; liver biomarker; phytoestrogen; phytoestrogène.
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