Peculiarities of induction and persistence of hidden chromosome instability in peripheral blood lymphocytes of persons occupationally exposed to ionizing radiation - PubMed (original) (raw)

[Article in English, Ukrainian]

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PMID: 25536569

Peculiarities of induction and persistence of hidden chromosome instability in peripheral blood lymphocytes of persons occupationally exposed to ionizing radiation

[Article in English, Ukrainian]

M A Pilinska et al. Probl Radiac Med Radiobiol. 2014 Sep.

Abstract

Objective - to investigate the induction of hidden chromosome instability in persons occupationally exposed to ionizing radiation and its persistence in vitro in successive mitoses. Materials and methods. Using two tests ("G2-bleomycin sensitivity assay" and two-term cultivation of human peripheral blood lymphocytes) voluntary cytogenetic examination of 15 individuals participated in the conversion of the "Shelter" ("Chornobyl NPP") into ecologically safe system had been carried out. Total 24 034 metaphase had been analyzed, of which 12 243 - without additional mutagenic exposure, 11 791 - exposed to bleomycin in vitro at concentration of 0.05 μg/ml. Results. The magnitude and dynamics of background as well as bleomycin-induced cytogenetic effects in both terms of lymphocytes' cultivation in occupational group differed significantly from the group of comparison towards increasing of chromosome instability indices with significant interindividual fluctuations. Conclusion. Interindividual differences in persistence of radiation-induced hidden chromosome instability in successive generations of human somatic cells had been found.

Meta doslidzhennja. Doslidyty indukciju pryhovanoi' hromosomnoi' nestabil'nosti u osib, jaki profesijno kontaktuvaly z ionizujuchoju radiacijeju, ta i'i' persystenciju in vitro v poslidovnyh mitozah. Materialy i metody. Z vykorystannjam testiv „G2-bleomycin sensitivity assay” ta dvoterminovogo kul'tyvuvannja limfocytiv peryferychnoi' krovi provedeno dobrovil'ne cytogenetychne obstezhennja 15 osib, jaki braly uchast' u robotah z peretvorennja ob’jektu „Ukryttja” DSP „Chornobyl's'ka AES” na ekologichno bezpechnu systemu. Vs'ogo proanalizovano 24 034 metafazy, z jakyh 12 243 – bez mutagennogo navantazhennja; 11 791 – pry dii' bleomicynu in vitro v koncentracii' 0,05 mkg/ml. Rezul'taty. Za velychynoju ta dynamikoju fonovogo ta indukovanogo bleomicynom cytogenetychnyh efektiv pry riznyh strokah kul'tyvuvannja limfocytiv profesijna grupa virogidno vidriznjalys' vid grupy porivnjannja v bik zrostannja pokaznykiv hromosomnoi' nestabil'nosti z suttjevymy mizhindyvidual'nymy kolyvannjamy. Vysnovok. Vstanovleno mizhindyvidual'ni vidminnosti persystencii' radiacijno-indukovanoi' pryhovanoi' hromosomnoi' nestabil'nosti v poslidovnyh generacijah somatychnyh klityn ljudyny.

Keywords: bleomycin; hidden chromosome instability; ionizing radiation; persisting of cytogenetic effect in successive mitosis; testing mutagenic exposure.

M. A. Pilinska, S. S. Dybsky, O. B. Dybska, L. I. Shvayko, V. O. Sushko.

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Peculiarities of induction and persistence of hidden chromosome instability in peripheral blood lymphocytes of persons occupationally exposed to ionizing radiation - PubMed (original) (raw)