The Effectiveness of Crataegus orientalis M Bieber. (Hawthorn) Extract Administration in Preventing Alveolar Bone Loss in Rats with Experimental Periodontitis - PubMed (original) (raw)

The Effectiveness of Crataegus orientalis M Bieber. (Hawthorn) Extract Administration in Preventing Alveolar Bone Loss in Rats with Experimental Periodontitis

Mükerrem Hatipoğlu et al. PLoS One. 2015.

Abstract

The purpose of this animal study was to evaluate the effects of hawthorn (Crataeus orientalis M Bieber.) extract on serum oxidative status and alveolar bone loss in experimental periodontitis. Twenty-seven Wistar rats were assigned to one of the following groups: non- ligated+placebo (saline) (NL, n = 9), ligature only+placebo (saline) (LO, n = 9), and ligature and treated with hawthorn extract in saline (H, n = 9) (100 mg/kg orogastrically, once a day for 11 days). Periodontitis was induced by submerging a 4/0 silk ligature in the sulcus of the mandibular right first molars of rats, and the animals were sacrificed after 11 days. Micro-CT examinations were performed for linear and volumetric parameter assessment of alveolar bone. Periodontal tissues were histopathologically examined to assess the differences among the study groups. Levels of serum total antioxidant status (TAS)/total oxidant status (TOS), and oxidative stress index (OSI) were also analyzed. Alveolar bone loss was significantly reduced by hawthorn administration compared to LO group (p<0.05). The number of inflammatory cells and osteoclasts in the LO group was significantly higher than that of the NL and H groups (p< 0.05). The number of osteoblasts in the LO and H groups was significantly higher than that of the NL group (p<0.05). TOS and OSI levels were significantly reduced in H group compared to LO group (P <0.05) and TAS levels were similar in H and NL group (p< 0.05). Hawthorn extract showed inhibitory effect on periodontal inflammation and alveolar bone loss by regulating TAS, TOS and OSI levels in periodontal disease in rats when administered systemically.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1

Fig 1. A- Linear measurements are taken of ABL in the interdental space from the CEJ to ABC.

(Green line: CEJ, red line: ABC, yellow line: Distance (mm)). B- Axially, a rectangular-shaped region of interest that is 0.33 mm far from each direction of tooth crown. Vertically, a rectangular-shaped region of interest include coronal halves of the slices through mesial root apex to the CEJ. C- Volumetric measurement with 3-D generated ROI.

Fig 2

Fig 2. The 3D representative images of each group.

A- NL group. B- LO group. C- H group.

Fig 3

Fig 3. A- Mean ABL in study groups (mm).

B- Alveolar bone volume in study groups (mm3). * Significant difference compared to NL group. † Significant difference compared to LO.

Fig 4

Fig 4. The histological representive images of each experimental group.

A- The histological images of NL group showing normal periodontium (red arrows: erythrocytes). B- LO group showing intense inflammatory cells (green arrow: lymphocyte, black arrow: neutrophils) and dilated blood vessels C- The histological images of H group showing moderate inflammatory cell infiltrate in periodontal ligament (Yellow arrows show inflammatory cells). (ab: alveolar bone, pdl: periodontal ligament).

Fig 5

Fig 5. A- The number of inflammatory cells (/mm2) in the study groups.

B- The number of osteoclasts (/mm2) in the study groups. C- The number of osteoblasts (/mm2) in the study groups. D- TAS in the study groups. E- TOS in the study groups. F- OSI levels in the study groups. * Significant difference compared to NL group. † Significant difference compared to LO.

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