Lysyl oxidase is associated with the epithelial-mesenchymal transition of gastric cancer cells in hypoxia - PubMed (original) (raw)

. 2016 Apr;19(2):431-442.

doi: 10.1007/s10120-015-0510-3. Epub 2015 Jun 23.

Affiliations

• PMID: 26100130
• DOI: 10.1007/s10120-015-0510-3

Lysyl oxidase is associated with the epithelial-mesenchymal transition of gastric cancer cells in hypoxia

Hiroaki Kasashima et al. Gastric Cancer. 2016 Apr.

Abstract

Purpose: It has been reported that lysyl oxidase (LOX) is a hypoxia-responsive factor and is associated with the malignant progression of carcinoma. The aim of this study was to clarify the relationship between the epithelial-mesenchymal transition (EMT) and LOX in gastric cancer cells under hypoxia.

Methods: Two gastric cancer cell lines, OCUM-2MD3 and OCUM-12, were used in an in vitro study. The effect of LOX small interfering RNA (siRNA) on the EMT and motility of gastric cancer cells under hypoxic condition was analyzed by reverse transcription PCR, Western blot, a wound-healing assay, and an invasion assay. Correlations between LOX expression and the clinicopathological features of 544 patients with gastric carcinoma were examined immunohistochemically.

Results: Hypoxic conditions increased the number of polygonal or spindle-shaped cells resulting from EMT in gastric cancer cells. The EMT of cancer cells induced by hypoxia was inhibited by treatment with LOX siRNA. The number of migrating and invading gastric cancer cells in hypoxia was significantly decreased by LOX knockdown. LOX siRNA significantly increased the E-cadherin level and decreased the vimentin level of gastric cancer cells. LOX expression was significantly associated with invasion depth, tumor differentiation, lymph node metastasis, lymphatic invasion, venous invasion, and peritoneal metastasis. Multivariable analysis revealed that LOX was an independent parameter for overall survival.

Conclusion: LOX affects the EMT of gastric cancer cells in hypoxic conditions. LOX expression is a useful prognostic factor for patients with gastric cancer.

Keywords: Epithelial–mesenchymal transition; Gastric cancer; Lysyl oxidase.

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References

1. 1. Biol Open. 2014 Feb 15;3(2):129-37 - PubMed
2. 1. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17460-5 - PubMed
3. 1. Cancer Res. 2002 Nov 15;62(22):6645-50 - PubMed
4. 1. J Cell Biochem. 2003 Mar 1;88(4):660-72 - PubMed
5. 1. Nature. 2006 Apr 27;440(7088):1222-6 - PubMed

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