A Microbiopsy Method for Immunohistological and Morphological Analysis: A Pilot Study - PubMed (original) (raw)
A Microbiopsy Method for Immunohistological and Morphological Analysis: A Pilot Study
Jeremy R Townsend et al. Med Sci Sports Exerc. 2016 Feb.
Abstract
Introduction: The fine aspiration microbiopsy is a relatively new biopsy technique, which allows muscle physiologists to sample skeletal muscle less invasively. However, the small sample size obtained is often deemed insufficient for certain analyses. The aim of the current study was to develop procedures for muscle fiber morphology and immunohistological analysis from a microbiopsy technique.
Methods: Microbiopsies of the vastus lateralis were taken with a 14-gauge microbiopsy needle from four healthy men on two separate occasions. The tissue was oriented in a cryomold, embedded in Tissue-Tek® then frozen in liquid nitrogen cooled isopentane. The muscle sections were stained with hematoxylin and eosin, laminin, MHCI, MHCIIa, and Pax7 for fiber number, mean fiber area, muscle fiber typing, and satellite cell observation.
Results: The mean ± SD (range) microbiopsy sample weight was 18.3 ± 2.9 mg (14-22 mg). The mean fiber number within the microbiopsy specimens was 150.4 ± 120.6 (64-366). All viable fibers were measured in each sample, and the mean fiber area was 4385.1 ± 1265.8 μm2 (977.0-10,132.93 μm2). There was no significant time difference (P = 0.69) in mean fiber area.
Discussion: Results suggest the potential use of a "minimally invasive" muscle biopsy technique for immunohistological and morphological analysis. This could provide clinicians and investigators additional data in future research. Further investigations are needed to determine the usefulness and potential limiting factors of this technique.
Similar articles
- Microbiopsy Sampling for Examining Age-Related Differences in Skeletal Muscle Fiber Morphology and Composition.
Hester GM, VanDusseldorp TA, Ha PL, Kiani K, Olmos AA, Jabbari M, Kalladanthyil S, An S, Bailly AR, Dalton BE, Bryantsev AL. Hester GM, et al. Front Physiol. 2022 Jan 10;12:756626. doi: 10.3389/fphys.2021.756626. eCollection 2021. Front Physiol. 2022. PMID: 35082686 Free PMC article. - Skeletal muscle microbiopsy: a validation study of a minimally invasive technique.
Hayot M, Michaud A, Koechlin C, Caron MA, Leblanc P, Préfaut C, Maltais F. Hayot M, et al. Eur Respir J. 2005 Mar;25(3):431-40. doi: 10.1183/09031936.05.00053404. Eur Respir J. 2005. PMID: 15738285 Clinical Trial. - Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal mitochondrial respiration rate.
Isner-Horobeti ME, Charton A, Daussin F, Geny B, Dufour SP, Richard R. Isner-Horobeti ME, et al. Eur J Appl Physiol. 2014 May;114(5):885-9. doi: 10.1007/s00421-014-2819-8. Epub 2014 Jan 22. Eur J Appl Physiol. 2014. PMID: 24449221 - Fine-needle percutaneous muscle microbiopsy technique as a feasible tool to address histological analysis in young children with cerebral palsy and age-matched typically developing children.
Deschrevel J, Maes K, Andries A, Beukelaer N, Corvelyn M, Costamagna D, Campenhout AV, Wachter E, Desloovere K, Agten A, Vandenabeele F, Nijs S, Gayan-Ramirez G. Deschrevel J, et al. PLoS One. 2023 Nov 22;18(11):e0294395. doi: 10.1371/journal.pone.0294395. eCollection 2023. PLoS One. 2023. PMID: 37992082 Free PMC article. - The skeletal muscle microbiopsy method in exercise and sports science research: A narrative and methodological review.
Newmire DE, Willoughby DS. Newmire DE, et al. Scand J Med Sci Sports. 2022 Nov;32(11):1550-1568. doi: 10.1111/sms.14215. Epub 2022 Aug 24. Scand J Med Sci Sports. 2022. PMID: 35904526 Review.
Cited by
- Microbiopsy Sampling for Examining Age-Related Differences in Skeletal Muscle Fiber Morphology and Composition.
Hester GM, VanDusseldorp TA, Ha PL, Kiani K, Olmos AA, Jabbari M, Kalladanthyil S, An S, Bailly AR, Dalton BE, Bryantsev AL. Hester GM, et al. Front Physiol. 2022 Jan 10;12:756626. doi: 10.3389/fphys.2021.756626. eCollection 2021. Front Physiol. 2022. PMID: 35082686 Free PMC article. - The Effect of ProHydrolase® on the Amino Acid and Intramuscular Anabolic Signaling Response to Resistance Exercise in Trained Males.
Townsend JR, Morimune JE, Jones MD, Beuning CN, Haase AA, Boot CM, Heffington SH, Littlefield LA, Henry RN, Marshall AC, VanDusseldorp TA, Feito Y, Mangine GT. Townsend JR, et al. Sports (Basel). 2020 Jan 22;8(2):13. doi: 10.3390/sports8020013. Sports (Basel). 2020. PMID: 31978998 Free PMC article. - Influence of Skeletal Muscle Carnosine Content on Fatigue during Repeated Resistance Exercise in Recreationally Active Women.
Varanoske AN, Hoffman JR, Church DD, Wang R, Baker KM, Dodd SJ, Coker NA, Oliveira LP, Dawson VL, Fukuda DH, Stout JR. Varanoske AN, et al. Nutrients. 2017 Sep 7;9(9):988. doi: 10.3390/nu9090988. Nutrients. 2017. PMID: 28880219 Free PMC article. - Sarcopenia versus cancer cachexia: the muscle wasting continuum in healthy and diseased aging.
Moreira-Pais A, Ferreira R, Oliveira PA, Duarte JA. Moreira-Pais A, et al. Biogerontology. 2021 Oct;22(5):459-477. doi: 10.1007/s10522-021-09932-z. Epub 2021 Jul 29. Biogerontology. 2021. PMID: 34324116 Review. - Polyphenol supplementation alters intramuscular apoptotic signaling following acute resistance exercise.
Townsend JR, Stout JR, Jajtner AR, Church DD, Beyer KS, Riffe JJ, Muddle TWD, Herrlinger KL, Fukuda DH, Hoffman JR. Townsend JR, et al. Physiol Rep. 2018 Jan;6(2):e13552. doi: 10.14814/phy2.13552. Physiol Rep. 2018. PMID: 29380956 Free PMC article. Clinical Trial.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous