Antibiotic susceptibility profile of bacilli isolated from the skin of healthy humans - PubMed (original) (raw)

Antibiotic susceptibility profile of bacilli isolated from the skin of healthy humans

Prashant Tarale et al. Braz J Microbiol. 2015 Oct-Dec.

Abstract

In the present work, twelve bacilli were isolated from four different regions of human skin from Bela population of Nagpur district, India. The isolated bacilli were identified by their morphological, cultural and biochemical characteristics. Seven isolates were Gram negative rods, out of which five were belong to genus Pseudomonas. Three among the five Gram positive isolates were identified as Dermabactor and the remaining two Bacillus. Their antimicrobial susceptibility profile was determined by Kirby-Bauer disc diffusion method. The isolates showed resistance to several currently used broad-spectrum antibiotics. The Dermabactor genus was resistant to vancomycin, although it was earlier reported to be susceptible. Imipenem was found to be the most effective antibiotic for Pseudomonas while nalidixic acid, ampicillin and tetracycline were ineffective. Isolates of Bacillus displayed resistance to the extended spectrum antibiotics cephalosporin and ceftazidime. Imipenem, carbenicillin and ticarcillin were found to be the most effective antibiotics as all the investigated isolates were susceptible to them. Antibiotic resistance may be due to the overuse or misuse of antibiotics during the treatment, or following constant exposure to antibiotic-containing cosmetic formulations.

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Figures

Figure 1

Figure 1. Representative result for antibiotic susceptibility profile. Zone of inhibition obtained on Mueller Hinton (MH) agar for (A) 10IE isolate and (B) 7IE2 isolate.

Figure 2

Figure 2. Representative result for lipid hydrolysis on tributyrin agar.

Figure 3

Figure 3. Agarose gel showing amplification of 16S rRNA genes from different isolates. Lane 1- 9IE, 2- 4IE, 3- 15IE, 4- 7IE2, 5- 7J, 6- 10IE, 7- 10J, 8- 14J, 9- 14FH, 10- 14C, 11- 12JY, 12- 13C, 13- 5CT.

Figure 4

Figure 4. Agarose gel amplification of Lipase A gene from different isolates. Lane 54- 9IE, 55- 4IE, 56- 15IE, 57- 7IE2, 58- 7J, 59- 10IE, 60- 10J, 61- 14J, 62- 14FH, 63- 14C, 64- 12JY, 65- 13C, 66- 5CT.

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