Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes - PubMed (original) (raw)
Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes
Kyung-Hee Lee et al. Prev Nutr Food Sci. 2015 Dec.
Abstract
The anti-obesity effects of starter (Leuconostoc mesenteroides+Lactobacillus plantarum) fermented kimchi on 3T3-L1 adipocyte were studied using naturally fermented kimchi (NK), a functional kimchi (FK, NK supplemented with green tea), and FK supplemented with added starters (FKS). Oil red O staining and cellular levels of triglyceride (TG) and glycerol were used to evaluate the in vitro anti-obesity effects of these kimchis in 3T3-L1 cells. The expressions of adipogenesis/lipogenesis-related genes of peroxisome proliferator-active receptor (PPAR)-γ, CCAAT/enhance-binding protein (C/EBP)-α, and fatty acid synthase (FAS) were determined by RT-PCR. Kimchis, especially FKS, markedly decreased TG levels and increased levels of intracellular glycerol and lipid lipolysis. In addition, FKS also reduced the mRNA levels of PPAR-γ, C/EBP-α, and FAS, which are related to adipogenesis/lipogenesis in 3T3-L1 cells. These results suggest the anti-obesity effects of FKS were to due to enhanced lipolysis and reduced adipogenesis/lipogenesis in 3T3-L1 adipocytes.
Keywords: 3T3-L1 cells; antiobesity; kimchi; starters.
Figures
Fig. 1
Effect of kimchi samples on Oil red O staining in 3T3-L1 adipocytes. Con, control; NK, naturally fermented kimchi; FK, functional kimchi; FKS, functional kimchi with starters.
Fig. 2
Effect of kimchi samples on the mRNA expressions of PPAR-γ, C/EBPα, and FAS in 3T3-L1 adipocytes. The PCR products were quantified and normalized GAPDH (internal control). Band intensities were measured using a densitometer and expressed as folds versus treatment naïve controls. Fold increase=gene expression/GAPDH×control value (control fold increase=1). NK, naturally fermented kimchi; FK, functional kimchi; FKS, functional kimchi produced using prebiotic starters.
Similar articles
- Anti-Obesity Effect of Kimchi with Starter Cultures in 3T3-L1 Cells.
Hyun IK, Hong SW, Ma MJ, Chang JY, Lee S, Yun YR. Hyun IK, et al. J Microbiol Biotechnol. 2024 Jan 28;34(1):123-131. doi: 10.4014/jmb.2307.07005. Epub 2023 Oct 11. J Microbiol Biotechnol. 2024. PMID: 37830224 Free PMC article. - Korean Chungtaejeon tea extract attenuates body weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes.
Sharma BR, Kim DW, Rhyu DY. Sharma BR, et al. J Integr Med. 2017 Jan;15(1):56-63. doi: 10.1016/S2095-4964(17)60321-2. J Integr Med. 2017. PMID: 28088260 - Tetrandrine has anti-adipogenic effect on 3T3-L1 preadipocytes through the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.
Jang BC. Jang BC. Biochem Biophys Res Commun. 2016 Aug 5;476(4):481-486. doi: 10.1016/j.bbrc.2016.05.150. Epub 2016 May 28. Biochem Biophys Res Commun. 2016. PMID: 27246736 - Cissus quadrangularis extract (CQR-300) inhibits lipid accumulation by downregulating adipogenesis and lipogenesis in 3T3-L1 cells.
Lee HJ, Le B, Lee DR, Choi BK, Yang SH. Lee HJ, et al. Toxicol Rep. 2018 Mar 30;5:608-614. doi: 10.1016/j.toxrep.2018.02.008. eCollection 2018. Toxicol Rep. 2018. PMID: 29854631 Free PMC article. - Cellular models for the evaluation of the antiobesity effect of selected phytochemicals from food and herbs.
Tung YC, Hsieh PH, Pan MH, Ho CT. Tung YC, et al. J Food Drug Anal. 2017 Jan;25(1):100-110. doi: 10.1016/j.jfda.2016.10.018. Epub 2016 Dec 7. J Food Drug Anal. 2017. PMID: 28911527 Free PMC article. Review.
Cited by
- Exploring the anti-obesity effects of kimchi through enhanced thermogenesis in differentiated T37i brown adipocytes.
Yun YR, Lee JE, Lee S, Hong SW. Yun YR, et al. Food Nutr Res. 2024 Aug 29;68. doi: 10.29219/fnr.v68.10738. eCollection 2024. Food Nutr Res. 2024. PMID: 39239457 Free PMC article. - Insights into the Anti-Adipogenic and Anti-Inflammatory Potentialities of Probiotics against Obesity.
Kober AKMH, Saha S, Ayyash M, Namai F, Nishiyama K, Yoda K, Villena J, Kitazawa H. Kober AKMH, et al. Nutrients. 2024 Apr 30;16(9):1373. doi: 10.3390/nu16091373. Nutrients. 2024. PMID: 38732619 Free PMC article. Review. - Association between kimchi consumption and obesity based on BMI and abdominal obesity in Korean adults: a cross-sectional analysis of the Health Examinees study.
Jung H, Yun YR, Hong SW, Shin S. Jung H, et al. BMJ Open. 2024 Jan 30;14(2):e076650. doi: 10.1136/bmjopen-2023-076650. BMJ Open. 2024. PMID: 38290970 Free PMC article. - Anti-Obesity Effect of Kimchi with Starter Cultures in 3T3-L1 Cells.
Hyun IK, Hong SW, Ma MJ, Chang JY, Lee S, Yun YR. Hyun IK, et al. J Microbiol Biotechnol. 2024 Jan 28;34(1):123-131. doi: 10.4014/jmb.2307.07005. Epub 2023 Oct 11. J Microbiol Biotechnol. 2024. PMID: 37830224 Free PMC article. - Discrimination of Dendropanax morbifera via HPLC fingerprinting and SNP analysis and its impact on obesity by modulating adipogenesis- and thermogenesis-related genes.
Awais M, Akter R, Boopathi V, Ahn JC, Lee JH, Mathiyalagan R, Kwak GY, Rauf M, Yang DC, Lee GS, Kim YJ, Jung SK. Awais M, et al. Front Nutr. 2023 Aug 2;10:1168095. doi: 10.3389/fnut.2023.1168095. eCollection 2023. Front Nutr. 2023. PMID: 37621738 Free PMC article.
References
- Park KY, Rhee SH. Functional foods from fermented vegetable products: Kimchi (Korean fermented vegetables) and functionality. In: Shi J, Ho CT, Shahidi F, editors. Asian Functional Foods. CRC Press Inc; Boca Raton, FL, USA: 2005. pp. 341–380.
- Choi WY. PhD Dissertation. Pusan National University; Busan, Korea: 2001. Development of green tea added kimchi and its chemopreventive and antiobesity effects.
- Lee KY. MS Thesis. Pusan National University; Busan, Korea: 2015. Survey on middle and high school students kimchi intake patterns and studies on the development of kimchi for adolescent.
- Cui M, Kim HY, Lee KH, Jeong JK, Hwang JH, Yeo KY, Ryu BH, Choi JH, Park KY. Antiobesity effects of kimchi in diet-induced obese mice. J Ethnic Foods. 2015;2:137–144. doi: 10.1016/j.jef.2015.08.001. - DOI
- Sheo HJ, Seo YS. The effects of dietary Chinese cabbage kimchi juice on the lipid metabolism and body weight gain in rats fed high-calories-diet. J Korean Soc Food Sci Nutr. 2004;33:91–100. doi: 10.3746/jkfn.2004.33.1.091. - DOI
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous