Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes - PubMed (original) (raw)

Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes

Kyung-Hee Lee et al. Prev Nutr Food Sci. 2015 Dec.

Abstract

The anti-obesity effects of starter (Leuconostoc mesenteroides+Lactobacillus plantarum) fermented kimchi on 3T3-L1 adipocyte were studied using naturally fermented kimchi (NK), a functional kimchi (FK, NK supplemented with green tea), and FK supplemented with added starters (FKS). Oil red O staining and cellular levels of triglyceride (TG) and glycerol were used to evaluate the in vitro anti-obesity effects of these kimchis in 3T3-L1 cells. The expressions of adipogenesis/lipogenesis-related genes of peroxisome proliferator-active receptor (PPAR)-γ, CCAAT/enhance-binding protein (C/EBP)-α, and fatty acid synthase (FAS) were determined by RT-PCR. Kimchis, especially FKS, markedly decreased TG levels and increased levels of intracellular glycerol and lipid lipolysis. In addition, FKS also reduced the mRNA levels of PPAR-γ, C/EBP-α, and FAS, which are related to adipogenesis/lipogenesis in 3T3-L1 cells. These results suggest the anti-obesity effects of FKS were to due to enhanced lipolysis and reduced adipogenesis/lipogenesis in 3T3-L1 adipocytes.

Keywords: 3T3-L1 cells; antiobesity; kimchi; starters.

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Figures

Fig. 1

Fig. 1

Effect of kimchi samples on Oil red O staining in 3T3-L1 adipocytes. Con, control; NK, naturally fermented kimchi; FK, functional kimchi; FKS, functional kimchi with starters.

Fig. 2

Fig. 2

Effect of kimchi samples on the mRNA expressions of PPAR-γ, C/EBPα, and FAS in 3T3-L1 adipocytes. The PCR products were quantified and normalized GAPDH (internal control). Band intensities were measured using a densitometer and expressed as folds versus treatment naïve controls. Fold increase=gene expression/GAPDH×control value (control fold increase=1). NK, naturally fermented kimchi; FK, functional kimchi; FKS, functional kimchi produced using prebiotic starters.

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