G6PD protects from oxidative damage and improves healthspan in mice - PubMed (original) (raw)

Figure 4. Overexpression of G6PD in vivo protects from oxidative damage.

(a,b) G6PD mRNA expression levels (a) and G6PD activity (b) in liver (left panels) and brain (right panels) of neonates, young (10–35 weeks) and old (125–143 weeks) female or male mice. For RNA measures: neonates, _n_=5 for all groups. Liver: young WT females, _n_=11; young Tg females, _n_=13; old WT females, _n_=22; old Tg females, _n_=22; young WT males, _n_=12; young Tg males, _n_=13; old WT males, _n_=13; old Tg males, _n_=11. Brain: young WT females, _n_=3; young Tg females, _n_=6; old WT females, _n_=15; old Tg females, _n_=15; young WT males, _n_=4; young Tg males, _n_=6; old WT males, _n_=6; old Tg males, _n_=5. For G6PD activity: neonates, _n_=4 for all groups. Liver: young WT females, _n_=8; young Tg females, _n_=8; old WT females, _n_=4; old Tg females, _n_=5; young WT males, _n_=4; young Tg males, _n_=4; old WT males, _n_=5; old Tg males, _n_=10. Brain: young WT females, _n_=3; young Tg females, _n_=4; old WT females, _n_=4; old Tg females, _n_=5; young WT males, _n_=4; young Tg males, _n_=4; old WT males, _n_=5; old Tg males, _n_=4. (c,d) DNA oxidation (8-OHdG adducts) in liver (c) or brain (d). (e) Lipid oxidation (MDA adducts) in liver. (f–h) GSH:GSSG ratio (f) and GSH (g) or GSSG (h) concentrations were assayed in liver samples from 2-year-old female mice. Bars represent mean±s.e.m. Statistical significance was assayed using the two-tailed Student's _t_-test: *P<0.05; **P<0.01; ***P<0.001.