Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition - PubMed (original) (raw)
. 2016 Jun;590(11):1576-85.
doi: 10.1002/1873-3468.12195. Epub 2016 May 13.
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- PMID: 27135648
- DOI: 10.1002/1873-3468.12195
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Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition
Osamu Sano et al. FEBS Lett. 2016 Jun.
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Abstract
Lysosomal protein degradation via autophagy strictly regulates cellular protein homoeostasis. Herein we performed high-content screening to identify compounds that inhibit autophagy pathways. We obtained 11 hit compounds and performed cluster analysis using cellular morphological information. Vacuolin-1, which induces the formation of giant vacuoles and is a target unknown compound, clustered with the known PIKfyve inhibitor YM201636. We further confirmed that vacuolin-1 is a potent PIKfyve inhibitor, and we finally concluded that PIKfyve inhibitors are novel chemical tools for regulating autophagy.
Keywords: PIKfyve; autophagy; vacuolin-1.
© 2016 Federation of European Biochemical Societies.
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