Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II - PubMed (original) (raw)
Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II
M F Christman et al. Cell. 1988.
Abstract
We have found that mitotic recombination within the S. cerevisiae rDNA cluster (200 tandemly repeated 9.1 kb units) is strongly suppressed and that this suppression requires the combined action of DNA topoisomerases I and II. Strains with a null mutation in the TOP1 gene (encoding topoisomerase I) or a ts mutation in the TOP2 gene (encoding topoisomerase II) grown at a semipermissive temperature show 50- to 200-fold higher frequencies of mitotic recombination in rDNA relative to TOP+ controls. Suppression of recombination is specific to the rDNA because the recombination frequency at another tandem array, the CUP1 locus, at a simple HIS4 duplication, or among dispersed repeats (MAT and HML or HMR) is not elevated in top1 or top2 mutants. The high frequency of mitotic recombination within the rDNA cluster in topoisomerase mutants shows that both TOP1 and TOP2 are required for suppression of recombination in this region of the genome.
Similar articles
- The topoisomerase II-associated protein, Pat1p, is required for maintenance of rDNA locus stability in Saccharomyces cerevisiae.
Wang X, Watt PM, Borts RH, Louis EJ, Hickson ID. Wang X, et al. Mol Gen Genet. 1999 Jun;261(4-5):831-40. doi: 10.1007/s004380050027. Mol Gen Genet. 1999. PMID: 10394921 - Failure to relax negative supercoiling of DNA is a primary cause of mitotic hyper-recombination in topoisomerase-deficient yeast cells.
Trigueros S, Roca J. Trigueros S, et al. J Biol Chem. 2002 Oct 4;277(40):37207-11. doi: 10.1074/jbc.M206663200. Epub 2002 Jul 31. J Biol Chem. 2002. PMID: 12151411 - Low levels of DNA polymerase alpha induce mitotic and meiotic instability in the ribosomal DNA gene cluster of Saccharomyces cerevisiae.
Casper AM, Mieczkowski PA, Gawel M, Petes TD. Casper AM, et al. PLoS Genet. 2008 Jun 27;4(6):e1000105. doi: 10.1371/journal.pgen.1000105. PLoS Genet. 2008. PMID: 18584028 Free PMC article. - DNA protein interactions at the rRNA of Saccharomyces cerevisiae.
Cioci F, Di Felice F, Chiani F, Camilloni G. Cioci F, et al. Ital J Biochem. 2007 Jun;56(2):81-90. Ital J Biochem. 2007. PMID: 17722648 Review. - DNA supercoiling in vivo.
Giaever GN, Snyder L, Wang JC. Giaever GN, et al. Biophys Chem. 1988 Feb;29(1-2):7-15. doi: 10.1016/0301-4622(88)87020-0. Biophys Chem. 1988. PMID: 2833949 Review.
Cited by
- Synthesis of ribosomes in Saccharomyces cerevisiae.
Warner JR. Warner JR. Microbiol Rev. 1989 Jun;53(2):256-71. doi: 10.1128/mr.53.2.256-271.1989. Microbiol Rev. 1989. PMID: 2666845 Free PMC article. Review. - Common Features of the Pericentromere and Nucleolus.
Lawrimore CJ, Bloom K. Lawrimore CJ, et al. Genes (Basel). 2019 Dec 10;10(12):1029. doi: 10.3390/genes10121029. Genes (Basel). 2019. PMID: 31835574 Free PMC article. Review. - HPR1 encodes a global positive regulator of transcription in Saccharomyces cerevisiae.
Zhu Y, Peterson CL, Christman MF. Zhu Y, et al. Mol Cell Biol. 1995 Mar;15(3):1698-708. doi: 10.1128/MCB.15.3.1698. Mol Cell Biol. 1995. PMID: 7862161 Free PMC article. - The rRNA-encoding DNA array has an altered structure in topoisomerase I mutants of Saccharomyces cerevisiae.
Christman MF, Dietrich FS, Levin NA, Sadoff BU, Fink GR. Christman MF, et al. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7637-41. doi: 10.1073/pnas.90.16.7637. Proc Natl Acad Sci U S A. 1993. PMID: 8395051 Free PMC article. - Mutations in the yeast SRB2 general transcription factor suppress hpr1-induced recombination and show defects in DNA repair.
Piruat JI, Aguilera A. Piruat JI, et al. Genetics. 1996 Aug;143(4):1533-42. doi: 10.1093/genetics/143.4.1533. Genetics. 1996. PMID: 8844143 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous