VSL#3 can prevent ulcerative colitis-associated carcinogenesis in mice - PubMed (original) (raw)

VSL#3 can prevent ulcerative colitis-associated carcinogenesis in mice

Chun-Sai-Er Wang et al. World J Gastroenterol. 2018.

Abstract

Aim: To investigate the effects of VSL#3 on tumor formation, and fecal and intestinal mucosal microbiota in azoxymethane/dextran sulfate sodium (AOM/DSS) induced mice model.

Methods: C57BL/6 mice were administered AOM/DSS to develop the ulcerative colitis (UC) carcinogenesis model. Mice were treated with 5-ASA (75 mg/kg/d), VSL#3 (1.5 × 109 CFU/d), or 5-ASA combined with VSL#3 by gavage from the day of AOM injection for three months (five days/week). The tumor load was compared in each group, and tumor necrosis factor (TNF-α) and interleukin (IL)-6 levels were evaluated in colon tissue. The stool and intestinal mucosa samples were collected to analyze the differences in the intestinal microbiota by 16s rDNA sequencing method.

Results: VSL#3 significantly reduced the tumor load in AOM/DSS-induced mice model and decreased the level of TNF-α and IL-6 in colon tissue. The model group had a lower level of Lactobacillus and higher level of Oscillibacter and Lachnoclostridium in fecal microbiota than the control group. After the intervention with 5-ASA and VSL#3, Bacillus and Lactococcus were increased, while Lachnoclostridium and Oscillibacter were reduced. 5-ASA combined with VSL#3 increased the Lactobacillus and decreased the Oscillibacter. The intestinal mucosal microbiota analysis showed a lower level of Bifidobacterium and Ruminococcaceae_UCG-014 and higher level of Alloprevotella in the model group as compared to the control group. After supplementation with VSL#3, Bifidobacterium was increased. 5-ASA combined with VSL#3 increased the level of both Lachnoclostridium and Bifidobacterium.

Conclusion: VSL#3 can prevent UC-associated carcinogenesis in mice, reduce the colonic mucosal inflammation levels, and rebalance the fecal and mucosal intestinal microbiota.

Keywords: Interleukin-6; Intestinal microbiota; Tumor necrosis factor-α; Ulcerative colitis carcinogenesis; VSL#3.

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Conflict of interest statement

Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.

Figures

Figure 1

Figure 1

Experimental protocol for ulcerative colitis-associated carcinogenesis model and treatment.

Figure 2

Figure 2

Body weight in each group.

Figure 3

Figure 3

Representative image of colonic tumor in each group that was examined under naked eye.

Figure 4

Figure 4

Representative image of hematoxylin-eosin staining of colon tissue examined under a microscope (40 × and 100 ×). A: Control group, the colonic mucosa glands were normal in the control group, the structure was regular, and the opening was good; B: Model group, the colonic gland structure presented disorder, large nuclei, deep staining, and nucleoplasmic ratio imbalance.

Figure 5

Figure 5

Tumor load in each group. a_P_ < 0.05, b_P_ < 0.01, c_P_ < 0.001.

Figure 6

Figure 6

Colonic tumor necrosis factor-α and interleukin-6 levels in different groups. a_P_ < 0.05, b_P_ < 0.01, c_P_ < 0.001. TNF-α: Tumor necrosis factor-α; IL-6: Interleukin-6.

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