Fecal microbiota and metabolites are distinct in a pilot study of pediatric Crohn's disease patients with higher levels of perceived stress - PubMed (original) (raw)

Fecal microbiota and metabolites are distinct in a pilot study of pediatric Crohn's disease patients with higher levels of perceived stress

Laura M Mackner et al. Psychoneuroendocrinology. 2020 Jan.

Abstract

Stress is associated with increased Crohn's Disease (CD) activity. This pilot study tested whether pediatric patients with CD reporting higher levels of perceived stress exhibited differences in the fecal microbiome and metabolome. The perceived stress scale (PSS) questionnaire was administered within 2 days of collecting a stool sample for microbiome (using 16S rRNA gene sequencing) and metabolome (using NMR metabolomics) analyses. Higher levels of perceived stress were correlated with increased disease activity on the short Pediatric Crohn's Disease Activity Index (sPCDAI). Patients with High PSS scores vs. Low PSS scores based on a median split had significantly lower relative abundances of Firmicutes and Anaerostipes, as well as higher relative abundances of Parabacteroides. Fecal alanine and nicotinate were also significantly different in patients with High vs. Low PSS Scores. This pilot study suggests that the fecal microbiome and metabolome differs in pediatric patients with CD and high perceived stress.

Keywords: Metabolome; Microbiome; Pediatric Crohn’s disease; Stress.

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Conflict of interest statement

Conflict of Interests

The authors do not have any conflicts of interests that affect the conduct or interpretation of this study.

Figures

Figure 1:. Crohn’s disease patients with higher perceived stress have differences in specific bacterial taxa compared to patients with lower perceived stress.

A) Crohn’s disease patients in the High PSS group had significantly lower relative abundances of Firmicutes (as determined by percentage of total sequences) (p<.05). B) Crohn’s disease patients in the High PSS group had a significant decrease in the relative abundance of Anaerostipes and a significant increase in Parabacteroides (FDR p<.05). Data show the arcsin of the square root of the proportion for each bacterial genus.

Figure 2:. NMR spectra differ in CD patients with higher vs. lower levels of perceived stress.

Superimposed 1D 1H NMR spectra of the aqueous extracts of stool samples from pediatric CD patients in the Low PSS and High PSS groups (black and red spectrum respectively).

Figure 3:. Fecal metabolites differ in CD patients with higher vs lower perceived stress.

A) Multiplicity edited HSQC of a stool aqueous extract sample, displaying one bond 1H-13C correlations; positive signals (black) for the CH3/CH carbons and negative signals (red) for the CH2 carbons. B) Relative intensities of alanine and C) nicotinate are significant different in CD patients in the High PSS group. D) Normalized relative abundance of Parabacteroides was significantly correlated with alanine intensity (p < .05). ** p and FDR<.05. *p<.05 prior to FDR correction; FDR p=.13.

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Fecal microbiota and metabolites are distinct in a pilot study of pediatric Crohn's disease patients with higher levels of perceived stress - PubMed (original) (raw)