The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs - PubMed (original) (raw)

The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs

Sina Salavati et al. BMC Vet Res. 2021.

Abstract

Background: As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce oxidative stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on oxidative stress in dogs before and after OHE. In this study, 25 mature female intact dogs were selected and randomly divided into five equal groups: Melatonin (melatonin, no surgery), OHE (no melatonin, surgery), OHE + melatonin (melatonin, surgery), anesthesia+melatonin (melatonin, sham surgery), and control (no melatonin, no surgery) groups. Melatonin (0.3 mg/Kg/day, p.o.) was administrated to the dogs in the melatonin, OHE + melatonin, and anesthesia+melatonin groups on days - 1, 0, 1, 2, and 3 (day 0 = OHE). Blood sampling was performed on days - 1, 1, 3, and 5 of the study. Blood samples were immediately transferred to the laboratory and sera were separated and stored at - 20 °C. Superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and malondialdehyde (MDA) concentrations were measured with commercial kits.

Results: The levels of SOD, GPX and CAT were significantly higher in the melatonin and anesthesia+melatonin groups compared to those of the control group at days 3 and 5. The level of antioxidant enzymes significantly decreased in the OHE group compared to that of other groups at days 3 and 5. The administration of melatonin increased the level of antioxidant enzymes in ovariohysterectomized dogs. Ovariohysterectomy significantly increased the concentration of MDA in comparison to that of other groups at day 3. Melatonin administration significantly decreased the level of MDA in melatonin, anesthetized, and ovariohysterectomized dogs at day 3.

Conclusions: Administration of melatonin on day - 1, 0, 1, 2 and 3 modulate the oxidative stress induced by OHE in dogs by increasing antioxidant enzymes concentration and decreasing MDA levels.

Keywords: Antioxidants; Dog; Melatonin; Oxidative stress; Surgery.

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Conflict of interest statement

‘The authors declare that they have no competing interests.

Figures

Fig. 1

Fig. 1

The mean ± SD concentrations of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and malondialdehyde (MDA) were compared between days of sampling in each group. Melatonin (0.3 mg/kg p.o.) was administrated on days -1, 0, 1, 2 and 3. Ovariohysterectomy and anesthesia were performed on day 0. Different letters on bars indicate significant differences between days of sampling in each group

Fig. 2

Fig. 2

The mean ± SD concentrations of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and malondialdehyde (MDA) were compared between the study groups (melatonin, ovariohysterectomy (OHE), OHE+melatonin, anesthesia+melatonin, and control) in each day of sampling. Melatonin (0.3 mg/kg p.o.) was administrated on days -1, 0, 1, 2 and 3. Ovariohysterectomy and anesthesia were performed on day 0. Different letters (a, b, c, d) on bars indicate significant differences (P < 0.05) between groups on each day of sampling

Fig. 3

Fig. 3

The schematic design of the study. Blood sampling on days -1, 1, 3, and 5 was performed before oral melatonin administration (0.3 mg/kg) on days -1, 0, 1, 3, and 5 of the study. Ovariohysterectomy (OHE) and anesthesia were performed on day 0

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