A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template - PubMed (original) (raw)
A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template
W Wickner et al. Proc Natl Acad Sci U S A. 1973 Jun.
Abstract
A new form of DNA polymerase III, termed Pol III star (Pol III(*)), has been purified to homogeneity from Escherichia coli. Pol III(*) is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III. Pol III(*) and Pol III are separable by gel filtration. Pol III(*) utilizes a duplex template containing short gaps with the same catalytic properties as Pol III. However, Pol III(*) is able to replicate long, singlestranded templates such as homopolymer chains and viral circles of M13 and varphiX174 if provided with the following: spermidine, a primer fragment, and a new protein, termed copolymerase III(*) (Copol III(*)). The latter, purified to homogeneity, has no known independent enzymatic activity and supports synthesis by Pol III(*) but not by Pol I, Pol II, or Pol III.
Similar articles
- DNA polymerase 3 star requires ATP to start synthesis on a primed DNA.
Wickner W, Kornberg A. Wickner W, et al. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3679-83. doi: 10.1073/pnas.70.12.3679. Proc Natl Acad Sci U S A. 1973. PMID: 4519657 Free PMC article. - Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.
Hurwitz J, Wickner S. Hurwitz J, et al. Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10. doi: 10.1073/pnas.71.1.6. Proc Natl Acad Sci U S A. 1974. PMID: 4589895 Free PMC article. - Ten proteins required for conversion of phiX174 single-stranded DNA to duplex form in vitro. Resolution and reconstitution.
Schekman R, Weiner JH, Weiner A, Kornberg A. Schekman R, et al. J Biol Chem. 1975 Aug 10;250(15):5859-65. J Biol Chem. 1975. PMID: 1097445 - Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaE mutant of Escherichia coli C.
Dumas LB, Miller CA. Dumas LB, et al. J Virol. 1973 Jun;11(6):848-55. doi: 10.1128/JVI.11.6.848-855.1973. J Virol. 1973. PMID: 4576516 Free PMC article. - Multienzyme systems of DNA replication.
Schekman R, Weiner A, Kornberg A. Schekman R, et al. Science. 1974 Dec 13;186(4168):987-93. doi: 10.1126/science.186.4168.987. Science. 1974. PMID: 4620044 Review.
Cited by
- Conjugational synthesis of F lac+ and Col I DNA in the presence of rifampicin and in Escherichia coli K12 mutants defective in DNA synthesis.
Wilkins BM, Hollom SE. Wilkins BM, et al. Mol Gen Genet. 1974;134(2):143-56. doi: 10.1007/BF00268416. Mol Gen Genet. 1974. PMID: 4617160 No abstract available. - Origin of replication of pBR345 plasmid DNA.
Bolivar F, Betlach MC, Heyneker HL, Shine J, Rodriguez RL, Boyer HW. Bolivar F, et al. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5265-9. doi: 10.1073/pnas.74.12.5265. Proc Natl Acad Sci U S A. 1977. PMID: 271952 Free PMC article. - Authentic reverse transcriptase is coded by jockey, a mobile Drosophila element related to mammalian LINEs.
Ivanov VA, Melnikov AA, Siunov AV, Fodor II, Ilyin YV. Ivanov VA, et al. EMBO J. 1991 Sep;10(9):2489-95. doi: 10.1002/j.1460-2075.1991.tb07788.x. EMBO J. 1991. PMID: 1714378 Free PMC article. - Enzymatic multiplication of a chemically synthesized DNA fragment.
Olson K, Gabriel T, Michalewsky J, Harvey C. Olson K, et al. Nucleic Acids Res. 1975 Jan;2(1):43-60. doi: 10.1093/nar/2.1.43. Nucleic Acids Res. 1975. PMID: 1093143 Free PMC article. - Function of DNA polymerase I in RNA-primed synthesis of bacteriophage M-13 duplex DNA.
Schneck PK, Staudenbauer WL, Hofschneider PH. Schneck PK, et al. Nucleic Acids Res. 1976 Mar;3(3):599-613. doi: 10.1093/nar/3.3.599. Nucleic Acids Res. 1976. PMID: 1272793 Free PMC article.
References
- Virology. 1971 Apr;44(1):168-87 - PubMed
- Biochem Biophys Res Commun. 1970 Sep 30;40(6):1348-55 - PubMed
- J Mol Biol. 1971 Nov 28;62(1):39-52 - PubMed
- Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9 - PubMed
- J Biol Chem. 1972 Sep 10;247(17):5369-75 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources