Microvascular basal lamina antigens disappear during cerebral ischemia and reperfusion - PubMed (original) (raw)

Microvascular basal lamina antigens disappear during cerebral ischemia and reperfusion

G F Hamann et al. Stroke. 1995 Nov.

Abstract

Background and purpose: Changes in vascular permeability are well-known and important consequences of cerebral ischemia. The development of edema and of petechial hemorrhage is connected to altered vascular integrity. A major part in microvascular integrity is played by the basal lamina.

Methods: The fates of the basal lamina components laminin, fibronectin, and type IV collagen during middle cerebral artery occlusion (2 hours, n = 3) and occlusion (3 hours) with reperfusion (1 hour, n = 3; 4 hours, n = 3; and 24 hours, n = 4) were evaluated in the nonhuman primate. Specific monoclonal antibodies against these components were used. The number and size distribution of the microvessels in each specimen were determined by video-imaging microscopy, and the relative fluorescence intensity of laminin was semiquantified by laser confocal microscopy. Basal lamina antigen presentations were compared by double-stain immunofluorescence histochemistry.

Results: The number of microvascular structures defined by the presence of each basal lamina antigen decreased significantly up to 24 hours of reperfusion (P < .0001). The ratio of laminin-containing vessels between the ischemic and nonischemic territories decreased significantly from control (0.98 +/- 0.04) to 2 hours of ischemia (0.83 +/- 0.09) and 1 hour (0.79 +/- 0.08), 4 hours (0.77 +/- 0.06), and 24 hours of reperfusion (0.55 +/- 0.07). The ratio of fibronectin (cellular) and of collagen (IV)-containing vessels decreased from 0.98 +/- 0.04 to 0.75 +/- 0.1 and from 1.02 +/- 0.03 to 0.57 +/- 0.1, respectively. Mean laminin fluorescence intensity decreased from 76.1 +/- 6.0 U (controls) to 52.0 +/- 14.6 U (24 hours of reperfusion; P < .001).

Conclusions: The significant parallel losses of three basal lamina components, both in number and intensity, contribute to loss of microvascular integrity. These phenomena may be important for understanding cell extravasation and the hemorrhagic consequences of acute stroke.

PubMed Disclaimer

Cited by

Plasminogen activators and ischemic stroke: conditions for acute delivery.
del Zoppo GJ. del Zoppo GJ. Semin Thromb Hemost. 2013 Jun;39(4):406-25. doi: 10.1055/s-0033-1338126. Epub 2013 Mar 28. Semin Thromb Hemost. 2013. PMID: 23539414 Free PMC article. Review.
Matrix metalloproteinase and cytokine profiles in monocytes over the course of stroke.
Kouwenhoven M, Carlström C, Ozenci V, Link H. Kouwenhoven M, et al. J Clin Immunol. 2001 Sep;21(5):365-75. doi: 10.1023/a:1012244820709. J Clin Immunol. 2001. PMID: 11720009
Responses of endothelial cell and astrocyte matrix-integrin receptors to ischemia mimic those observed in the neurovascular unit.
Milner R, Hung S, Wang X, Berg GI, Spatz M, del Zoppo GJ. Milner R, et al. Stroke. 2008 Jan;39(1):191-7. doi: 10.1161/STROKEAHA.107.486134. Epub 2007 Nov 21. Stroke. 2008. PMID: 18032737 Free PMC article.
Basement membrane and stroke.
Yao Y. Yao Y. J Cereb Blood Flow Metab. 2019 Jan;39(1):3-19. doi: 10.1177/0271678X18801467. Epub 2018 Sep 18. J Cereb Blood Flow Metab. 2019. PMID: 30226080 Free PMC article.
Hemostasis and alterations of the central nervous system.
del Zoppo GJ, Izawa Y, Hawkins BT. del Zoppo GJ, et al. Semin Thromb Hemost. 2013 Nov;39(8):856-75. doi: 10.1055/s-0033-1357490. Epub 2013 Oct 28. Semin Thromb Hemost. 2013. PMID: 24166247 Free PMC article. Review.

Microvascular basal lamina antigens disappear during cerebral ischemia and reperfusion - PubMed (original) (raw)