Bacterial mesosomes. Real structures or artifacts? - PubMed (original) (raw)
Bacterial mesosomes. Real structures or artifacts?
M T Silva et al. Biochim Biophys Acta. 1976.
Abstract
The ultrastructural study of membrane organization in gram-positive bacteria related to the OSO4 fixation conditions revealed that large, complex mesosomes are observed only when the bacteria are subjected to an initial fixation with 0.1%OSO4 in the culture broth, as in the prefixation step of the Ryter-Kellenberger procedure. Evidence was obtained suggesting that the large mesosomes are produced by this prefization. The kinetic study of the membrane morphological alterations occurring during the prefixation of Bacillus cereus with 0.1%OSO4 in the culture broth showed that the amount of mesosome material increases linearly from zero to a maximum observed at 1.7 min of prefixation and that at about this time a maximum is reached for the number of mesosomes per unity of cell area and for the average individual mesosome area. The large mesosomes observed in gram-positives fixed by the complete Ryter-Kellenberger procedure would be the result of the membrane-damaging action of 0.1%OSO4. Such damaging action was deduced from the observation thay 0.1%OSO4 quickly lyses protoplasts and induces a quick and extensive leakage of intracellular K+ from B. cereus and Streptococcus faecalis. In support of that interpretation is the observation that in bacteria subjected to several membrane-damaging treatments, mesosome-like structures are seen after three different fixation procedures. In bacteria initially fixed with 1% OSO4, 4% OSO4 or 2.5% glutaraldehyde, no large complex mesosomes are observed, small and simple invaginations of the cytoplasmic membrane being present. The size of these minute mesosomes is inversely proportional that causes of fixation. Uranyl acetate was found among the studied fixatives the one to the rate the least damage to bacterial membranes. This fixative satisfactorily preserves protoplasts. In bacteria initially fixed with uranyl acetate no mesosomes were found. The results of the present work throw serious doubts on the existence of mesosomes, both large and small, as real structures of bacterial cells. It is proposed that a continuous cytoplasmic membrane without infoldings (mesosomes) would be the real pattern of membrane organization in gram-positives.
Similar articles
- Bacterial mesosomes: method dependent artifacts.
Ebersold HR, Cordier JL, Lüthy P. Ebersold HR, et al. Arch Microbiol. 1981 Sep;130(1):19-22. doi: 10.1007/BF00527066. Arch Microbiol. 1981. PMID: 6796029 - Factors influencing the frequency of mesosomes observed in fixed and unfixed cells of Streptococcus faecalis.
Higgins ML, Daneo-Moore L. Higgins ML, et al. J Cell Biol. 1974 May;61(2):288-300. doi: 10.1083/jcb.61.2.288. J Cell Biol. 1974. PMID: 4208070 Free PMC article. - ELECTRON MICROSCOPE STUDY OF THE RELATIONSHIP BETWEEN MESOSOME LOSS AND THE STABLE L STATE (OR PROTOPLAST STATE) IN BACILLUS SUBTILIS.
RYTER A, LANDMAN OE. RYTER A, et al. J Bacteriol. 1964 Aug;88(2):457-67. doi: 10.1128/jb.88.2.457-467.1964. J Bacteriol. 1964. PMID: 14203364 Free PMC article. - Structure and functions of mesosomes of gram positive bacteria.
Ryter A. Ryter A. Curr Top Microbiol Immunol. 1969;49:151-77. doi: 10.1007/978-3-642-46166-8_4. Curr Top Microbiol Immunol. 1969. PMID: 4980055 Review. No abstract available. - [Bacterial mesosomes].
Hashimoto T. Hashimoto T. Tanpakushitsu Kakusan Koso. 1966 Oct;11(12):1209-16. Tanpakushitsu Kakusan Koso. 1966. PMID: 5341806 Review. Japanese. No abstract available.
Cited by
- Molecular Cytology of 'Little Animals': Personal Recollections of Escherichia coli (and Bacillus subtilis).
Nanninga N. Nanninga N. Life (Basel). 2023 Aug 21;13(8):1782. doi: 10.3390/life13081782. Life (Basel). 2023. PMID: 37629639 Free PMC article. Review. - Antibacterial activity of supernatants of Lactoccocus lactis, Lactobacillus rhamnosus, Pediococcus pentosaceus and curcumin against Aeromonas hydrophila. In vitro study.
Ibarra-Martínez D, Muñoz-Ortega MH, Quintanar-Stephano A, Martínez-Hernández SL, Ávila-Blanco ME, Ventura-Juárez J. Ibarra-Martínez D, et al. Vet Res Commun. 2022 Jun;46(2):459-470. doi: 10.1007/s11259-021-09871-7. Epub 2022 Jan 8. Vet Res Commun. 2022. PMID: 34997440 - Loose Morphology and High Dynamism of OSER Structures Induced by the Membrane Domain of HMG-CoA Reductase.
Grados-Torrez RE, López-Iglesias C, Ferrer JC, Campos N. Grados-Torrez RE, et al. Int J Mol Sci. 2021 Aug 24;22(17):9132. doi: 10.3390/ijms22179132. Int J Mol Sci. 2021. PMID: 34502042 Free PMC article. - Streptococcal Extracellular Membrane Vesicles Are Rapidly Internalized by Immune Cells and Alter Their Cytokine Release.
Mehanny M, Koch M, Lehr CM, Fuhrmann G. Mehanny M, et al. Front Immunol. 2020 Feb 14;11:80. doi: 10.3389/fimmu.2020.00080. eCollection 2020. Front Immunol. 2020. PMID: 32117243 Free PMC article. - Silver Nanoparticles Combined With Naphthoquinones as an Effective Synergistic Strategy Against Staphylococcus aureus.
Krychowiak M, Kawiak A, Narajczyk M, Borowik A, Królicka A. Krychowiak M, et al. Front Pharmacol. 2018 Jul 26;9:816. doi: 10.3389/fphar.2018.00816. eCollection 2018. Front Pharmacol. 2018. PMID: 30140226 Free PMC article.