The Cln3 cyclin is down-regulated by translational repression and degradation during the G1 arrest caused by nitrogen deprivation in budding yeast - PubMed (original) (raw)
The Cln3 cyclin is down-regulated by translational repression and degradation during the G1 arrest caused by nitrogen deprivation in budding yeast
C Gallego et al. EMBO J. 1997.
Abstract
Nutrients are among the most important trophic factors in all organisms. When deprived of essential nutrients, yeast cells use accumulated reserves to complete the current cycle and arrest in the following G1 phase. We show here that the Cln3 cyclin, which has a key role in the timely activation of SBF (Swi4-Swi6)- and MBF (Mbp1-Swi6)-dependent promoters in late G1, is down-regulated rapidly at a post-transcriptional level in cells deprived of the nitrogen source. In addition to the fact that Cln3 is degraded faster by ubiquitin-dependent mechanisms, we have found that translation of the CLN3 mRNA is repressed approximately 8-fold under nitrogen deprivation conditions. As a consequence, both SBF- and MBF-dependent expression is strongly down-regulated. Mainly because of their transcriptional dependence on SBF, and perhaps with the contribution of similar post-transcriptional mechanisms to those found for Cln3, the G1 cyclins Cln1 and 2 become undetectable in starved cells. The complete loss of Cln cyclins and the sustained presence of the Clb-cyclin kinase inhibitor Sic1 in starved cells may provide the molecular basis for the G1 arrest caused by nitrogen deprivation.
Similar articles
- Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5.
de Bruin RA, McDonald WH, Kalashnikova TI, Yates J 3rd, Wittenberg C. de Bruin RA, et al. Cell. 2004 Jun 25;117(7):887-98. doi: 10.1016/j.cell.2004.05.025. Cell. 2004. PMID: 15210110 - Rme1, which controls CLN2 expression in Saccharomyces cerevisiae, is a nuclear protein that is cell cycle regulated.
Frenz LM, Johnson AL, Johnston LH. Frenz LM, et al. Mol Genet Genomics. 2001 Nov;266(3):374-84. doi: 10.1007/s004380100515. Mol Genet Genomics. 2001. PMID: 11713667 - Starting to cycle: G1 controls regulating cell division in budding yeast.
Sherlock G, Rosamond J. Sherlock G, et al. J Gen Microbiol. 1993 Nov;139(11):2531-41. doi: 10.1099/00221287-139-11-2531. J Gen Microbiol. 1993. PMID: 8277239 Review. - Is START a switch?
Cross F, McKinney J. Cross F, et al. Ciba Found Symp. 1992;170:20-5; discussion 25-9. doi: 10.1002/9780470514320.ch3. Ciba Found Symp. 1992. PMID: 1483346 Review.
Cited by
- Modeling the START transition in the budding yeast cell cycle.
Ravi J, Samart K, Zwolak J. Ravi J, et al. PLoS Comput Biol. 2024 Aug 2;20(8):e1012048. doi: 10.1371/journal.pcbi.1012048. eCollection 2024 Aug. PLoS Comput Biol. 2024. PMID: 39093881 Free PMC article. - Control of meiotic entry by dual inhibition of a key mitotic transcription factor.
Su AJ, Yendluri SC, Ünal E. Su AJ, et al. Elife. 2024 Feb 27;12:RP90425. doi: 10.7554/eLife.90425. Elife. 2024. PMID: 38411169 Free PMC article. - Neomycin Interferes with Phosphatidylinositol-4,5-Bisphosphate at the Yeast Plasma Membrane and Activates the Cell Wall Integrity Pathway.
Jiménez-Gutiérrez E, Fernández-Acero T, Alonso-Rodríguez E, Molina M, Martín H. Jiménez-Gutiérrez E, et al. Int J Mol Sci. 2022 Sep 20;23(19):11034. doi: 10.3390/ijms231911034. Int J Mol Sci. 2022. PMID: 36232332 Free PMC article. - Mad3 modulates the G1 Cdk and acts as a timer in the Start network.
Pérez AP, Artés MH, Moreno DF, Clotet J, Aldea M. Pérez AP, et al. Sci Adv. 2022 May 6;8(18):eabm4086. doi: 10.1126/sciadv.abm4086. Epub 2022 May 6. Sci Adv. 2022. PMID: 35522754 Free PMC article. - The Cell Wall Integrity Receptor Mtl1 Contributes to Articulate Autophagic Responses When Glucose Availability Is Compromised.
Montella-Manuel S, Pujol-Carrion N, de la Torre-Ruiz MA. Montella-Manuel S, et al. J Fungi (Basel). 2021 Oct 26;7(11):903. doi: 10.3390/jof7110903. J Fungi (Basel). 2021. PMID: 34829194 Free PMC article.
References
- Science. 1993 Sep 17;261(5128):1551-7 - PubMed
- Mol Cell Biol. 1993 Sep;13(9):5843-53 - PubMed
- Cell. 1993 Sep 24;74(6):993-1007 - PubMed
- Mol Cell Biol. 1993 Oct;13(10):6274-82 - PubMed
- EMBO J. 1993 Dec 15;12(13):5267-75 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials