GMD - Pyruvic acid (1MEOX) (1TMS) (original) (raw)

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Replica Mass Spectra of Pyruvic acid (1MEOX) (1TMS)

replica lib entry date detection method species
16 30 March 2011 Fiehn_GC_2010
2 15 March 2005 MS-TOF M[6] Reference Substance
13 10 December 2010 MS-TOF VAR5
3 19 July 2007 MS-TOF VAR5 Reference Substance
15 03 November 2010 Schomburg_GC_2010
5 16 June 2005 MS-TOF M[STR]
17 28 August 2012 MassBank GC 2010 Tsujimoto
9 11 September 2005 M[FAME4090] Standard
10 12 May 2005 M[NIST]
14 07 January 2011 MS-TOF VAR5
13 spectrum(a)
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Spectrum Details

analyte Pyruvic acid (1MEOX) (1TMS)
analyte InChI InChI=1S/C7H15NO3Si/c1-6(8-10-2)7(9)11-12(3,4)5/h1-5H3/b8-6+
analyte mass 189.28
chromatogram 6058hf_53
citation
authors Boelling C, Liebig F, Erban A, Kopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
lib entry date 19 July 2007
metabolite role
retention time (sec) 208.84
retention index (VAR5 method, n-alkanes C10–C36)
base peak (m/z) 73
maximal intensity 197,160
mass-intensity-peaks cardinality 98 intensities
minimal m/z 45
maximal m/z 947
download JCAMP DX SpectrumJcampDx.ashx?id=30a45b94-ed4c-450b-ae09-030bf3562135
download MSP SpectrumMsp.ashx?id=30a45b94-ed4c-450b-ae09-030bf3562135

GC-Method

name MDN35
citation Kopka J, Max Planck Institute of Molecular Plant Physiology, Department of Molecular Plant Physiology (Prof. Willmitzer L), Am Muehlenberg 1, D-14476 Golm, Germany
attribute text details
deconvolution ChromaTOF BASELINE OFFSET: 1; SMOOTHING: 5; PEAK WIDTH: 3s; S/N: 10
derivatization MEOX; MSTFA METHOXYAMINATION: 120min at 37°C; TRIMETHYLSILYLATION: 30min at 37°C
detector MS-TOF m/z = 70-600; scans:20/s;
extraction chloroform:dH2O (2:1; v/v); chloroform (20°C); dH2O (4°C)
ion source EI 70eV
RI FAME, d6-Cholesterole FAME: C8, C9, C10, C12, C14, C16, C18, C20, C22, C24, C26, C28, C30
separation GC COLUMN:35%phenyl-65%dimethylpolysiloxane, 30m, ID:0.32mm, DF:0.25µm, MDN-35 (Macherey-Nagel, Düren, Germany); PROGRAM:iso 2min 80°C, ramp 15°C/min, iso 6min 330°C; FLOW:Helium, 2mL/min; INJECTION:1µL, splitless, 230°C; TRANSFER:250°C; IONSOURCE:250°C

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