Mahmood Ahmed | Huazhong Agriculture University (original) (raw)
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The tetraploid species Gossypium hirsutum is cultivated widely throughout the world with high yie... more The tetraploid species Gossypium hirsutum is cultivated widely throughout the world with high yield and moderate fiber quality, but its genetic basis is narrow. A set of 107 introgression lines (ILs) was developed with an interspecific cross using G. hirsutum acc. 4105 as the recurrent parent and G. tomentosum as the donor parent. A specific locus amplified fragment sequencing (SLAF-seq) strategy was used to obtain high-throughput single nucleotide polymorphism (SNP) markers. In total, 3157 high-quality SNP markers were obtained and further used for identification of quantitative trait loci (QTLs) for fiber quality and yield traits evaluated in multiple environments. In total, 74 QTLs were detected that were associated with five fiber quality traits (30 QTLs) and eight yield traits (44 QTLs), with 2.02–30.15% of the phenotypic variance explained (PVE), and 69 markers were found to be associated with these thirteen traits. Eleven chromosomes in the A sub-genome (At) harbored 47 QTLs, and nine chromosomes in the D sub-genome (Dt) harbored 27 QTLs. More than half (44 QTLs = 59.45%) showed positive additive effects for fiber and yield traits. Five QTL clusters were identified, with three in the At, comprised of thirteen QTLs, and two in the Dt comprised of seven QTLs. The ILs developed in this study and the identified QTLs will facilitate further molecular breeding for improvement of Upland cotton in terms of higher yield with enhanced fiber quality.
Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. ... more Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. The previous two decades have witnessed a combined endeavor across various biological sciences to address this issue in novel ways. The advent of recombinant DNA technologies has provided the tools for producing recombinant proteins that can be used as therapeutic agents. A number of expression systems have been developed for the production of pharmaceutical products. Recently, advances have been made using plants as bioreactors to produce therapeutic proteins directed against infectious diseases and cancers. This review highlights the recent progress in therapeutic protein expression in plants (stable and transient), the factors affecting heterologous protein expression, vector systems and recent developments in existing technologies and steps towards the industrial production of plant-made vaccines, antibodies, and biopharmaceuticals.
Precise identification of cotton cultivars and hybrids is requisite to facilitate management of g... more Precise identification of cotton cultivars and hybrids is requisite to facilitate management of germplasm resources and successful hybridization programs. Fingerprinting based on minimal core set of highly informative primers will be more enlightening to unveil genetic constitution among cotton cultivars and hybrids from distinct growing regions of china. Thirty-eight upland cotton cultivars and 55 hybrids were selected from three cotton growing regions of china mainland i.e. yellow river cotton valley (YRCV), Yangtze river cotton valley (YzRCV) and north west dry region (NWDR); featured with perceptible climatology. Twelve randomly selected cultivar and hybrids (representative sample of the three regions) were employed to reveal polymorphism across mapped SSRs. Sixty-six genome-covered polymorphic SSRs were employed to assess genetic relatedness among all accessions. The results showed a polymorphism information content range from 0.34-0.86 and resolving power 0.04-2.45 for genome-covered SSRs. Higher PIC and Rp values rendered selection of 13 highly informative potential core SSRs, whose average PIC and Rp values were 0.80 and 1.64, respectively. Moreover, Jaccard's similarity coefficients of genome-covered and potential core SSRs were compared and found to be related. Potential core SSRs substantiated clustering results of genome-covered SSRs and successfully discriminated all accession. Overall clustering pattern and revealed genetic constitution of tested material suggested that the 13 potential core SSRs could assure comparable results with higher resolution as compared to that of genome-covered SSRs.
The tetraploid species Gossypium hirsutum is cultivated widely throughout the world with high yie... more The tetraploid species Gossypium hirsutum is cultivated widely throughout the world with high yield and moderate fiber quality, but its genetic basis is narrow. A set of 107 introgression lines (ILs) was developed with an interspecific cross using G. hirsutum acc. 4105 as the recurrent parent and G. tomentosum as the donor parent. A specific locus amplified fragment sequencing (SLAF-seq) strategy was used to obtain high-throughput single nucleotide polymorphism (SNP) markers. In total, 3157 high-quality SNP markers were obtained and further used for identification of quantitative trait loci (QTLs) for fiber quality and yield traits evaluated in multiple environments. In total, 74 QTLs were detected that were associated with five fiber quality traits (30 QTLs) and eight yield traits (44 QTLs), with 2.02–30.15% of the phenotypic variance explained (PVE), and 69 markers were found to be associated with these thirteen traits. Eleven chromosomes in the A sub-genome (At) harbored 47 QTLs, and nine chromosomes in the D sub-genome (Dt) harbored 27 QTLs. More than half (44 QTLs = 59.45%) showed positive additive effects for fiber and yield traits. Five QTL clusters were identified, with three in the At, comprised of thirteen QTLs, and two in the Dt comprised of seven QTLs. The ILs developed in this study and the identified QTLs will facilitate further molecular breeding for improvement of Upland cotton in terms of higher yield with enhanced fiber quality.
Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. ... more Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. The previous two decades have witnessed a combined endeavor across various biological sciences to address this issue in novel ways. The advent of recombinant DNA technologies has provided the tools for producing recombinant proteins that can be used as therapeutic agents. A number of expression systems have been developed for the production of pharmaceutical products. Recently, advances have been made using plants as bioreactors to produce therapeutic proteins directed against infectious diseases and cancers. This review highlights the recent progress in therapeutic protein expression in plants (stable and transient), the factors affecting heterologous protein expression, vector systems and recent developments in existing technologies and steps towards the industrial production of plant-made vaccines, antibodies, and biopharmaceuticals.
Precise identification of cotton cultivars and hybrids is requisite to facilitate management of g... more Precise identification of cotton cultivars and hybrids is requisite to facilitate management of germplasm resources and successful hybridization programs. Fingerprinting based on minimal core set of highly informative primers will be more enlightening to unveil genetic constitution among cotton cultivars and hybrids from distinct growing regions of china. Thirty-eight upland cotton cultivars and 55 hybrids were selected from three cotton growing regions of china mainland i.e. yellow river cotton valley (YRCV), Yangtze river cotton valley (YzRCV) and north west dry region (NWDR); featured with perceptible climatology. Twelve randomly selected cultivar and hybrids (representative sample of the three regions) were employed to reveal polymorphism across mapped SSRs. Sixty-six genome-covered polymorphic SSRs were employed to assess genetic relatedness among all accessions. The results showed a polymorphism information content range from 0.34-0.86 and resolving power 0.04-2.45 for genome-covered SSRs. Higher PIC and Rp values rendered selection of 13 highly informative potential core SSRs, whose average PIC and Rp values were 0.80 and 1.64, respectively. Moreover, Jaccard's similarity coefficients of genome-covered and potential core SSRs were compared and found to be related. Potential core SSRs substantiated clustering results of genome-covered SSRs and successfully discriminated all accession. Overall clustering pattern and revealed genetic constitution of tested material suggested that the 13 potential core SSRs could assure comparable results with higher resolution as compared to that of genome-covered SSRs.