Adnan Jaran | Al Albayt (original) (raw)

Papers by Adnan Jaran

Research paper thumbnail of Biological Activity of the Water Soluble Complex trans-dichloro-1,2-propylenediamine-N,N,N',N'-Tetraacetato Ruthenium (III) (RAD)

Research paper thumbnail of Characterization of Ampicillin Resistant Gene (blaTEM-1) Isolated from E. coli in Northern Jordan

Asian Journal of Biomedical and Pharmaceutical Sciences, 2017

The aim of this project is to study the molecular characteristics of blaTEM-1 gene, and the assoc... more The aim of this project is to study the molecular characteristics of blaTEM-1 gene, and the associated ampicillin resistance mechanisms present in E. coli in north Jordan. In this study, 150 unrelated Escherichia coli bacterial samples were isolated from different clinical sources (urine, blood, pus and abscess) and tested for their susceptibility to 17 antimicrobial agents, including ampicillin. The isolates were typed by plasmid profiling, and were investigated by PCR for the presence of various resistance genes. Out of the 150 isolates, 14 strains were multi-resistant; they showed resistance to Cotrimoxazole (70%), Ampicillin (67%), nalidixic acid (51%), Cephalothin (27%), Augmentin and Nitrofurantoin (19%), Tetracycline and Ciprofloxacin (15%) and Gentamycin (12%). Plasmid analysis of clinical isolates showed the presence of 1 to 7 plasmids with size ranging from 1.9 to 21.1 Kb compared with the control E.coli ATCC 25922 (size range from 2 to 19.5 Kb). PCR results showed the pre...

Research paper thumbnail of Biochemical changes in the liver, kidney and serum of rat following chronic administration of cimetidine

African Journal of Biomedical Research, 2009

The effect of repeated administration of cimetidine, an antiulcer agent, twice daily for 7days on... more The effect of repeated administration of cimetidine, an antiulcer agent, twice daily for 7days on the phosphatase (acid and alkaline) and some function indices of rat liver and kidney was investigated. Sixty-four white albino rats were randomly grouped into two, A and B. Group A which consisted of 32 rats served as the control and were administered twice daily with 1ml of distilled water (the vehicle) for 7days while group B which served as the test group were also administered twice daily with 1ml of cimetidine (2.8mg.kg-1 body wt) for 7days. Cimetidine administration (2.8mg.kg-1 body wt) resulted in significant increases (P<0.05) in the activities of liver and kidney alkaline phosphatase while there was no significant change (P>0.05) in the serum alkaline phosphatase activities. Acid phosphatase showed a decreased activity (P<0.05) in the liver but an increase (P<0.05) in activity in the kidney following the administration of cimetidine. Serum albumin content exhibited significant reduction (P<0.05) throughout the experimental period. There was a decrease (P<0.05) in the concentration of serum bilirubin from after the third day which was accompanied by a significant increase (P<0.05) in creatinine concentration and these pattern of changes were sustained as long as the administration of the agent lasted. The results indicated alterations in the biochemical parameters investigated with a more pronounced effect on the liver function than the renal function tests. It can be inferred that chronic administration of cimetidine repeatedly apart from altering the activities of the phosphatase enzymes, will also adversely affect the functional capabilities of the liver and kidneys.

Research paper thumbnail of Antimicrobial Resistance Pattern and Plasmid Profile of Salmonella typhi Isolated from an Outbreak in Tehran Province

Scandinavian Journal of Infectious Diseases, 1997

... AHMAD R. BAHRMAND and ALI A. VELAYATI From the Molecular Microbiology Unit and Department of ... more ... AHMAD R. BAHRMAND and ALI A. VELAYATI From the Molecular Microbiology Unit and Department of Mycobacteriology, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran ... A. Bahvmand, PhD, Mycobacteviology Department, Pasteuv Institute Republic of Ivan ...

Research paper thumbnail of Loss Of P16/Ink4A Protein Expression Is A Common Abnormality In Hodgkin'S Lymphoma

P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss o... more P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss of P16 protein expression has been implicated in pathogenesis of many cancers, including lymphoma. Therefore, we sought to investigate if loss of P16 protein expression is associated with lymphoma and/or any specific lymphoma subtypes (Hodgkin-s lymphoma (HL) and nonHodgkin-s lymphoma (NHL)). Fifty-five lymphoma cases consisted of 30 cases of HL and 25 cases of NHL, with an age range of 3 to 78 years, were examined for loss of P16 by immunohistochemical technique using a specific antibody reacting against P16. In total, P16 loss was seen in 33% of all lymphoma cases. P16 loss was identified in 47.7% of HL cases. In contrast, only 16% of NHL showed loss of P16. Loss of P16 was seen in 67% of HL patients with 50 years of age or older, whereas P16 loss was found in only 42% of HL patients with less than 50 years of age. P16 loss in HL is somewhat higher in male (55%) than in female (30%). In...

Research paper thumbnail of 1 Incident of Pseudomonas aeruginosa in Post-Operative Wound Infection

Abstract: The primary aim of this study was to determine the incidents of pseudomonas aeruginosa ... more Abstract: The primary aim of this study was to determine the incidents of pseudomonas aeruginosa in post-operative wound infection and its sensitivity pattern to commonly used antibiotics. During a period of six months between February to December, 2005, 115 specimens were collected from King

Research paper thumbnail of Loss of P16 Protein Expression and Its Association with Epstein-Barr Virus LMP-1 Expression in Hodgkin's Lymphoma

Iranian journal of cancer prevention, 2013

Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expr... more Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma. Sections were made from archival formalin-fixed and paraffin-embedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies. In Hodgkin's Lymphoma (HL), the loss of P16 was higher in LMP-1 positive cases (61%) than LMP-1 negative cases (25%; P = 0.072). Conversely, in Non-Hodgkin's Lymphoma (NHL), none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male (75%) than female (33%). Also, there was a significantly higher p...

Research paper thumbnail of Antimicrobial resistance patterns and plasmid profiles of Staphylococcus Aureus isolated from milk and meat

East African Medical Journal, 2009

Objectives: To determine the frequency of resistance of Staphylococcus aureus to various antimicr... more Objectives: To determine the frequency of resistance of Staphylococcus aureus to various antimicrobial agents, and the relationship between antimicrobial resistance of the isolates and carriage of plasmids. Design: A random sampling of milk and meat samples was carried out. Setting: Milk was collected from various dairy cooperative societies in Nairobi and Kiambu districts. Minced meat samples were purchased from various outlets in the city of Nairobi. Subjects: Ninety six Staphylococcus aureus isolates from milk (seventy five isolates) and minced meat (twenty one isolates) samples. Main outcome measures: Plasmid profiles and antimicrobial susceptibility tests to ampicillin, lincomycin, penicillin, erythromycin, methicillin, minocycline, cotrimoxazole and chloramphenicol. Results: Seventy one per cent of the isolates carried between one and six plasmids of molecular sizes ranging from 0.1 to 14.5 kilobases. High frequency of resistance was observed with lincomycin (67.7%), penicillin (66.7%) and cotrimoxazole (51%). A high percentage (76%) of isolates were susceptible to minocycline followed by erythromycin (57.3%). Most (80.2%) of isolates were multiply resistant to between two and six antibiotics. Conclusion: Most Staphylococcus aureus isolates were multiply resistant to various antimicrobial agents, but there was no apparent relationship between carriage of plasmids and antimicrobial resistance. Milk and meat may contain resistant Staphylococcus aureus posing a potential risk to consumers.

Research paper thumbnail of Prevalence and seasonal variation of human intestinal parasites in patients attending hospital with abdominal symptoms in northern Jordan

Eastern Mediterranean Health Journal, 2016

This study was carried out to determine the prevalence of intestinal parasites and their seasonal... more This study was carried out to determine the prevalence of intestinal parasites and their seasonal variation in northern Jordan. A total of 21 906 stool samples were collected over a period of 4 years (2009-2013) from 5 government hospitals in 3 cities. Samples were processed and examined microscopically and by concentration methods. Parasitic infection was found in 9611 samples (44%). Giardia lamblia was the most prevalent parasite (41%) followed by Entamoeba histolytica (31%) and Ent. coli (13%); the least prevalent parasites were Ascaris lumbricoides (1%), Hymenolepis nana, Taenia sp., and Chilomastix mesnili (all < 1%). The prevalence of different parasites varied according to season, on average the summer months showed the highest incidence of parasitic infection (62%) compared with the winter months (16%). Giardia lamblia and Ent. histolytica were most prevalent in the summer months.

Research paper thumbnail of Antibiotic Resistance Patterns and Plasmid Profiles of Escherichia coli Isolates from Clinical Specimens

British Microbiology Research Journal, 2016

Research paper thumbnail of Molecular study on methicillin-resistant Staphylococcus aureus strains isolated from dogs and associated personnel in Jordan

Asian Pacific Journal of Tropical Biomedicine, 2015

Research paper thumbnail of Abnormal antibiotic accumulation by cystic fibrosis cells

Research paper thumbnail of Antibiotic accumulation and membrane trafficking in cystic fibrosis cells

Journal of Antimicrobial Chemotherapy, 1998

Cystic fibrosis (CF) results from mutations in the gene encoding the CF transmembrane conductance... more Cystic fibrosis (CF) results from mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) which is a regulated chloride channel. The F508 mutation prevents the post-translational glycosylation and membrane insertion of the protein. Severe disease follows, with the formation of a viscous mucus and subsequent chronic bacterial infection of the lungs, necessitating frequent, and often long, periods of antibiotic treatment. The pharmacokinetics of antibiotics in CF patients are abnormal, with lower blood serum levels and higher clearance rates which have never been satisfactorily explained. We found that accumulation of gentamicin in nasal polyp tissue non-CF cells was subject to regulation by the effectors and inhibitors of CFTR function; regulation was lost in F508 CF cells and accumulation was more than doubled because of the inhibition of exocytosis. †Present address:

Research paper thumbnail of Species Specificity Using Fifteen PCR-based Human STR Systems

Journal of Biological Sciences, 2006

Research paper thumbnail of Loss of P16/INK4A Protein Expression is a Common Abnormality in Hodgkin's Lymphoma

P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss o... more P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss of P16 protein expression has been implicated in pathogenesis of many cancers, including lymphoma. Therefore, we sought to investigate if loss of P16 protein expression is associated with lymphoma and/or any specific lymphoma subtypes (Hodgkin's lymphoma (HL) and nonHodgkin's lymphoma (NHL)). Fifty-five lymphoma cases consisted of 30 cases of HL and 25 cases of NHL, with an age range of 3 to 78 years, were examined for loss of P16 by immunohistochemical technique using a specific antibody reacting against P16. In total, P16 loss was seen in 33% of all lymphoma cases. P16 loss was identified in 47.7% of HL cases. In contrast, only 16% of NHL showed loss of P16. Loss of P16 was seen in 67% of HL patients with 50 years of age or older, whereas P16 loss was found in only 42% of HL patients with less than 50 years of age. P16 loss in HL is somewhat higher in male (55%) than in female (...

Research paper thumbnail of prevalence of Epestein -Barr Virus latent membrane protein-1 in Jordanian Patients with Hodgkin's Lymphoma and Non-Hodgkin's Lymphoma

Journal of Biological Sciences

Research paper thumbnail of Species Specificity Using Fifteen PCR-based Human STR Systems

Journal of Biological Sciences, 2006

Research paper thumbnail of Prevalence of Epstein-Barr Virus Latent Membrane Protein-1 in Jordanian Patients with Hodgkin’s Lymphoma and Non-Hodgkin’s Lymphoma

Journal of Biological Sciences, 2010

Research paper thumbnail of Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea)

The present work quantitatively evaluates the antimicrobial and antioxidant potentials of two Lib... more The present work quantitatively evaluates the antimicrobial and antioxidant potentials of two Libyan folk medicinal plants [Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea)] that commonly grow in the south of Libya. The crude methanolic leaves extracts of both plants are appreciably active against Gram-positive species, associated with week anti-Gram-negative activity. These two plant extracts also showed reasonably high contents of polyphenolics and alkaloids, with minimal inhibitory concentrations between 12.5-25 and 250-1000 lg/ml for Gram-positive and Gram-negative species, respectively. Results collectively suggest that A. campestris and Z. lotus are not only reliable natural sources of antimicrobials but also potential sources of phenolic antioxidants and hence could be nominated for future intensive studies.

Research paper thumbnail of Loss of P16 Protein Expression and Its Association with Epstein-Barr Virus LMP-1 Expression in Hodgkin's Lymphoma

Iranian journal of cancer prevention, 2013

Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expr... more Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma. Sections were made from archival formalin-fixed and paraffin-embedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies. In Hodgkin's Lymphoma (HL), the loss of P16 was higher in LMP-1 positive cases (61%) than LMP-1 negative cases (25%; P = 0.072). Conversely, in Non-Hodgkin's Lymphoma (NHL), none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male (75%) than female (33%). Also, there was a significantly higher p...

Research paper thumbnail of Biological Activity of the Water Soluble Complex trans-dichloro-1,2-propylenediamine-N,N,N',N'-Tetraacetato Ruthenium (III) (RAD)

Research paper thumbnail of Characterization of Ampicillin Resistant Gene (blaTEM-1) Isolated from E. coli in Northern Jordan

Asian Journal of Biomedical and Pharmaceutical Sciences, 2017

The aim of this project is to study the molecular characteristics of blaTEM-1 gene, and the assoc... more The aim of this project is to study the molecular characteristics of blaTEM-1 gene, and the associated ampicillin resistance mechanisms present in E. coli in north Jordan. In this study, 150 unrelated Escherichia coli bacterial samples were isolated from different clinical sources (urine, blood, pus and abscess) and tested for their susceptibility to 17 antimicrobial agents, including ampicillin. The isolates were typed by plasmid profiling, and were investigated by PCR for the presence of various resistance genes. Out of the 150 isolates, 14 strains were multi-resistant; they showed resistance to Cotrimoxazole (70%), Ampicillin (67%), nalidixic acid (51%), Cephalothin (27%), Augmentin and Nitrofurantoin (19%), Tetracycline and Ciprofloxacin (15%) and Gentamycin (12%). Plasmid analysis of clinical isolates showed the presence of 1 to 7 plasmids with size ranging from 1.9 to 21.1 Kb compared with the control E.coli ATCC 25922 (size range from 2 to 19.5 Kb). PCR results showed the pre...

Research paper thumbnail of Biochemical changes in the liver, kidney and serum of rat following chronic administration of cimetidine

African Journal of Biomedical Research, 2009

The effect of repeated administration of cimetidine, an antiulcer agent, twice daily for 7days on... more The effect of repeated administration of cimetidine, an antiulcer agent, twice daily for 7days on the phosphatase (acid and alkaline) and some function indices of rat liver and kidney was investigated. Sixty-four white albino rats were randomly grouped into two, A and B. Group A which consisted of 32 rats served as the control and were administered twice daily with 1ml of distilled water (the vehicle) for 7days while group B which served as the test group were also administered twice daily with 1ml of cimetidine (2.8mg.kg-1 body wt) for 7days. Cimetidine administration (2.8mg.kg-1 body wt) resulted in significant increases (P<0.05) in the activities of liver and kidney alkaline phosphatase while there was no significant change (P>0.05) in the serum alkaline phosphatase activities. Acid phosphatase showed a decreased activity (P<0.05) in the liver but an increase (P<0.05) in activity in the kidney following the administration of cimetidine. Serum albumin content exhibited significant reduction (P<0.05) throughout the experimental period. There was a decrease (P<0.05) in the concentration of serum bilirubin from after the third day which was accompanied by a significant increase (P<0.05) in creatinine concentration and these pattern of changes were sustained as long as the administration of the agent lasted. The results indicated alterations in the biochemical parameters investigated with a more pronounced effect on the liver function than the renal function tests. It can be inferred that chronic administration of cimetidine repeatedly apart from altering the activities of the phosphatase enzymes, will also adversely affect the functional capabilities of the liver and kidneys.

Research paper thumbnail of Antimicrobial Resistance Pattern and Plasmid Profile of Salmonella typhi Isolated from an Outbreak in Tehran Province

Scandinavian Journal of Infectious Diseases, 1997

... AHMAD R. BAHRMAND and ALI A. VELAYATI From the Molecular Microbiology Unit and Department of ... more ... AHMAD R. BAHRMAND and ALI A. VELAYATI From the Molecular Microbiology Unit and Department of Mycobacteriology, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran ... A. Bahvmand, PhD, Mycobacteviology Department, Pasteuv Institute Republic of Ivan ...

Research paper thumbnail of Loss Of P16/Ink4A Protein Expression Is A Common Abnormality In Hodgkin'S Lymphoma

P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss o... more P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss of P16 protein expression has been implicated in pathogenesis of many cancers, including lymphoma. Therefore, we sought to investigate if loss of P16 protein expression is associated with lymphoma and/or any specific lymphoma subtypes (Hodgkin-s lymphoma (HL) and nonHodgkin-s lymphoma (NHL)). Fifty-five lymphoma cases consisted of 30 cases of HL and 25 cases of NHL, with an age range of 3 to 78 years, were examined for loss of P16 by immunohistochemical technique using a specific antibody reacting against P16. In total, P16 loss was seen in 33% of all lymphoma cases. P16 loss was identified in 47.7% of HL cases. In contrast, only 16% of NHL showed loss of P16. Loss of P16 was seen in 67% of HL patients with 50 years of age or older, whereas P16 loss was found in only 42% of HL patients with less than 50 years of age. P16 loss in HL is somewhat higher in male (55%) than in female (30%). In...

Research paper thumbnail of 1 Incident of Pseudomonas aeruginosa in Post-Operative Wound Infection

Abstract: The primary aim of this study was to determine the incidents of pseudomonas aeruginosa ... more Abstract: The primary aim of this study was to determine the incidents of pseudomonas aeruginosa in post-operative wound infection and its sensitivity pattern to commonly used antibiotics. During a period of six months between February to December, 2005, 115 specimens were collected from King

Research paper thumbnail of Loss of P16 Protein Expression and Its Association with Epstein-Barr Virus LMP-1 Expression in Hodgkin's Lymphoma

Iranian journal of cancer prevention, 2013

Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expr... more Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma. Sections were made from archival formalin-fixed and paraffin-embedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies. In Hodgkin's Lymphoma (HL), the loss of P16 was higher in LMP-1 positive cases (61%) than LMP-1 negative cases (25%; P = 0.072). Conversely, in Non-Hodgkin's Lymphoma (NHL), none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male (75%) than female (33%). Also, there was a significantly higher p...

Research paper thumbnail of Antimicrobial resistance patterns and plasmid profiles of Staphylococcus Aureus isolated from milk and meat

East African Medical Journal, 2009

Objectives: To determine the frequency of resistance of Staphylococcus aureus to various antimicr... more Objectives: To determine the frequency of resistance of Staphylococcus aureus to various antimicrobial agents, and the relationship between antimicrobial resistance of the isolates and carriage of plasmids. Design: A random sampling of milk and meat samples was carried out. Setting: Milk was collected from various dairy cooperative societies in Nairobi and Kiambu districts. Minced meat samples were purchased from various outlets in the city of Nairobi. Subjects: Ninety six Staphylococcus aureus isolates from milk (seventy five isolates) and minced meat (twenty one isolates) samples. Main outcome measures: Plasmid profiles and antimicrobial susceptibility tests to ampicillin, lincomycin, penicillin, erythromycin, methicillin, minocycline, cotrimoxazole and chloramphenicol. Results: Seventy one per cent of the isolates carried between one and six plasmids of molecular sizes ranging from 0.1 to 14.5 kilobases. High frequency of resistance was observed with lincomycin (67.7%), penicillin (66.7%) and cotrimoxazole (51%). A high percentage (76%) of isolates were susceptible to minocycline followed by erythromycin (57.3%). Most (80.2%) of isolates were multiply resistant to between two and six antibiotics. Conclusion: Most Staphylococcus aureus isolates were multiply resistant to various antimicrobial agents, but there was no apparent relationship between carriage of plasmids and antimicrobial resistance. Milk and meat may contain resistant Staphylococcus aureus posing a potential risk to consumers.

Research paper thumbnail of Prevalence and seasonal variation of human intestinal parasites in patients attending hospital with abdominal symptoms in northern Jordan

Eastern Mediterranean Health Journal, 2016

This study was carried out to determine the prevalence of intestinal parasites and their seasonal... more This study was carried out to determine the prevalence of intestinal parasites and their seasonal variation in northern Jordan. A total of 21 906 stool samples were collected over a period of 4 years (2009-2013) from 5 government hospitals in 3 cities. Samples were processed and examined microscopically and by concentration methods. Parasitic infection was found in 9611 samples (44%). Giardia lamblia was the most prevalent parasite (41%) followed by Entamoeba histolytica (31%) and Ent. coli (13%); the least prevalent parasites were Ascaris lumbricoides (1%), Hymenolepis nana, Taenia sp., and Chilomastix mesnili (all < 1%). The prevalence of different parasites varied according to season, on average the summer months showed the highest incidence of parasitic infection (62%) compared with the winter months (16%). Giardia lamblia and Ent. histolytica were most prevalent in the summer months.

Research paper thumbnail of Antibiotic Resistance Patterns and Plasmid Profiles of Escherichia coli Isolates from Clinical Specimens

British Microbiology Research Journal, 2016

Research paper thumbnail of Molecular study on methicillin-resistant Staphylococcus aureus strains isolated from dogs and associated personnel in Jordan

Asian Pacific Journal of Tropical Biomedicine, 2015

Research paper thumbnail of Abnormal antibiotic accumulation by cystic fibrosis cells

Research paper thumbnail of Antibiotic accumulation and membrane trafficking in cystic fibrosis cells

Journal of Antimicrobial Chemotherapy, 1998

Cystic fibrosis (CF) results from mutations in the gene encoding the CF transmembrane conductance... more Cystic fibrosis (CF) results from mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) which is a regulated chloride channel. The F508 mutation prevents the post-translational glycosylation and membrane insertion of the protein. Severe disease follows, with the formation of a viscous mucus and subsequent chronic bacterial infection of the lungs, necessitating frequent, and often long, periods of antibiotic treatment. The pharmacokinetics of antibiotics in CF patients are abnormal, with lower blood serum levels and higher clearance rates which have never been satisfactorily explained. We found that accumulation of gentamicin in nasal polyp tissue non-CF cells was subject to regulation by the effectors and inhibitors of CFTR function; regulation was lost in F508 CF cells and accumulation was more than doubled because of the inhibition of exocytosis. †Present address:

Research paper thumbnail of Species Specificity Using Fifteen PCR-based Human STR Systems

Journal of Biological Sciences, 2006

Research paper thumbnail of Loss of P16/INK4A Protein Expression is a Common Abnormality in Hodgkin's Lymphoma

P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss o... more P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss of P16 protein expression has been implicated in pathogenesis of many cancers, including lymphoma. Therefore, we sought to investigate if loss of P16 protein expression is associated with lymphoma and/or any specific lymphoma subtypes (Hodgkin's lymphoma (HL) and nonHodgkin's lymphoma (NHL)). Fifty-five lymphoma cases consisted of 30 cases of HL and 25 cases of NHL, with an age range of 3 to 78 years, were examined for loss of P16 by immunohistochemical technique using a specific antibody reacting against P16. In total, P16 loss was seen in 33% of all lymphoma cases. P16 loss was identified in 47.7% of HL cases. In contrast, only 16% of NHL showed loss of P16. Loss of P16 was seen in 67% of HL patients with 50 years of age or older, whereas P16 loss was found in only 42% of HL patients with less than 50 years of age. P16 loss in HL is somewhat higher in male (55%) than in female (...

Research paper thumbnail of prevalence of Epestein -Barr Virus latent membrane protein-1 in Jordanian Patients with Hodgkin's Lymphoma and Non-Hodgkin's Lymphoma

Journal of Biological Sciences

Research paper thumbnail of Species Specificity Using Fifteen PCR-based Human STR Systems

Journal of Biological Sciences, 2006

Research paper thumbnail of Prevalence of Epstein-Barr Virus Latent Membrane Protein-1 in Jordanian Patients with Hodgkin’s Lymphoma and Non-Hodgkin’s Lymphoma

Journal of Biological Sciences, 2010

Research paper thumbnail of Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea)

The present work quantitatively evaluates the antimicrobial and antioxidant potentials of two Lib... more The present work quantitatively evaluates the antimicrobial and antioxidant potentials of two Libyan folk medicinal plants [Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea)] that commonly grow in the south of Libya. The crude methanolic leaves extracts of both plants are appreciably active against Gram-positive species, associated with week anti-Gram-negative activity. These two plant extracts also showed reasonably high contents of polyphenolics and alkaloids, with minimal inhibitory concentrations between 12.5-25 and 250-1000 lg/ml for Gram-positive and Gram-negative species, respectively. Results collectively suggest that A. campestris and Z. lotus are not only reliable natural sources of antimicrobials but also potential sources of phenolic antioxidants and hence could be nominated for future intensive studies.

Research paper thumbnail of Loss of P16 Protein Expression and Its Association with Epstein-Barr Virus LMP-1 Expression in Hodgkin's Lymphoma

Iranian journal of cancer prevention, 2013

Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expr... more Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP-1) and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma. Sections were made from archival formalin-fixed and paraffin-embedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies. In Hodgkin's Lymphoma (HL), the loss of P16 was higher in LMP-1 positive cases (61%) than LMP-1 negative cases (25%; P = 0.072). Conversely, in Non-Hodgkin's Lymphoma (NHL), none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male (75%) than female (33%). Also, there was a significantly higher p...