Olajide Adedayo A O Ajayi | Adekunle Ajasin University akungba akoko ondo (original) (raw)

Papers by Olajide Adedayo A O Ajayi

American-Eurasian Journal of Agricultural and Environmental Science, 2013

There are different forms of diseases that affect our aquaculture products, especially fish, adve... more There are different forms of diseases that affect our aquaculture products, especially fish, adversely. This study shows the trend on the use of antibiotics in control of aquaculture diseases so as to protect aquaculture environment and humans from diseases. Pond water samples were collected in triplicates from selected fishpond water sources in Akungba-Akoko community, Nigeria for bacteriological analysis. Mueller Hinton agar and Nutrient Agar were generally used during the study. The antibiotic sensitivity test was done using a modified Bauer-Kirby agar diffusion method. The antibiotics used during the study were Cloxacillin (CXC) 5µg, Chloramphenicol (CHL) 10µg, Tetracycline (TET) 10µg, Erythromycin (ERY) 5µg, Cotrimoxazole (COT) 25µg, Nitrofurantoin (NIT) 100µg, Ciprofloxacin (CIP) 5µg, Ampicillin (AMP) 10µg, Norfloxacin (norbactin) (NOR) 10µg, Gentamycin (GEN) 10 µg, Augmentin (AUG) 30µg, Ofloxacin (OFL) 5 µg, Cefuroxime (CFX) 30µg and Streptomycin (STR) 10 µg. Appropriate cult...

Journal of Microbiology and Antimicrobials, Feb 28, 2010

Journal of Medicinal Plants Studies, 2016

Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant a... more Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant and one of the oldest known African medicinal plants. The purpose of this research is to determine the antimicrobial potency, phytochemical activities, nutrients and elemental composition of the leaf and bark of Erythrina senegalensis. The organisms used for the study are Escherichia coli, staphylococcus aureus, Candida albicans and Klebsiella pneumonia. The leaf and bark were extracted using one extracting solvent (ethyl acetate) and three extracting solvent (N-hexane, ethyl acetate and ethanol) for elution. Erythrina senegalensis extracts were partially purified using the column chromatography method. The antimicrobial potency of the partially purified extracts of Erythrina senegelensis was determined using the agar dilution method. Quantitative and quantitative phytochemical screening of Erythrina senegelensis were also observed and recorded accordingly. The antimicrobial activity of E...

International Journal of Advances in Chemical Engineering and Biological Sciences, 2015

This study shows the effect of low frequency (Lf) magnetic field on microorganisms obtained from ... more This study shows the effect of low frequency (Lf) magnetic field on microorganisms obtained from urine contaminated soil sources in Akungba-Akoko community, Nigeria. Thirteen (13) microorganisms isolated from these sources include ten (10) bacteria isolates and three (3) fungi isolates. They were identified as Bacillus subtilis, Bacillus cereus, Clostridium tetani, Clostridium sporoene, Enterobacter cloacae, Micrococcus spp., Staphyloccocus aureus, Proteus mirabilis and Pseudomonas aeriginosa while the fungi isolates were Saccharomyces cerevisae and Rhizopus spp. The microorganisms were treated with Lf magnetic field (50mG, 100mG, 250mG, 500mG, and 1000mG) at constant time (30minutes). Increase in the intensity of the field during the study show a decrease in microbial population some isolates determined by their coliform forming unit while there is increase in some instances. This may mean an initiation of growth abilities in some microorganisms and elimination in the pathogenic ones. The antibiotic susceptibility was determined by using the Kirby-bauer disc diffusion technique. The isolates that are resistant to antibiotics are also exposed to electromagnetic field for 45 minutes and were tested again for their susceptibility pattern. They thereafter show tangible susceptibility responses to the antibiotics used. Therefore resistant bacteria could be susceptible to antibiotic by exposure of the patients or source of environmental contaminants to the Lf magnetic field. Similarly, some of the strains killed or attenuated during the process can be used for vaccine formulation.

Applied Science Reports, 2016

British Microbiology Research Journal, 2016

Journal of Food Agriculture and Environment, 2013

This study shows the complexity and distribution pattern of different types of fungal species iso... more This study shows the complexity and distribution pattern of different types of fungal species isolated from designated aquatic zones in Akungba-Akoko community, Nigeria, during rainy season. Potato Dextrose Agar medium (P.D.A) was routinely used for culturing in the laboratory. Lacto phenol cotton blue stain was used to examine the fungal isolates under x10 and x40 objective lens of the microscope amongst other standard microbiological techniques for identification purposes. Twenty-five (25) fungal species belonging to the genera Penicillium, Aspergillus, Candida, Mucor,,Rhizopus,, Absidia, and Rhizomucos. While in soil, twenty-three (23) mold species belonging to the Fusarium,Penicillium,,Chaetomium, Trichophyton, Verticillium, Acremonium, Scopulariopsis, Madurella, ,Epicoccum,,Epidermophyton, Cladosporium,genera and yeasts genera including Rhodoturula, Absidia,and Geotrichum. These genera of fungi are known as field and storage fungi. Some physico-chemical parameters evaluated shows that the pH of the water sample sources range from pH 5.7 in slow moving water to pH 6.5 in fast moving water. The lowest moisture and organic content of the 1% was obtained for runway soil sources. The soil samples from stream and well sources had the highest moisture content of 18.22% and organic content of 18.32% respectively. This study shows that both harmful and beneficial groups of fungi appear ubiquitous in the water bodies and are of potential importance in industry. . Keywords: Aquatic, Fungi, Microbiology, Water, Soil, Survey.

Journal of Toxicology and Environmental Health Sciences, 2011

A comparative study was carried out to determine the quality of three water sources: borehole, we... more A comparative study was carried out to determine the quality of three water sources: borehole, well and stream in Akungba-Akoko, Ondo State. The water sources were assessed for microbiological quality and physico-chemical properties (temperature, odor, color and pH). The stream had the highest plate count of 40 × 105 cfu/ml, while those of borehole and well water had the least of 12 × 105 cfu/ml and 14 × 105 cfu/ml respectively. The isolated organisms were identified as Escherichia coli, Staphylococcus sp., Bacillus sp., Streptococcus sp., Klebsiella sp., Pseudomonas sp., Flavobacterium sp., Enterobacter sp., Proteus sp. andPseudomonas sp. The most probable number (MPN) of the water sample sources ranged from 8 to 120 coliforms per 100 ml, which signified undesirable level of water pollution in the area covered. Water samples from the boreholes had a coliform range of 32 to 38 × 100 cfu/ml, and were adjudged to be less prone to contamination and potable than the well and stream sources, which recorded relatively higher coliform load of 44 to 70 × 100 cfu/ml. Key words: Bacteriology, borehole, stream, well, water assessment.

British Microbiology Research Journal, 2012

Aim: To determine the microbiological quality of catfish meant for public consumption in the univ... more Aim: To determine the microbiological quality of catfish meant for public consumption in the university community, Akungba-Akoko. Study design: Cross-sectional study. Place and Duration of Study: Department of Microbiology, Adekunle Ajasin University, P.M.B. 01, Akungba-Akoko, Nigeria, between May 2010 and June 2011. Methodology: Fresh catfish, Claria gariepinus, sample obtained from typical fish pond in Akungba-Akoko was subjected to microbiological Investigation in the Laboratory. Nutrient Agar, Eosine Methylene Blue Agar and Man Rogosa Sharpe Agar were generally used for isolation and maintenance of cultures during the study. Moreover a pour plate technique was used for the estimation of the total bacterial and coliform counts. Results: The total plate count of fish skin samples gave high bacterial count of 65 x 10 2 cfu/ml, the coliforms count was 7.0 x 10 1 cfu/ml, while the anaerobic organisms encountered gave a value of 20 x 10 1 cfu/ml. Similarly, bacterial count of 2.25 x 10 7 cfu/ml coliform count of 1.35 x 10 4 cfu/ml and 6.5 x 10 4 cfu/ml anaerobic organisms were obtained from gills. The isolated bacteria species identified were Bacillus spp, Staphylococcus spp, Streptococcus spp, Microcococcus spp, and members of enterobacteriaceae which include Escherichia coli and Klebsiella spp were found in the skin of the fresh fish. Other complex forms of bacterial species were also encountered in the gills of catfish sample used for this study. This includes S. aureus, E. coli, Bacillus spp. The total aerobic counts of the Clarias gariepinus (Catfish) sample were determined and the results of this study shows that the largest numbers of anaerobic microbes were found in gills. Conclusion: The study suggests adequate monitoring of our fish ponds with a view of adding some antibiotics to their feeds to reduce infectious agents from this source. Research Article British Microbiology Research Journal, 2(1): 1-9, 2012 2

British Microbiology Research Journal, 2013

Aims: To determine the bacteriological quality and geophysical abiotic components including miner... more Aims: To determine the bacteriological quality and geophysical abiotic components including mineral elements of public drinking water sources in Akungba-Akoko community located in South West Nigeria. Study Design: Water and soil samples were collected from selected ground water (e.g., well, borehole) and surface water (e.g., streams) in 20 various locations of Akungba-Akoko community. Similarly, Geographical positioning system (GPS) of the sampling site was determined. Methodology: Total bacteria and coliform content of water samples were enumerated using the pour plate technique. The physico-chemical parameters such as pH, turbidity and temperature and mineral elements constituents were determined. Total bacterial count, phosphorus (P) and copper (Cu) were also determined in the soil samples. Results: The total bacterial count ranged from as low as 1.0 x 10 cfu/mL in GLAS site to 1.22 x 10 cfu/mL in sample site GLA9, while the coliforms count ranged from 5.0 x 10 cfu/mL in well water of sample site GLG1 to 36 x 10 cfu/mL and 3.8 x 10 cfu/mL in sample site GL9 and GL7 respectively. As for soil sources, the total heterotrophic bacterial count range was from 1.8 x10 cfu/g to 8.7 x10 cfu/g. Total hardness of the water sources ranged from 4.46 ppm in sample site GLA2 to 216.86 ppm in well water (GLWS 6) in Akungba. High levels of lead in some areas as in Araromi pond zone (GLCW16) and Well water 2 Market (GLBWS 6) among others, exceeded the maximum permissible level of 0.10 mg/L. The pH Research Article British Microbiology Research Journal, 3(1): 58-72, 2013 59 of water sources range from pH 5.52 to pH 7.91 while temperature ranges from 23oC to 28oC. Conclusion: This study shows that many sources of public drinking water supply in Akungba-Akoko are microbiologically substandard with possible infiltration of some chemical contaminants. Hence, routine monitoring and protection of the water resources is necessary in this community to improve the quality of drinking water and avoid possible associated health risks.

Open Journal of Social Sciences, 2014

Jundishapur Journal of Microbiology, 2014

Background: Many disease conditions including Staphylococcal infections are becoming increasingly... more Background: Many disease conditions including Staphylococcal infections are becoming increasingly difficult to treat in South Africa due to the surge of vancomycin-oxacillin resistant strains. How widespread this phenomenon is in commensal isolates in the Nkonkobe municipality in the Eastern Cape Province of South Africa is not known, and considering the high level of immunocompromised individuals in the province, this study couldn't have come at a better time. Objectives: The objective of this study is to evaluate the prevalence of vancomycin and oxacillin co-resistance in methicillin-resistant commensal staphylococci in Nkonkobe municipality, South Africa as part of our larger study on the surveillance of reservoirs of antibiotic resistance in South Africa. Materials and Methods: Staphylococcus species were isolated from domestic animals of Nkonkobe municipality, in the Eastern Cape Province of South Africa. The isolates were evaluated for antibiotic susceptibility against a panel of several relevant antibiotics. Specific primer sets were also used for the polymerase chain reaction assay to detect the presence of mecA gene as well as vanA and vanB genes in the genome of resistant Staphylococcus species. Results: A total of 120 Staphylococcus isolates were screened, out of which, 32 (26%) were susceptible to both methicillin and vancomycin, while 12 (10%) had co-resistance to the antibiotics, which is still on the high side, both clinically and epidemiologically. Gentamicin (an aminoglycoside) had a relatively high potency against the isolates with 107 (89.17%) of the bacteria being susceptible to it, while 10 (8.33%) were resistant. On the other hand, erythromycin (a macrolide) was active against 72 (60%) of the isolates, while 5 (4.17%) and 74 (61.67%) of them yielded intermediate and resistant responses, respectively. Similarly, 51 (42.5%) of the isolates were susceptible to rifampicin, while 1 (0.83%) and 17 (14.17%) were intermediate and resistant, respectively. Conclusions: Ten percent of the isolates were positive for mecA gene among the vancomycin-oxacillin resistant strains, while van gene was not detected in any of the isolates. The data obtained would be useful in clinical control of resistant staphylococcal strains.

Scientific Research and …, 2008

American Journal of Experimental Agriculture, 2013

The purified β-amylase had more enzymatic activity than crude samples from Bacillus species as sh... more The purified β-amylase had more enzymatic activity than crude samples from Bacillus species as shown in this study whereby the activity of crude enzyme from Bacillus subtilis (WBS) and Bacillus licheniformis (WBL) were 6.24 and 4.2 unit/ml while the purified enzymes had an improved activity of 18 and 18.60 unit/ml, respectively. The protein concentration of the enzyme samples ranged from 264.639 μg in Bacillus macerans (MBM) to 627.627 μg in B. subtilis (WBS) enzyme filtrates and relatively lower values of 6.418 μg in B. licheniformis (WBL) to 77.702 μg in B.coagulans (MBC) was observed for the purified enzymes samples. Similarly, the purification process improves the specific activity of the enzyme samples during the study. The crude enzyme samples precipitated by salting with known quantity of Ammonium sulphate ((NH4)2SO4) at the range of 50 and 80% fraction improved the activity of the enzyme samples whereby the strains of B. subtilis (WBS) and B. licheniformis (WBL) had their sp...

American-Eurasian Journal of …, 2009

Microbes and mineral constituents of oil producing areas of Ondo State vary from site to site. Th... more Microbes and mineral constituents of oil producing areas of Ondo State vary from site to site. The highest of 240 x 10 cfu/ml was recorded in Ayetoro compared with Sabomi having a low count record of 5 7x10 cfu/ml. Mineral element ranges from the lowest of 1.15ppm and 2.13ppm in Seluwa and Gbawiyo to highest 3 of 468.73ppm and 474.85ppm in Ayetoro and Seja for Ca; 0.24 and 3.52pp1m in areas like Sabomi and Araromi to 256.03ppm and 1185.34ppm in Ayetoro and Bijimi for Mg; 0.01 in Sabomi and Ilepete, Ayetoro, Ubale, Obeiji, Gbawiyo, Araromi, Abetobo, Pepe and Seluwa to 0.23 in Awoye, Ayetoro and Orioke for Mn; 0.07ppm in Ayetoro to 2.71 and 6.63ppm in Gbawiyo and Orioke for Fe; 0.01 in Igbokoda Jetty and Awoye, Ilepete, Ayetoro, Ubale, Obeiji, Gbawiyo, to 0.78ppm in Awoye and most stations studied for Lead (Pb). 0 values were recorded for PO4-P (ppm) in Ayetoro 1, Araromi, Bijimi and Sabomi to 0.49ppm in Awoye respectively. Other metal constituents in water samples are from the lowest of 64 ppm and 70.06 ppm in Gbawiyo and Awoye to highest of 440 and 400 ppm in Araromi and Obeiji for Cl; 0.1ppm in Awoye, Ilepete, Ayetoro, Araromi and Pepe 3 to 1.0 and 0.9ppm in Ayetoro and Awoye for Fluoride; 0.12ppm in Awoye to 0.31 and 0.3ppm in Ilepete for Cr; 0.04 in Ayetoro, Gbawiyo, Araromi and Pepe to 0.11 and 0.1 ppm in Awoye respectively for Cd. Sabomi (control site) showed low levels of most metals except calcium (Ca) compared with these of oil producing sampling site studied thus suggesting the metal constituent infiltration from oil pollutants in this area.

Advances in Life Sciences, 2014

The samples used for this study were obtained from different rhizosphere garden soil sources. Thi... more The samples used for this study were obtained from different rhizosphere garden soil sources. This include sweet potato rhizosphere soil (SPRS), plantain rhizosphere soil (PLRS), pineapple rhizosphere soil (PARS), pepper rhizosphere soil (PEPRS) and the control soil sample source (CSSS). The bacterial population determined by a pour plate technique showed a range of 4 x10-5 cfu/mL in control soil sample source (CSSS), to 29 x10-5 cfu/mL in sweet potato rhizosphere soil (SPRS). The bacterial isolates include Aerobacter spp.,

This study was focused on identifying and characterizing the ecological microbial communities in ... more This study was focused on identifying and characterizing the ecological microbial communities in
soil samples from Akoko communities in Akoko South West Local Government area, and Akure,
the State capital, Ondo State, Nigeria. Parameters such as soil temperature, pH, and some
biochemical characteristics of the microbial communities were determined. The total viable
bacterial counts estimated for this purpose ranged from 58 x 107 cfu gm-1 in Borehole soil sources
to 98 x 107 gm-1 in Stream sediment source. Similarly, some physiologic studies show that the
temperature of the soil samples ranged from 28.0°C to 30.1°C, while the pH for borehole sample is
pH6.09, stream, pH5.82 and river, pH6.25. The bacterial population of fish pond sources range
from 20 x 107 cfu gm-1 to 55 x 107 cfu gm-1. Twenty three bacterial isolates were obtained from
Akoko communities and this includes Bacillus spp., Acinetobacter spp., Eubacterium spp.
Staphylococcus spp., Proteus spp. Acidobacteria spp., Escherichia coli, Klebsiella spp.,
Flavobacterium spp. and Pseudomonas spp. In addition to this were six (6) bacterial and two (2)
fungal isolates obtained from pond soil sources in Akure, Ondo State, Nigeria. This is constituted of
Salmonella spp. Bacillus spp., Clostridium spp., Streptococcus spp., Enterococcus feacalis and
Lactobacillus spp. While the fungal isolates include Aspergillus niger and Aspergillus flavus. The
study will add to our knowledge of the microbial communities from different ecological sites in
Nigeria and can be helpful in environmental management and site improvements after disturbances
due to human interference and industrial developments.