Abdullahi T Ajao | Ahmadu Bello University, Nigeria (original) (raw)
Papers by Abdullahi T Ajao
International Journal of Life Sciences and Biotechnology
Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2020
American Journal of Bioinformatics Research, 2014
Malaria is a life-threatening disease and a major public health problem in Nigeria. According to ... more Malaria is a life-threatening disease and a major public health problem in Nigeria. According to the latest WHO estimates, there about 219 million cases of malaria in 2010 with an uncertainty range of 154 million to 289 million and an estimated 660,000 deaths mostly among African children. To date, there is no vaccine and the parasites are becoming resistant to available drugs, this necessitates the need for the identification vaccine candidates. Pharmacoproteomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Subtractive genomic approach was used to identify non-human homologous outer membrane proteins in P. falciparum. Four hypothetical proteins were identified based on Vaxijen score and exo-membrane topology, used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity. Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401. The epitopes are LLNNNMLGS, FSVTTNITI and FNVQYAAQL show high potential to induce both B-cell and T-cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against malaria. Experimental validation is required.
Bioinformation, 2012
Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of en... more Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of environmental pollutants through an important key biochemical pathways. Specifically, this enzyme cleaves aromatic rings of several environmental pollutants such as toluene, xylene, naphthalene and biphenyl derivatives. Hence, the importance of Catechol 2, 3-dioxygenase and its role in the degradation of environmental pollutants made us to predict the three-dimensional structure of Catechol 2, 3-dioxygenase from Burkholderia cepacia. The 10ns molecular dynamics simulation was carried out to check the stability of the modeled Catechol 2, 3- dioxygenase. The results show that the model was energetically stable, and it attains their equilibrium within 2000 ps of production MD run. The docking of various petroleum hydrocarbons into the Catechol 2,3-dioxygenase reveals that the benzene, O-xylene, Toluene, Fluorene, Naphthalene, Carbazol, Pyrene, Dibenzothiophene, Anthracene, Phenanthrene, Biphe...
Global Journal of Science Frontier …, 2011
Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of meth... more Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of methanolic extract of A. indica against sanitary indicator bacteria isolated from it was evaluated. Antibiotic susceptibility pattern and invitro therapeutic efficacy of Azadirachta indica stem extract was determined. This study indicated that the Kunnu samples were grossly contaminated with high plate count of 7.2 x 106 cfu/ml. Total Salmonella/Shigella plate count, Listeria monocytogenes counts, Total Staphylococcal count, total coliform count and E.coli were ranged between (12x101-7.6 x 103) cfu/ml, (4.8x102-25x104)cfu/ml, (9.4x101-1.0x104)cfu/ml (0.30x102-1.36x105)cfu/ml and (0.21x101-27x103)cfu/ml respectively. Erythromycin, Gentamycin, Cephalosporin, Ciprofloxacin and Chloramphenicol showed high level of antimicrobial activity against the tested isolates, while they displayed about 40% resistance to Penicillin, Streptomycin, Bacitracin, and Ampicillin. Methanolic extract of A. Indica stem showed inhibitory activity against all the bacterial isolates in which diameter of zone of inhibition, MIC and MBC ranged between 15-28mm, 3.125-50mg/ml and 3.125-100mg/ml respectively. E.coli showed highest zone of inhibition of 28mm with 3.125mg/ml and 6.25mg/ml of MIC and MBC respectively while pseudomonas sp showed lowest zone of inhibition of 15mm and 50-100mg/ml for MIC and MBC respectively. Control of both pathogens and spoilage bacteria in kunnu becomes important in order to produce food that when properly handled and stored, will have a long shelf life and reduce the incidence of food borne diseases.
Computational Biology and Bioinformatics, 2014
The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection thr... more The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection throughout the world and it is increasing public health concern. Infection caused by multidrug resistant A. baumannii is currently among the most difficult to treat due to propensity to acquire mobile genetic element. To date there is no vaccine or specific drug available for its treatment, this necessitate the need for the identification of therapeutic target enzyme and vaccine. Pharmacogenomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Comparative genomic approach was used to identify conserved protein signatures among five genomes. Three outer membrane proteins conserved among the genomes with high vaxijen scores were used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity.Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401.The epitopes are YEKLAAGPS, FYTSQPEDS and YVTGNPLGL with high potential to induce humoral and cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against A. baumannii infection, though experimental validation.
Bioinformation, 2012
Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of en... more Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of environmental pollutants through an important key biochemical pathways. Specifically, this enzyme cleaves aromatic rings of several environmental pollutants such as toluene, xylene, naphthalene and biphenyl derivatives. Hence, the importance of Catechol 2, 3-dioxygenase and its role in the degradation of environmental pollutants made us to predict the three-dimensional structure of Catechol 2, 3-dioxygenase from Burkholderia cepacia. The 10ns molecular dynamics simulation was carried out to check the stability of the modeled Catechol 2, 3dioxygenase. The results show that the model was energetically stable, and it attains their equilibrium within 2000 ps of production MD run. The docking of various petroleum hydrocarbons into the Catechol 2,3-dioxygenase reveals that the benzene, O-xylene, Toluene, Fluorene, Naphthalene, Carbazol, Pyrene, Dibenzothiophene, Anthracene, Phenanthrene, Biphenyl makes strong hydrogen bond and Van der waals interaction with the active site residues of H150,
The research aimed to investigate the bioremediation of spent engine oil on selected contaminated... more The research aimed to investigate the bioremediation of spent engine oil on selected contaminated soils within Ilorin metropolis. To achieve this, soil samples were collected from three (3) mechanic workshops along Taiwo axis within the metropolis. The soil samples were then subjected to bioremediation using the land-farming approach. The physicochemical parameters of the soil samples before and after bioremediation were analyzed using standard methods. Bacteria were isolated using standard procedures and identified using biochemical tests and molecular methods. Results for the physicochemical parameters of the soil samples before bioremediation include particle size (all sandy in nature); pH (6.00 ± 0.14 - 6.20 ± 0.14); Organic carbon (14.65 ± 3.20 - 17.54 ± 1.87), Organic matter (33.50 ± 0.85 - 43.45 ± 9.12) and heavy metals (ND - 11.74 ± 0.07). Values after bioremediation for pH, organic carbon, organic matter and heavy metals were 8.25 ± 0.07 - 8.90 ± 0.14, 13.07 ± 0.05 - 13.25 ...
Journal of Microbiology Research, 2014
Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worl... more Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worldwide causing ecological imbalance. Mineralization is essential for the conversion of petroleum hydrocarbon and hazardous pollutants into less toxic compounds, mostly water and carbon dioxide. In this study, refinery sludge samples from Kaduna Refining and Petrochemical Company were collected. These were analyzed by selective enrichment technique, resulted in the isolation of two bacterial species which are Burkholderia cepacia and Corynebacterium sp. Crude oil degradation was performed by the pure and mixed culture of the isolates incubated in the shake flask culture. Optical density, pH, Protein, Dehydrogenase and DHA activities were used as bioindicators for the degradation processes while CO2 production and GC-MS were used to analyze Mineralization potential. Dehydrogenase activities, Growth pattern, Protein estimation and FDA are strongly correlated with the rate of biodegradation t...
Fungi contamination of agricultural products had been one of the major concerns to human due to t... more Fungi contamination of agricultural products had been one of the major concerns to human due to their possibility of causing spoilage and the potential of producing potent toxins that could constitute health problems. Therefore, this study evaluates the effect of heat treatment on aflatoxin contents of tomatoes samples in some selected market in Ilorin, Kwara State, Nigeria. The spoilt and fresh tomatoes samples were randomly purchased from nine vendors for analysis. Fungi were isolated on Sabroud dextrose agar and identified using colonial and morphological characteristics. The levels of aflatoxin in the samples were determined using ELISA techniques and effects of heat treatments on aflatoxin levels of the samples were determined before and after 5, 15, 25 and 35 minutes of heat treatments at 100 oC. The results revealed the presence of fungi contaminants in all the analysed samples irrespective of their quality status. Furthermore, the aflatoxins contaminants were not detected in...
Natural and Applied Sciences Journal, Dec 29, 2018
The present study evaluates the bioremediation potential of indigenous bacterial species isolated... more The present study evaluates the bioremediation potential of indigenous bacterial species isolated from dye-contaminated soil samples from small dyeing outlet located in Ilorin. The water pollution index was estimated based on the physicochemical characteristics and heavy metal concentrations of the raw (Day 0) and treated textile wastewater such as pH, biochemical oxygen demand-5, chemical oxygen demand, total suspended solids and total dissolved solid with mean values of 8.85±0.45 mg/L, 1200±21.3 mg/L, 2440±31.3 mg/L, 1660±17.2 mg/L and 2650±28.1 mg/L respectively, similarly, Lead was the most abundant heavy metal detected in the sample while Cadmium concentration was the lowest with the mean values of 3.52±0.00 mg/L and 2.18±0.00 mg/L respectively. The bacterial strain with highest dye decolorization capacity was screened and identified as Bacillus licheniformis ZUL012.The isolate was consequently used for the bioremediation of the wastewater over a period of 10 days. The results showed an incredible reduction in the physiochemical characteristics and heavy metal concentrations of the textile wastewater in the following ranges (8.85-6.55), (1200-300) mg/L, (2440-518) mg/L, (1660-666) mg/L and (2650-920) mg/L with the highest removal efficiency of 75 %, 78 %, 60%, 65%, recorded for biochemical oxygen demand, chemical oxygen demand, total suspended solid, total dissolved solid, respectively while that of heavy metals such as lead, cadmium, chromium and nickel were 80 %, 60 %, 67 %, 72 % reduction, respectively. Laccase and Azoreductase activities tend to decrease as the pH gradually moved towards acidic condition during the bioremediation process. Toxicity of the treated effluent was assessed using Maize and Bean seed germination test. Conclusively, these research findings can serve as a framework for the outlet design of wastewater treatment plant for local textile outlets.
The present study evaluates the bioremediation potential of indigenous bacterial species isolated... more The present study evaluates the bioremediation potential of indigenous bacterial species isolated from dye-contaminated soil samples from small dyeing outlet located in Ilorin. The water pollution index was estimated based on the physicochemical characteristics and heavy metal concentrations of the raw (Day 0) and treated textile wastewater such as pH, biochemical oxygen demand-5, chemical oxygen demand, total suspended solids and total dissolved solid with mean values of 8.85±0.45 mg/L, 1200±21.3 mg/L, 2440±31.3 mg/L, 1660±17.2 mg/L and 2650±28.1 mg/L respectively, similarly, Lead was the most abundant heavy metal detected in the sample while Cadmium concentration was the lowest with the mean values of 3.52±0.00 mg/L and 2.18±0.00 mg/L respectively. The bacterial strain with highest dye decolorization capacity was screened and identified as Bacillus licheniformis ZUL012.The isolate was consequently used for the bioremediation of the ...
Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of meth... more Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of methanolic extract of A. indica against sanitary indicator bacteria isolated from it was evaluated. Antibiotic susceptibility pattern and invitro therapeutic efficacy of Azadirachta indica stem extract was determined. This study indicated that the Kunnu samples were grossly contaminated with high plate count of 7.2 x 106 cfu/ml. Total Salmonella/Shigella plate count, Listeria monocytogenes counts, Total Staphylococcal count, total coliform count and E.coli were ranged between (12x101 -7.6 x 103) cfu/ml, (4.8x102 -25x104)cfu/ml, (9.4x101 -1.0x104)cfu/ml (0.30x102 -1.36x105)cfu/ml and (0.21x101 -27x103)cfu/ml respectively. Erythromycin, Gentamycin, Cephalosporin, Ciprofloxacin and Chloramphenicol showed high level of antimicrobial activity against the tested isolates, while they displayed about 40% resistance to Penicillin, Streptomycin, Bacitracin, and Ampicillin. Methanolic extract of A. Indica stem showed inhibitory activity against all the bacterial isolates in which diameter of zone of inhibition, MIC and MBC ranged between 15-28mm, 3.125-50mg/ml and 3.125-100mg/ml respectively. E.coli showed highest zone of inhibition of 28mm with 3.125mg/ml and 6.25mg/ml of MIC and MBC respectively while pseudomonas sp showed lowest zone of inhibition of 15mm and 50-100mg/ml for MIC and MBC respectively. Control of both pathogens and spoilage bacteria in kunnu becomes important in order to produce food that when properly handled and stored, will have a long shelf life and reduce the incidence of food borne diseases.
The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection thr... more The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection throughout the world and it is increasing public health concern. Infection caused by multidrug resistant A. baumannii is currently among the most difficult to treat due to propensity to acquire mobile genetic element. To date there is no vaccine or specific drug available for its treatment, this necessitate the need for the identification of therapeutic target enzyme and vaccine. Pharmacogenomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Comparative genomic approach was used to identify conserved protein signatures among five genomes. Three outer membrane proteins conserved among the genomes with high vaxijen scores were used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity.Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401.The epitopes are YEKLAAGPS, FYTSQPEDS and YVTGNPLGL with high potential to induce humoral and cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against A. baumannii infection, though experimental validation.
Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worl... more Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worldwide causing ecological imbalance. Mineralization is essential for the conversion of petroleum hydrocarbon and hazardous pollutants into less toxic compounds, mostly water and carbon dioxide. In this study, refinery sludge samples from Kaduna Refining and Petrochemical Company were collected. These were analyzed by selective enrichment technique, resulted in the isolation of two bacterial species which are Burkholderia cepacia and Corynebacterium sp. Crude oil degradation was performed by the pure and mixed culture of the isolates incubated in the shake flask culture. Optical density, pH, Protein, Dehydrogenase and DHA activities were used as bioindicators for the degradation processes while CO 2 production and GC-MS were used to analyze Mineralization potential. Dehydrogenase activities, Growth pattern, Protein estimation and FDA are strongly correlated with the rate of biodegradation therefore; these parameters can be used as indicators for aerobic biodegradation of crude oil while This research confirmed that Biodegradation of petroleum hydrocarbon generally relies upon the cooperation of more than a single bacterial species, and such cooperation that exists between the isolates is quorum sensing phenomenon. These findings have environmental implication towards developing a microbial consortium that could be exploited for cleaning oil polluted environment.
Malaria is a life-threatening disease and a major public health problem in Nigeria. According to ... more Malaria is a life-threatening disease and a major public health problem in Nigeria. According to the latest WHO estimates, there about 219 million cases of malaria in 2010 with an uncertainty range of 154 million to 289 million and an estimated 660,000 deaths mostly among African children. To date, there is no vaccine and the parasites are becoming resistant to available drugs, this necessitates the need for the identification vaccine candidates. Pharmacoproteomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Subtractive genomic approach was used to identify non-human homologous outer membrane proteins in P. falciparum. Four hypothetical proteins were identified based on Vaxijen score and exo-membrane topology, used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity. Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401. The epitopes are LLNNNMLGS, FSVTTNITI and FNVQYAAQL show high potential to induce both B-cell and T-cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against malaria. Experimental validation is required.
Malaria is a life-threatening disease and a major public health problem in Nigeria. According to ... more Malaria is a life-threatening disease and a major public health problem in Nigeria. According to the latest WHO estimates, there about 219 million cases of malaria in 2010 with an uncertainty range of 154 million to 289 million and an estimated 660,000 deaths mostly among African children. To date, there is no vaccine and the parasites are becoming resistant to available drugs, this necessitates the need for the identification vaccine candidates. Pharmacoproteomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Subtractive genomic approach was used to identify non-human homologous outer membrane proteins in P. falciparum. Four hypothetical proteins were identified based on Vaxijen score and exo-membrane topology, used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity. Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401. The epitopes are LLNNNMLGS, FSVTTNITI and FNVQYAAQL show high potential to induce both B-cell and T-cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against malaria. Experimental validation is required.
Microbial activities are essential for the restoration of soil contaminated with hydrocarbons, th... more Microbial activities are essential for the restoration of soil contaminated with hydrocarbons, their roles includes biotransformation and mineralization of petroleum products into harmless compounds. In this study eighty six soil samples from fifteen auto-mechanic workshops within Ilorin metropolis were collected. These were analyzed by selective enrichment technique, resulted in the isolation of five bacterial species which are Acinetobacter sp, Flavobacterium sp, Pseudomonas sp., Serratia and Bacillus sp. Bioremediation of the soil microcosms was designed for 2months using mixed culture of the isolates. TVC, Protein, Dehydrogenase and lipase activities were used as bioindicators for the bioremediation processes. Highest TVC, Protein and Dehydrogenase activities were recorded in four week as 163.15µgTPFg -1 soil,6.3×10 7 and5.53mg/g were obtained respectively at pH of 7.17 and gradually declined while lipase activities and percentage of oil was highest in week six as 65.41% and4.72unit/g. These findings have environmental implication towards developing a bioremediation protocol that could be exploited for cleaning oil polluted soil @JASEM
Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of en... more Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of environmental pollutants through an important key biochemical pathways. Specifically, this enzyme cleaves aromatic rings of several environmental pollutants such as toluene, xylene, naphthalene and biphenyl derivatives. Hence, the importance of Catechol 2, 3-dioxygenase and its role in the degradation of environmental pollutants made us to predict the three-dimensional structure of Catechol 2, 3-dioxygenase from Burkholderia cepacia. The 10ns molecular dynamics simulation was carried out to check the stability of the modeled Catechol 2, 3dioxygenase. The results show that the model was energetically stable, and it attains their equilibrium within 2000 ps of production MD run. The docking of various petroleum hydrocarbons into the Catechol 2,3-dioxygenase reveals that the benzene, Ostrong hydrogen bond and Van der waals interaction with the active site residues of H150, L152, W198, H206, H220, H252, I254, T255, Y261, E271, L276 and F309. Free energy of binding and estimated inhibition constant of these compounds demonstrates that they are energetically stable in their binding cavity. Chrysene shows positive energy of binding in the active site atom of Fe. Except Pyrene all the substrates made close contact with Fe atom by the distance ranges from 1.67 to 2.43 Å. In addition to that, the above mentioned substrate except pyrene all other made π-π stacking interaction with H252 by the distance ranges from 3.40 to 3.90 Å. All these docking results reveal that, except Chrysene all other substrate has good free energy of binding to hold enough in the active site and makes strong VdW interaction with Catechol-2,3-dioxygenase. These results suggest that, the enzyme is capable of catalyzing the above-mentioned substrate.
International Journal of Life Sciences and Biotechnology
Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2020
American Journal of Bioinformatics Research, 2014
Malaria is a life-threatening disease and a major public health problem in Nigeria. According to ... more Malaria is a life-threatening disease and a major public health problem in Nigeria. According to the latest WHO estimates, there about 219 million cases of malaria in 2010 with an uncertainty range of 154 million to 289 million and an estimated 660,000 deaths mostly among African children. To date, there is no vaccine and the parasites are becoming resistant to available drugs, this necessitates the need for the identification vaccine candidates. Pharmacoproteomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Subtractive genomic approach was used to identify non-human homologous outer membrane proteins in P. falciparum. Four hypothetical proteins were identified based on Vaxijen score and exo-membrane topology, used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity. Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401. The epitopes are LLNNNMLGS, FSVTTNITI and FNVQYAAQL show high potential to induce both B-cell and T-cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against malaria. Experimental validation is required.
Bioinformation, 2012
Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of en... more Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of environmental pollutants through an important key biochemical pathways. Specifically, this enzyme cleaves aromatic rings of several environmental pollutants such as toluene, xylene, naphthalene and biphenyl derivatives. Hence, the importance of Catechol 2, 3-dioxygenase and its role in the degradation of environmental pollutants made us to predict the three-dimensional structure of Catechol 2, 3-dioxygenase from Burkholderia cepacia. The 10ns molecular dynamics simulation was carried out to check the stability of the modeled Catechol 2, 3- dioxygenase. The results show that the model was energetically stable, and it attains their equilibrium within 2000 ps of production MD run. The docking of various petroleum hydrocarbons into the Catechol 2,3-dioxygenase reveals that the benzene, O-xylene, Toluene, Fluorene, Naphthalene, Carbazol, Pyrene, Dibenzothiophene, Anthracene, Phenanthrene, Biphe...
Global Journal of Science Frontier …, 2011
Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of meth... more Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of methanolic extract of A. indica against sanitary indicator bacteria isolated from it was evaluated. Antibiotic susceptibility pattern and invitro therapeutic efficacy of Azadirachta indica stem extract was determined. This study indicated that the Kunnu samples were grossly contaminated with high plate count of 7.2 x 106 cfu/ml. Total Salmonella/Shigella plate count, Listeria monocytogenes counts, Total Staphylococcal count, total coliform count and E.coli were ranged between (12x101-7.6 x 103) cfu/ml, (4.8x102-25x104)cfu/ml, (9.4x101-1.0x104)cfu/ml (0.30x102-1.36x105)cfu/ml and (0.21x101-27x103)cfu/ml respectively. Erythromycin, Gentamycin, Cephalosporin, Ciprofloxacin and Chloramphenicol showed high level of antimicrobial activity against the tested isolates, while they displayed about 40% resistance to Penicillin, Streptomycin, Bacitracin, and Ampicillin. Methanolic extract of A. Indica stem showed inhibitory activity against all the bacterial isolates in which diameter of zone of inhibition, MIC and MBC ranged between 15-28mm, 3.125-50mg/ml and 3.125-100mg/ml respectively. E.coli showed highest zone of inhibition of 28mm with 3.125mg/ml and 6.25mg/ml of MIC and MBC respectively while pseudomonas sp showed lowest zone of inhibition of 15mm and 50-100mg/ml for MIC and MBC respectively. Control of both pathogens and spoilage bacteria in kunnu becomes important in order to produce food that when properly handled and stored, will have a long shelf life and reduce the incidence of food borne diseases.
Computational Biology and Bioinformatics, 2014
The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection thr... more The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection throughout the world and it is increasing public health concern. Infection caused by multidrug resistant A. baumannii is currently among the most difficult to treat due to propensity to acquire mobile genetic element. To date there is no vaccine or specific drug available for its treatment, this necessitate the need for the identification of therapeutic target enzyme and vaccine. Pharmacogenomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Comparative genomic approach was used to identify conserved protein signatures among five genomes. Three outer membrane proteins conserved among the genomes with high vaxijen scores were used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity.Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401.The epitopes are YEKLAAGPS, FYTSQPEDS and YVTGNPLGL with high potential to induce humoral and cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against A. baumannii infection, though experimental validation.
Bioinformation, 2012
Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of en... more Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of environmental pollutants through an important key biochemical pathways. Specifically, this enzyme cleaves aromatic rings of several environmental pollutants such as toluene, xylene, naphthalene and biphenyl derivatives. Hence, the importance of Catechol 2, 3-dioxygenase and its role in the degradation of environmental pollutants made us to predict the three-dimensional structure of Catechol 2, 3-dioxygenase from Burkholderia cepacia. The 10ns molecular dynamics simulation was carried out to check the stability of the modeled Catechol 2, 3dioxygenase. The results show that the model was energetically stable, and it attains their equilibrium within 2000 ps of production MD run. The docking of various petroleum hydrocarbons into the Catechol 2,3-dioxygenase reveals that the benzene, O-xylene, Toluene, Fluorene, Naphthalene, Carbazol, Pyrene, Dibenzothiophene, Anthracene, Phenanthrene, Biphenyl makes strong hydrogen bond and Van der waals interaction with the active site residues of H150,
The research aimed to investigate the bioremediation of spent engine oil on selected contaminated... more The research aimed to investigate the bioremediation of spent engine oil on selected contaminated soils within Ilorin metropolis. To achieve this, soil samples were collected from three (3) mechanic workshops along Taiwo axis within the metropolis. The soil samples were then subjected to bioremediation using the land-farming approach. The physicochemical parameters of the soil samples before and after bioremediation were analyzed using standard methods. Bacteria were isolated using standard procedures and identified using biochemical tests and molecular methods. Results for the physicochemical parameters of the soil samples before bioremediation include particle size (all sandy in nature); pH (6.00 ± 0.14 - 6.20 ± 0.14); Organic carbon (14.65 ± 3.20 - 17.54 ± 1.87), Organic matter (33.50 ± 0.85 - 43.45 ± 9.12) and heavy metals (ND - 11.74 ± 0.07). Values after bioremediation for pH, organic carbon, organic matter and heavy metals were 8.25 ± 0.07 - 8.90 ± 0.14, 13.07 ± 0.05 - 13.25 ...
Journal of Microbiology Research, 2014
Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worl... more Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worldwide causing ecological imbalance. Mineralization is essential for the conversion of petroleum hydrocarbon and hazardous pollutants into less toxic compounds, mostly water and carbon dioxide. In this study, refinery sludge samples from Kaduna Refining and Petrochemical Company were collected. These were analyzed by selective enrichment technique, resulted in the isolation of two bacterial species which are Burkholderia cepacia and Corynebacterium sp. Crude oil degradation was performed by the pure and mixed culture of the isolates incubated in the shake flask culture. Optical density, pH, Protein, Dehydrogenase and DHA activities were used as bioindicators for the degradation processes while CO2 production and GC-MS were used to analyze Mineralization potential. Dehydrogenase activities, Growth pattern, Protein estimation and FDA are strongly correlated with the rate of biodegradation t...
Fungi contamination of agricultural products had been one of the major concerns to human due to t... more Fungi contamination of agricultural products had been one of the major concerns to human due to their possibility of causing spoilage and the potential of producing potent toxins that could constitute health problems. Therefore, this study evaluates the effect of heat treatment on aflatoxin contents of tomatoes samples in some selected market in Ilorin, Kwara State, Nigeria. The spoilt and fresh tomatoes samples were randomly purchased from nine vendors for analysis. Fungi were isolated on Sabroud dextrose agar and identified using colonial and morphological characteristics. The levels of aflatoxin in the samples were determined using ELISA techniques and effects of heat treatments on aflatoxin levels of the samples were determined before and after 5, 15, 25 and 35 minutes of heat treatments at 100 oC. The results revealed the presence of fungi contaminants in all the analysed samples irrespective of their quality status. Furthermore, the aflatoxins contaminants were not detected in...
Natural and Applied Sciences Journal, Dec 29, 2018
The present study evaluates the bioremediation potential of indigenous bacterial species isolated... more The present study evaluates the bioremediation potential of indigenous bacterial species isolated from dye-contaminated soil samples from small dyeing outlet located in Ilorin. The water pollution index was estimated based on the physicochemical characteristics and heavy metal concentrations of the raw (Day 0) and treated textile wastewater such as pH, biochemical oxygen demand-5, chemical oxygen demand, total suspended solids and total dissolved solid with mean values of 8.85±0.45 mg/L, 1200±21.3 mg/L, 2440±31.3 mg/L, 1660±17.2 mg/L and 2650±28.1 mg/L respectively, similarly, Lead was the most abundant heavy metal detected in the sample while Cadmium concentration was the lowest with the mean values of 3.52±0.00 mg/L and 2.18±0.00 mg/L respectively. The bacterial strain with highest dye decolorization capacity was screened and identified as Bacillus licheniformis ZUL012.The isolate was consequently used for the bioremediation of the wastewater over a period of 10 days. The results showed an incredible reduction in the physiochemical characteristics and heavy metal concentrations of the textile wastewater in the following ranges (8.85-6.55), (1200-300) mg/L, (2440-518) mg/L, (1660-666) mg/L and (2650-920) mg/L with the highest removal efficiency of 75 %, 78 %, 60%, 65%, recorded for biochemical oxygen demand, chemical oxygen demand, total suspended solid, total dissolved solid, respectively while that of heavy metals such as lead, cadmium, chromium and nickel were 80 %, 60 %, 67 %, 72 % reduction, respectively. Laccase and Azoreductase activities tend to decrease as the pH gradually moved towards acidic condition during the bioremediation process. Toxicity of the treated effluent was assessed using Maize and Bean seed germination test. Conclusively, these research findings can serve as a framework for the outlet design of wastewater treatment plant for local textile outlets.
The present study evaluates the bioremediation potential of indigenous bacterial species isolated... more The present study evaluates the bioremediation potential of indigenous bacterial species isolated from dye-contaminated soil samples from small dyeing outlet located in Ilorin. The water pollution index was estimated based on the physicochemical characteristics and heavy metal concentrations of the raw (Day 0) and treated textile wastewater such as pH, biochemical oxygen demand-5, chemical oxygen demand, total suspended solids and total dissolved solid with mean values of 8.85±0.45 mg/L, 1200±21.3 mg/L, 2440±31.3 mg/L, 1660±17.2 mg/L and 2650±28.1 mg/L respectively, similarly, Lead was the most abundant heavy metal detected in the sample while Cadmium concentration was the lowest with the mean values of 3.52±0.00 mg/L and 2.18±0.00 mg/L respectively. The bacterial strain with highest dye decolorization capacity was screened and identified as Bacillus licheniformis ZUL012.The isolate was consequently used for the bioremediation of the ...
Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of meth... more Microbiological risk assessment of kunnu sold in Ilorin metropolis and therapeutic effect of methanolic extract of A. indica against sanitary indicator bacteria isolated from it was evaluated. Antibiotic susceptibility pattern and invitro therapeutic efficacy of Azadirachta indica stem extract was determined. This study indicated that the Kunnu samples were grossly contaminated with high plate count of 7.2 x 106 cfu/ml. Total Salmonella/Shigella plate count, Listeria monocytogenes counts, Total Staphylococcal count, total coliform count and E.coli were ranged between (12x101 -7.6 x 103) cfu/ml, (4.8x102 -25x104)cfu/ml, (9.4x101 -1.0x104)cfu/ml (0.30x102 -1.36x105)cfu/ml and (0.21x101 -27x103)cfu/ml respectively. Erythromycin, Gentamycin, Cephalosporin, Ciprofloxacin and Chloramphenicol showed high level of antimicrobial activity against the tested isolates, while they displayed about 40% resistance to Penicillin, Streptomycin, Bacitracin, and Ampicillin. Methanolic extract of A. Indica stem showed inhibitory activity against all the bacterial isolates in which diameter of zone of inhibition, MIC and MBC ranged between 15-28mm, 3.125-50mg/ml and 3.125-100mg/ml respectively. E.coli showed highest zone of inhibition of 28mm with 3.125mg/ml and 6.25mg/ml of MIC and MBC respectively while pseudomonas sp showed lowest zone of inhibition of 15mm and 50-100mg/ml for MIC and MBC respectively. Control of both pathogens and spoilage bacteria in kunnu becomes important in order to produce food that when properly handled and stored, will have a long shelf life and reduce the incidence of food borne diseases.
The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection thr... more The bacterial species Acinetobacter baumannii is a major cause of hospital acquired infection throughout the world and it is increasing public health concern. Infection caused by multidrug resistant A. baumannii is currently among the most difficult to treat due to propensity to acquire mobile genetic element. To date there is no vaccine or specific drug available for its treatment, this necessitate the need for the identification of therapeutic target enzyme and vaccine. Pharmacogenomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Comparative genomic approach was used to identify conserved protein signatures among five genomes. Three outer membrane proteins conserved among the genomes with high vaxijen scores were used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity.Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401.The epitopes are YEKLAAGPS, FYTSQPEDS and YVTGNPLGL with high potential to induce humoral and cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against A. baumannii infection, though experimental validation.
Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worl... more Environmental pollution with petroleum hydrocarbons has been recognized as a serious concern worldwide causing ecological imbalance. Mineralization is essential for the conversion of petroleum hydrocarbon and hazardous pollutants into less toxic compounds, mostly water and carbon dioxide. In this study, refinery sludge samples from Kaduna Refining and Petrochemical Company were collected. These were analyzed by selective enrichment technique, resulted in the isolation of two bacterial species which are Burkholderia cepacia and Corynebacterium sp. Crude oil degradation was performed by the pure and mixed culture of the isolates incubated in the shake flask culture. Optical density, pH, Protein, Dehydrogenase and DHA activities were used as bioindicators for the degradation processes while CO 2 production and GC-MS were used to analyze Mineralization potential. Dehydrogenase activities, Growth pattern, Protein estimation and FDA are strongly correlated with the rate of biodegradation therefore; these parameters can be used as indicators for aerobic biodegradation of crude oil while This research confirmed that Biodegradation of petroleum hydrocarbon generally relies upon the cooperation of more than a single bacterial species, and such cooperation that exists between the isolates is quorum sensing phenomenon. These findings have environmental implication towards developing a microbial consortium that could be exploited for cleaning oil polluted environment.
Malaria is a life-threatening disease and a major public health problem in Nigeria. According to ... more Malaria is a life-threatening disease and a major public health problem in Nigeria. According to the latest WHO estimates, there about 219 million cases of malaria in 2010 with an uncertainty range of 154 million to 289 million and an estimated 660,000 deaths mostly among African children. To date, there is no vaccine and the parasites are becoming resistant to available drugs, this necessitates the need for the identification vaccine candidates. Pharmacoproteomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Subtractive genomic approach was used to identify non-human homologous outer membrane proteins in P. falciparum. Four hypothetical proteins were identified based on Vaxijen score and exo-membrane topology, used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity. Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401. The epitopes are LLNNNMLGS, FSVTTNITI and FNVQYAAQL show high potential to induce both B-cell and T-cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against malaria. Experimental validation is required.
Malaria is a life-threatening disease and a major public health problem in Nigeria. According to ... more Malaria is a life-threatening disease and a major public health problem in Nigeria. According to the latest WHO estimates, there about 219 million cases of malaria in 2010 with an uncertainty range of 154 million to 289 million and an estimated 660,000 deaths mostly among African children. To date, there is no vaccine and the parasites are becoming resistant to available drugs, this necessitates the need for the identification vaccine candidates. Pharmacoproteomic and computational biology represent an attractive alternative approach for the identification of common drug target and peptide-vaccine candidates in the pathogen. Vaccine designing is shifted from entire pathogen or whole antigen to peptide or epitope based-vaccines that are specific, safe and easy to produce. Subtractive genomic approach was used to identify non-human homologous outer membrane proteins in P. falciparum. Four hypothetical proteins were identified based on Vaxijen score and exo-membrane topology, used to produce both B-cell and T-cell mediated immunity. Propred and propred1 were used to predict promiscuos helper T-Lymphocytes (HTL), Cytotoxic T-Lymphocyte (CTL) epitopes and MHCPred for their binding affinity. Three T-cell epitopes derived from identified B-cells bind to maximum number of MHC class I and class II alleles and specifically bind to HLA alleles such as DRB1*0101 and DRB1*0401. The epitopes are LLNNNMLGS, FSVTTNITI and FNVQYAAQL show high potential to induce both B-cell and T-cell mediated immune responses. These predicted epitopes (small peptide) might be promising candidates for vaccine design against malaria. Experimental validation is required.
Microbial activities are essential for the restoration of soil contaminated with hydrocarbons, th... more Microbial activities are essential for the restoration of soil contaminated with hydrocarbons, their roles includes biotransformation and mineralization of petroleum products into harmless compounds. In this study eighty six soil samples from fifteen auto-mechanic workshops within Ilorin metropolis were collected. These were analyzed by selective enrichment technique, resulted in the isolation of five bacterial species which are Acinetobacter sp, Flavobacterium sp, Pseudomonas sp., Serratia and Bacillus sp. Bioremediation of the soil microcosms was designed for 2months using mixed culture of the isolates. TVC, Protein, Dehydrogenase and lipase activities were used as bioindicators for the bioremediation processes. Highest TVC, Protein and Dehydrogenase activities were recorded in four week as 163.15µgTPFg -1 soil,6.3×10 7 and5.53mg/g were obtained respectively at pH of 7.17 and gradually declined while lipase activities and percentage of oil was highest in week six as 65.41% and4.72unit/g. These findings have environmental implication towards developing a bioremediation protocol that could be exploited for cleaning oil polluted soil @JASEM
Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of en... more Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of environmental pollutants through an important key biochemical pathways. Specifically, this enzyme cleaves aromatic rings of several environmental pollutants such as toluene, xylene, naphthalene and biphenyl derivatives. Hence, the importance of Catechol 2, 3-dioxygenase and its role in the degradation of environmental pollutants made us to predict the three-dimensional structure of Catechol 2, 3-dioxygenase from Burkholderia cepacia. The 10ns molecular dynamics simulation was carried out to check the stability of the modeled Catechol 2, 3dioxygenase. The results show that the model was energetically stable, and it attains their equilibrium within 2000 ps of production MD run. The docking of various petroleum hydrocarbons into the Catechol 2,3-dioxygenase reveals that the benzene, Ostrong hydrogen bond and Van der waals interaction with the active site residues of H150, L152, W198, H206, H220, H252, I254, T255, Y261, E271, L276 and F309. Free energy of binding and estimated inhibition constant of these compounds demonstrates that they are energetically stable in their binding cavity. Chrysene shows positive energy of binding in the active site atom of Fe. Except Pyrene all the substrates made close contact with Fe atom by the distance ranges from 1.67 to 2.43 Å. In addition to that, the above mentioned substrate except pyrene all other made π-π stacking interaction with H252 by the distance ranges from 3.40 to 3.90 Å. All these docking results reveal that, except Chrysene all other substrate has good free energy of binding to hold enough in the active site and makes strong VdW interaction with Catechol-2,3-dioxygenase. These results suggest that, the enzyme is capable of catalyzing the above-mentioned substrate.