Kisspeptin Signaling Is Indispensable for Neurokinin B, but not Glutamate, Stimulation of Gonadotropin Secretion in Mice (original) (raw)

Journal Article

,

1Department of Cell Biology, Physiology and Immunology (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

2University of Córdoba; CIBERobn Fisiopatología de la Obesidad y Nutrición (D.G.-G., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

3Instituto Maimonides de Investigaciones Biomédicas de Córdoba (IMIBIC) (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

Search for other works by this author on:

,

Dorette van Ingen Schenau

4Merck Sharp & Dohme (D.v.I.S., M.A.M.K.-F., P.I.v.N., M.B.), 5340 BH Oss, The Netherlands

Search for other works by this author on:

,

1Department of Cell Biology, Physiology and Immunology (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

3Instituto Maimonides de Investigaciones Biomédicas de Córdoba (IMIBIC) (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

Search for other works by this author on:

,

Magda A. M. Krajnc-Franken

4Merck Sharp & Dohme (D.v.I.S., M.A.M.K.-F., P.I.v.N., M.B.), 5340 BH Oss, The Netherlands

Search for other works by this author on:

,

1Department of Cell Biology, Physiology and Immunology (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

3Instituto Maimonides de Investigaciones Biomédicas de Córdoba (IMIBIC) (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

Search for other works by this author on:

,

1Department of Cell Biology, Physiology and Immunology (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

3Instituto Maimonides de Investigaciones Biomédicas de Córdoba (IMIBIC) (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

Search for other works by this author on:

,

1Department of Cell Biology, Physiology and Immunology (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

3Instituto Maimonides de Investigaciones Biomédicas de Córdoba (IMIBIC) (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

Search for other works by this author on:

,

1Department of Cell Biology, Physiology and Immunology (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

2University of Córdoba; CIBERobn Fisiopatología de la Obesidad y Nutrición (D.G.-G., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

3Instituto Maimonides de Investigaciones Biomédicas de Córdoba (IMIBIC) (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

Search for other works by this author on:

,

4Merck Sharp & Dohme (D.v.I.S., M.A.M.K.-F., P.I.v.N., M.B.), 5340 BH Oss, The Netherlands

Search for other works by this author on:

,

1Department of Cell Biology, Physiology and Immunology (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

2University of Córdoba; CIBERobn Fisiopatología de la Obesidad y Nutrición (D.G.-G., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

3Instituto Maimonides de Investigaciones Biomédicas de Córdoba (IMIBIC) (D.G.-G., S.L., M.M.-L., A.R.-R., V.M.N., F.G., L.P., M.T.-S.), 14004 Córdoba, Spain

Search for other works by this author on:

... Show more

Accepted:

17 October 2011

Published:

01 January 2012

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

David García-Galiano, Dorette van Ingen Schenau, Silvia Leon, Magda A. M. Krajnc-Franken, Maria Manfredi-Lozano, Antonio Romero-Ruiz, Victor M. Navarro, Francisco Gaytan, Paula I. van Noort, Leonor Pinilla, Marion Blomenröhr, Manuel Tena-Sempere, Kisspeptin Signaling Is Indispensable for Neurokinin B, but not Glutamate, Stimulation of Gonadotropin Secretion in Mice, Endocrinology, Volume 153, Issue 1, 1 January 2012, Pages 316–328, https://doi.org/10.1210/en.2011-1260
Close

Navbar Search Filter Mobile Enter search term Search

Kisspeptins (Kp), products of the Kiss1 gene that act via Gpr54 to potently stimulate GnRH secretion, operate as mediators of other regulatory signals of the gonadotropic axis. Mouse models of Gpr54 and/or Kiss1 inactivation have been used to address the contribution of Kp in the central control of gonadotropin secretion; yet, phenotypic and hormonal differences have been detected among the transgenic lines available. We report here a series of neuroendocrine analyses in male mice of a novel Gpr54 knockout (KO) model, generated by heterozygous crossing of a loxP-_Gpr54/_Protamine-Cre double mutant line. Gpr54-null males showed severe hypogonadotropic hypogonadism but retained robust responsiveness to GnRH. Gonadotropic responses to the agonist of ionotropic glutamate receptors, _N_-methyl-d-aspartate, were attenuated, but persisted, in Gpr54-null mice. In contrast, LH secretion after activation of metabotropic glutamate receptors was totally preserved in the absence of Gpr54 signaling. Detectable, albeit reduced, LH responses were also observed in Gpr54 KO mice after intracerebroventricular administration of galanin-like peptide or RF9, putative antagonist of neuropeptide FF receptors for the mammalian ortholog of gonadotropin-inhibiting hormone. In contrast, the stimulatory effect of senktide, agonist of neurokinin B (NKB; cotransmitter of Kiss1 neurons), was totally abrogated in Gpr54 KO males. Lack of Kp signaling also eliminated feedback LH responses to testosterone withdrawal. However, residual but sustained increases of FSH were detected in gonadectomized Gpr54 KO males, in which testosterone replacement failed to fully suppress circulating FSH levels. In sum, our study provides novel evidence for the relative importance of Kp-dependent vs. -independent actions of several key regulators of GnRH secretion, such as glutamate, galanin-like peptide, and testosterone. In addition, our data document for the first time the indispensable role of Kp signaling in mediating the stimulatory effects of NKB on LH secretion, thus supporting the hypothesis that NKB actions on GnRH neurons are indirectly mediated via its ability to regulate Kiss1 neuronal output.

Copyright © 2012 by The Endocrine Society

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Kisspeptin Signaling Is Indispensable for Neurokinin B, but not Glutamate, Stimulation of Gonadotropin Secretion in Mice - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 1,835

1,290 Pageviews

545 PDF Downloads

Since 2/1/2017

Citations

133 Web of Science

×

Email alerts

More on this topic

Related articles in PubMed

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic