Lysogenic Transfer of Group A Streptococcus Superantigen Gene among Streptococci (original) (raw)

Journal Article

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1

Max F. Perutz Laboratories, University of Vienna, Department of Microbiology and Immunobiology

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Vienna, Austria

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1

Max F. Perutz Laboratories, University of Vienna, Department of Microbiology and Immunobiology

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Vienna, Austria

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Birgitta Henriques-Normark

2

Swedish Institute for Infectious Disease Control, Department of Bacteriology

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Solna, Sweden

3

Karolinska Institute

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Solna, Sweden

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Max F. Perutz Laboratories, University of Vienna, Department of Microbiology and Immunobiology

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Vienna, Austria

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1

Max F. Perutz Laboratories, University of Vienna, Department of Microbiology and Immunobiology

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Vienna, Austria

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Max F. Perutz Laboratories, University of Vienna, Department of Microbiology and Immunobiology

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Vienna, Austria

Reprints or correspondence: Dr. Emmanuelle Charpentier, Max F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9/4, 1030 Vienna, Austria (emmanuelle.charpentier@univie.ac.at).

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Published:

15 January 2008

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Ivo Vojtek, Zaid A. Pirzada, Birgitta Henriques-Normark, Markus Mastny, Rajendra P. Janapatla, Emmanuelle Charpentier, Lysogenic Transfer of Group A Streptococcus Superantigen Gene among Streptococci, The Journal of Infectious Diseases, Volume 197, Issue 2, 15 January 2008, Pages 225–234, https://doi.org/10.1086/524687
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Abstract

A group A Streptococcus (GAS) isolate, serotype M12, recovered from a patient with streptococcal toxic shock syndrome was analyzed for superantigen-carrying prophages, revealing φ149, which encodes superantigen SSA. Sequence analysis of the att-L proximal region of φ149 showed that the phage had a mosaic nature. Remarkably, we successfully obtained lysogenic conversion of GAS clinical isolates of various M serotypes (M1, M3, M5, M12, M19, M28, and M94), as well as of group C Streptococcus equisimilis (GCSE) clinical isolates, via transfer of a recombinant phage φ149::Kmr. Phage φ149::Kmr from selected lysogenized GAS and GCSE strains could be transferred back to M12 GAS strains. Our data indicate that horizontal transfer of lysogenic phages among GAS can occur across the M-type barrier; these data also provide further support for the hypothesis that toxigenic conversion can occur via lysogeny between species. Streptococci might employ this mechanism specifically to allow more efficient adaptation to changing host challenges, potentially leading to fitter and more virulent clones.

© 2008 by the Infectious Diseases Society of America

Topic:

• streptococcus pyogenes
• bacteriophages
• clone cells
• genes
• lysogeny
• sequence analysis
• streptococcus
• superantigens
• mosaicism
• streptococcus group c
• streptococcal toxic shock syndrome
• toxigenic
• grand mal status epilepticus
• transfer technique
• host (organism)
• serotype
• calr gene

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